Immunological Study of the Gami-sopunghwalhyeol-tang ($Ji{\={a}}w{\`{e}}i-sh{\={u}}f{\={e}}nghu{\`{o}}xu{\`{e}}-tang$: GSHT) on Rheumatoid Arthritis in Human Fibroblast-like Synoviocytes

가미소풍활혈탕(加味疎風活血湯)이 류마토이드 관절염에 미치는 실험적 연구

  • Kim, Tae-Young (Dept of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dae-jeon University) ;
  • Song, Young-Il (Dept of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dae-jeon University) ;
  • Oh, Min-Suck (Dept of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dae-jeon University) ;
  • Yoon, Il-Ji (Dept of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dae-jeon University)
  • 김태영 (대전대학교 한의과대학 한방재활의학과교실) ;
  • 송영일 (대전대학교 한의과대학 한방재활의학과교실) ;
  • 오민석 (대전대학교 한의과대학 한방재활의학과교실) ;
  • 윤일지 (대전대학교 한의과대학 한방재활의학과교실)
  • Published : 2006.09.30

Abstract

Objectives: This study was carried out to find the immune responses of the Gami-sopunghwalhyeol-tang $(Ji{\={a}}w{\`{e}}i-sh{\={u}}f{\={e}}nghu{\'{o}}xu{\`{e}}-tang)$ (hereinafter referred to GSHT) to the human fibroblast-like synoviocytes (hFLSs) isolated from patients with rheumatoid arthritis. Methods: Experiments were performed to measure the cytotoxity against hFCs and the production of pro-inflammatory cytokines in hFLSs and the production of NO, ROS. Results: 1. The gene expression of TNF-a, IL-6, IL-8 in hFLSs was effectively reduced at $100{\mu}g/ml$, whereas IL-1 $\beta$ was effectively reduced at 100 and $10{\mu}g/ml$ of GSHT. 2. The gene expression of ICAM-1, MMP-3 in hFLSs was effectively inhibited at 100 and $10{\mu}g/ml$ of GSHT, whereas TIMP-1 was effectively increased at 100 and $10{\mu}g/ml$ of GSHT. 3. The gene expression of NOS-II in hFLSs was effectively inhibited at $100{\mu}g/ml$ of GSHT. 4. The production of NO and ROS in hFLSs was inhibited at 100 and $10{\mu}g/ml$ of GSHT. 5. The proliferation of hFLSs was significantly inhibited at $100{\mu}g/ml$ of GSHT. Conclusions: Comparison of the results for this study showed that Gami-sopunghwalhyeol-tang ($Ji{\={a}}w{\`{e}}i-sh{\={u}}f{\={e}}nghu{\'{o}}xu{\`{e}}-tang$: GSHT) had immunomodulatory effects of suppressing or enhancing.

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