Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
권호 표지

Effect of Intra-Uterine Infusion of Lipopolysaccharides on Reproductive Performance in Dairy Cows with Retained Placenta

후산정체 젖소에 있어서 Lipopolysaccharide의 처리가 번식 성적에 미치는 영향

  • Baek, Kwang-Soo (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Park, Seong-Jai (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Park, Soo-Bong (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Lee, Wang-Sik (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Kim, Hyeon-Shup (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Jeong, Gyeong-Yong (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Ki, Kwang-Seok (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Jeon, Byeong-Soon (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Ah, Byeong-Seog (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Suh, Guk-Hyun (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Kim, Tae-Il (Dairy Cattle Research Division, National Livestock Research Institute, RDA)
  • 백광수 (농촌진흥청 축산연구소 낙농과) ;
  • 박성재 (농촌진흥청 축산연구소 낙농과) ;
  • 박수봉 (농촌진흥청 축산연구소 낙농과) ;
  • 이왕식 (농촌진흥청 축산연구소 낙농과) ;
  • 김현섭 (농촌진흥청 축산연구소 낙농과) ;
  • 정경용 (농촌진흥청 축산연구소 낙농과) ;
  • 기광석 (농촌진흥청 축산연구소 낙농과) ;
  • 전병순 (농촌진흥청 축산연구소 낙농과) ;
  • 안병석 (농촌진흥청 축산연구소 낙농과) ;
  • 서국현 (농촌진흥청 축산연구소 낙농과) ;
  • 김태일 (농촌진흥청 축산연구소 낙농과)
  • Published : 2006.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to investigate the effect intra-uterine infusion of lipopolysaccharides(LPS) on the reproductive performance of dairy cows with retained placenta, The LPS isolated from Bacteroids helcogenes and Fusobacterium varium was injected at the rate of 100 ${\mu}g$ with 30 ml of phospahte buffer saline in each cow(n=33) at day 20 postpartum. Twelve cows with retained placenta were not heated with LPS and served as a control. Conception rate after first service was 0% and 33,3% for control and LPS groups, respectively. Service period was $149.6{\pm}34.3\;and\;53.0{\pm}12.5$ days for control and LPS groups, respectively(p<0.05). Services per conception was $3.6{\pm}0.8\;and\;2.1{\pm}0.3$ for control and LPS groups, respectively(p<0.05). In conclusion, intra-uterine infusion of LPS in cows with retained placenta can reduce the service period and services per conception. Reduced service period can improve the dairy farm profitability by decreasing cost un feeding and management of cows.

본 연구는 후산정체 젖소에 있어서 Lipopolysaccharide(LPS)의 처리가 번식 성적에 미치는 영향을 구명하기 위하여 분만 후 12시간이 경과하여도 후산이 배출되지 않은 홀스타인 개체 33두 및 대조구 12두에 대하여 분만후 20일째에 LPS 100 ${\mu}g$을 자궁 내에 주입한 다음 7일후에 발정이 발현되었을 때 인공수정을 실시하여 다음과 같은 결과를 얻었다. 후산정체 젖소에 있어서 첫 수정에 의한 수태율은 대조구의 경우 공시한 12두가 모두 첫 수정에 의해 수태가 되지 않았고 처리구는 33두중 11두가 수태되어 33.3%의 수태율을 나타내었다. 후산정체 젖소에 있어서 대조구 및 처리구의 분만후 수태까지의 일수는 각각 $149.6{\pm}34.3$일 및 $53.0{\pm}12.5$일로 처리구가 대조구에 비하여 유의적(p<0.01)으로 단축되는 경향을 나타내었다. 후산정체 젖소에 있어서 대조구 및 처리구의 수태당 종부 횟수는 각각 $3.6{\pm}0.8$회 및 $2.1{\pm}0.3$회로 처리구가 대조구에 비하여 유의적(p<0.05)으로 낮은 경향을 나타내었다.

Keywords

References

  1. Ahlers D, Grunert E (1993): Zur Problematik der Behandlung des infizierten Uterus beim Rind im Puerperium. Prakt. Tierarzt Sondert. Coll Vet. XXIV, pp 57-62
  2. Bajcsy AC, Szenci O, Gijsbert C, van der Weijden, Doornenbal A, Maassen F, Bartyik J, Marcel AM, Taverne (2006): The effect of a single oxytocin or carbetocin treatment on uterine contractility in early postpartumn dairy cows. Theriogenology 65:400-414 https://doi.org/10.1016/j.theriogenology.2005.05.040
  3. Choi KM, Kim BK (1998): Studies on the etiology of ratained fetal membrane in the Korean native cattles. Korean J Dairy Sci 10:72-76
  4. Dadarwal D, Tandon SN, Purohit GN, Pareek PK (2004): Ultrasonographic evaluation of uterine involution and postpartum follicular dynamics in French Jennies (Equus asinus). Theriogenology 62:257-264 https://doi.org/10.1016/j.theriogenology.2003.10.005
  5. Dobson DP, Noakes DE (1990): Use of a uterine pessary to prevent infection of the uterus of the cow after parturition. Vet Rec 127:128-131
  6. Dhaliwal GS, Murray RD, Woldehiwet Z (2001): Some aspects of immunology of the bovine uterus related to treatments for endometritis. Anim Reprod Sci 67:135-152 https://doi.org/10.1016/S0378-4320(01)00124-5
  7. Dohmen MJW, Huszenicza GY, Fodor M, Kulcsar M, Vamos M, Porkolab L, Szilagyi N, Lohuis JACM (1996): Bacteriology and fertility in healthy postpartum cows and cows with acute endometritis. Proc. XIX world Buiatrics Congress, pp 238- 241
  8. Dohmen MJW, Lohuis JACM, Huszenicza Gy, Nagy P, Gacs M (1995): The relationship between bacteriological and clinical findings in cows with subacute/ chronic endometritis. Theriogenology 43:1379-1388 https://doi.org/10.1016/0093-691X(95)00123-P
  9. Drillich M, Beetz O, pfutzner A, Sabin M, Sabin HJ, Kutzer P, Natterrnann H, Heuwieser W (2001): Evaluation of a systemic antibiotic treatment of toxic puerperal metritis in dairy cows. Am Dairy Sci Assoc 84:2010-2017
  10. Drillich M, Mahlstedt M, Reichert U, Tenhagen BA, Heuwieser W (2006a): Strategies to Improve the therapy of retained fetal membranes in dairy cows. J Dairy Sci 89:627-635 https://doi.org/10.3168/jds.S0022-0302(06)72126-9
  11. Drillich M, Reichert U, Mahlstedt M, Heuwieser W (2006b): Comparison of two strategies for systemic antibiotic treatment of dairy cows with retained fetal membranes: Preventive vs. Selective Treatment. Am Dairy Sci Assoc 89:1502-1508
  12. Francos G, Mayer B (1988): Analysis of fertility indices of cows with extended postpartwn anestrus and other reproductive disorders compared to normal cows. Theriogenology 29:399-412 https://doi.org/10.1016/0093-691X(88)90243-9
  13. Frank T, Anderson KL, Smith AR, Whitmore HL, Gustafsson B (1983): Phagocytosis in the uterus: A review, Theriogenology 20:103-110 https://doi.org/10.1016/0093-691X(83)90029-8
  14. Griffin JFT, Hartigan PJ, Nunn WR (1974): Nonspecific uterine infection and bovine fertility-I. Infection patterns and endometritis during the first seven weeks post-partum. Theriogenology 1:91-106 https://doi.org/10.1016/0093-691X(74)90052-1
  15. Gross TS, Williams WF, Moreland TW (1986): Prevention of the retained fetal membrane syndrome (retained placenta) during induced calving in dairy cattle. Theriogenology 26:365-370 https://doi.org/10.1016/0093-691X(86)90156-1
  16. Heuwieser W, Tenhagen BA, Tischer M, Luhr J, Blum H (2000): Effect of three programmes for the treatment of endometritis on the reproductive performance of a dairy herd. Vet Rec 146:338-341 https://doi.org/10.1136/vr.146.12.338
  17. Hill AW, Hibbit KG, Shears AL (1976): Increased antibacterial activity against E. coli in serum after the induction of endotoxin tolerance. Infect Immun 14:257-265
  18. Holt LC, Whittier WD, Gwazdauskas FC, Vinson WE (1989): Early postpartum reproductive profiles in Holstein cows with retained placenta and uterine discharges. J Dairy Sci 72:533-539 https://doi.org/10.3168/jds.S0022-0302(89)79137-2
  19. Hussain AM, Daniel RCW, O'Boyle D (1990): Postpartum uterine flora following normal and abnormal puerperium in cows. Theriogenology 34:291-302 https://doi.org/10.1016/0093-691X(90)90522-U
  20. Hussain AM, Daniel RCW (1991): Bovine endometritis: Current and future alternative therapy. J Vet Med A 38:641-651 https://doi.org/10.1111/j.1439-0442.1991.tb01060.x
  21. Husssain AM, Daniel RCW (1992): Effects of intrauterine infusion of Escherichia coli endotoxin in normal cows and in cows with endometritis induced by experimental infection with Streptococcus agalactiae. Theriogenology 37:791-810 https://doi.org/10.1016/0093-691X(92)90042-P
  22. Jainudeen MR, Hafez ESE (1993): Reproductive failure in females. In reproduction in farm animals. 6th ed. E.S.E. Hafez(ed). Philadelphia Lea & Febiger, pp 261-286
  23. Landaeta-Hernandez AJ, Giangreco M, Melendez P, Bartolome J, Bennet F, Rae DO, Hernandez J, Archbald LF (2004): Effect of biostimulation on uterine involution, early ovarian activity and first postpartum estrous cycle in beef cows. Theriogenology 61:1521-1532 https://doi.org/10.1016/j.theriogenology.2003.09.002
  24. LeBlanc SJ, Duffield TF, Leslie KE, Bateman KG, Keefe GP, Walton JS, Johnson WH (2002): The effect of treatment of clinical endometritis on reproductive performance in dairy cows. Am Dairy Sci Assoc 85:2237-2249
  25. Lindell JO, Kindahl H, Jansson L, Edqvist LE (1982): Post-partum release of prostaglandin $F_{2a}$ and uterine involution in the cow. Theriogenology 17:237-245 https://doi.org/10.1016/0093-691X(82)90085-1
  26. Melendez P, McHale J, Bartolome J, Archbald LF, Donovan GA (2004): Uterine involution and fertility of Holstein cows subsequent to early postpartum $PGF_{2a}$ treatment for acute puerperal metritis. Am Dairy Sci Assoc 87:3238-3246
  27. Nakao T, Moriyoshi M, Kawata K (1992): The effect of postpartum ovarian dysfunction and endometritis on subsequent reproductive performance in high and medium producing dairy cows. Theriogenology 37:341-349 https://doi.org/10.1016/0093-691X(92)90192-T
  28. Okano A, Tomizuka T (1987): Ultrasonic observation of postpartum uterine involution in the cow. Theriogenology 27:369-376 https://doi.org/10.1016/0093-691X(87)90225-1
  29. Okuda K, Gaona WA, Sato K (1988): Effects of gonadotropin releasing hormone and prostaglandin $F_{2a}$ on the reproductive performance in postpartum cows. Theriogenology 29:823-833 https://doi.org/10.1016/0093-691X(88)90219-1
  30. Risco CA, Drost M, Thatcher WW, Savio J, Thatcher MJ (1994): Effects of calving-related disorders on prostaglandin, calcium, ovarian activity and uterine involution in postpartum dairy cows. Theriogenology 42:183-203 https://doi.org/10.1016/0093-691X(94)90675-9
  31. Sheldon IM, Noakes DE, Dobson H (2000): The influence of ovarian activity and uterine involution determined by ultrasonography on subsequent reproductive performance. Theriogenology 54:409-419 https://doi.org/10.1016/S0093-691X(00)00358-7
  32. Singh J, Sidhu SS, Dhaliwal GS, Pangaonkar GR, Nanda AS, Grewal AS (2000): Effectiveness of lipopolysaccharide as an intrauterine immunornodula- tor in curing bacterial endometritis in repeat breeding cross-bred cows. Anim Reprod Sci 59:159- 166 https://doi.org/10.1016/S0378-4320(00)00144-5
  33. Squires AG (1980): Current therapy in Theriogenology 1 Ed. Morrow DA, Philadelphia. Saunders WB, p 186
  34. Takacs T, Gathy I, Machaty Z, Bajmocy E (1990): Bacterial contamination of the uterus after parturition and its effect on the reproductive performance of cows on large-scale dairy farms. Theriogenology 33:851-865 https://doi.org/10.1016/0093-691X(90)90821-A
  35. Usmani RH, Ahmad N, Shafiq P, Mirza MA (2001):Effect of subclinical uterine infection on cervical and uterine involution, estrous activity and fertility in postpartum buffaloes. Theriogenology 55:563-571 https://doi.org/10.1016/S0093-691X(01)00426-5
  36. 류병호, 박우열, 김희숙, 박종옥(1991): Proteus vulgaris RH-90에서 추출하여 감마선 조사시킨 Lipopolysaccharide(LPS)의 항암 및 면역 활성에 미치는 영향. 한국생물공학회지 6:45-54
  37. 정기화(1990): 젖소에 있어서 후산정체의 발생원인과 예방에 관한 연구. 경상대학교 박사학위청구논문