Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Pulsed-Field Gel Electrophoresis-Based Molecular Typing Reveals a Shift in the Major Type of Vibrio cholerae O1 Isolated in Korea

  • Kim, Seong-Han (Division of Enteric Bacterial Infections, Centers for Infections Disease, Korea, National Institute of Health) ;
  • Kim, Jun-Young (Division of Enteric Bacterial Infections, Centers for Infections Disease, Korea, National Institute of Health) ;
  • Kang, Yeon-Ho (Division of Enteric Bacterial Infections, Centers for Infections Disease, Korea, National Institute of Health) ;
  • Park, Yong-Keun (Graduate School of Biotechnology, Korea University) ;
  • Lee, Bok-Kwon (Division of Enteric Bacterial Infections, Centers for Infections Disease, Korea, National Institute of Health)
  • Published : 2006.11.30
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Abstract

Vibrio cholerae O1 El Tor isolates (n=242), collected in Korea between 1991 and 2002, were classified by NotI-digested pulsed-field gel electrophoresis (PFGE). The major types were Al before 1998 and B after 1999, among both domestic and imported cases. The prevalent PFGE types among domestic cases were consistent with the prevalent types among imported cases in the same year. These results suggest a close relationship between the domestic and imported cases of cholera in Korea.

Keywords

References

  1. Amita, S., R. Chowdhury, M. Thungapathra, T. Ramamurthy, N. Blalakrish, and A. Ghosh. 2003. Class I integrons and SXT elements in El Tor strains, isolated before and after 1992 Vibrio cholerae O139 outbreak, Calcutta, India. Emerg. Infect. Dis. 9: 500-502 https://doi.org/10.3201/eid0904.020317
  2. Cameron, K. N., F. M. Khambaty, I. K. Waxhsmuth, R. V. Tauxe, and T. J. Barrett. 1994. Molecular characterization of Vibrio cholerae O1 strains by pulsed-field gel electrophoresis. J. Clin. Microbiol. 30: 1685-1690
  3. Dalsgaard, A., A. Forslund, D. Sandvang, L. Arntzen, and K. Keddy. 2001. Vibrio cholerae O1 outbreak isolates in Mozambique and South Africa in 1998 are multiple-drug resistant, contain the SXT element and the aadA2 gene located on class 1 integrons. J. Antimicrob. Chemother. 42: 827-838
  4. Gautom, R. 1997. Rapid PFGE protocol for typing of E. coli O157:H7 and other gram-negative organism in 1 day. J. Clin. Microbiol. 35: 2977-2980
  5. Kim, H., Y. Shin, Y. Kang, C. Yoo, M. Park, J. Yu, and T. Jung. 1996. Epidemiological aspect of Vibrio cholerae strains recently imported and cholera epidemic outbreak among indigenous Korean residents in 1995. Infections 28: 473-479. (In Korean)
  6. Matsumoto, M., M. Suzuki, R. Hiramatsu, M. Yamazak, H. Matsui, K. Sakae, Y. Suzuki, and Y. Miyazaki. 2002. Epidemiological investigation of a fatal case of cholera in Japan by phenotypic technique and pulsed-field gel electrophoresis. J. Med. Microbiol. 51: 264-268 https://doi.org/10.1099/0022-1317-51-3-264
  7. National Committee for Clinical Laboratory Standards. 2000. Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard, 7th ed. National Committee for Clinical Laboratory Standards, Wayne, PA
  8. National Institute of Health in Korea. 2001. Epidemic characteristics of epidemic cholera in Korea, in 2001. Communicable Dis. Monthly Rep. 12: 119-120
  9. Southern, E. M. 1975. Detection of specific sequences among DNA fragments separated by agarose gel electrophoresis. J. Mol. Biol. 98: 503-517 https://doi.org/10.1016/S0022-2836(75)80083-0
  10. Tenover, F. C., R. D. Arbeit, R. V. Goering, P. A. Mickelesen, B. E. Murray, D. H. Persing, and B. Swaminathan. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: Criteria for bacterial strain typing. J. Clin. Microbiol. 33: 2233-2239
  11. Waldor, M. K., H. Tschape, and J. J. Mekalanos. 1996. A new type of conjugative transposon encodes resistance to sulfamethoxazole, trimethoprime, and streptomycin in Vibrio cholerae O139. J. Bacteriol. 178: 4157-4165 https://doi.org/10.1128/jb.178.14.4157-4165.1996