Journal of Plant Biotechnology

  • 제33권4호
  • /
  • Pages.249-255
  • /
  • 2006
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Effect of Ethylene Inhibitors on In Vitro Shoot Multiplication and their Impact on Ethylene Production in Cucumber (Cucumis sativus L.)

  • Vasudevan A. (Department of Biology and Medicinal Science, Pai Chai University) ;
  • Selvaraj N. (Department of Botany, Periyar E.V.R. College(Autonomous)) ;
  • Ganapathi A. (Department of Biotechnology, Bharathidasan University) ;
  • Anbazhagan V. Ramesh (Division of Forest Resources, College of Forest Sciences, Kangwon National University) ;
  • Choi, C.W. (Department of Biology and Medicinal Science, Pai Chai University)
  • 발행 : 2006.12.30
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초록

Effects of ethylene inhibitors like silver nitrate $(AgNO_3)$, cobalt chloride $(CoCl_2)$ and Salicylic acid (SA) on multiple shoot induction and their impact on ethylene production using embryonal cotyledon cultures of Cucumis sativus L. were examined. The optimum concentration of $AgNO_3\;(40{\mu}M),\;CoCl_2\;(20{\mu}M)\;and\;SA\;(20{\mu}M)$, separately, induced maximum number of shoots on Murashige and Skoog's (MS) medium supplemented optimally with $4.44{\mu}M$ BA and $0.25{\mu}M$ NAA. Among the three ethylene inhibitors tested, $AgNO_3$ produced maximum number of shoots when compared to $CoCl_2$ and SA Ethylene production was monitored in all the treatments with $AgNO_3/CoCl_2/SA$ and it was observed that the treatment with $AgNO_3$ alone showed increase in ethylene production when compared to $CoCl_2$ and SA Even though ethylene concentration was the highest in $AgNO_3$ treated explants, maximum number of shoots was obtained.

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참고문헌

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