Efficacy of Cyanobacterial Biofertilizer (CBB) on Leaf Yield and Quality of Mulberry and its Impact on Silkworm Cocoon Characters

  • Dasappa D.M. Ram Rao (Central Sericultural Research and Training Institute) ;
  • Ramaswamy S.N. (Central Sericultural Research and Training Institute)
  • 발행 : 2006.09.30

초록

An experiment was conducted to study the efficiency of cyanobacterial biofertilizer (CBB) with chemical (NPK) fertilizer on quantitative and qualitative characters of mulberry variety Kanva-2. Their influences on silkworm growth and cocoon characters were also studied. Ten different CBB and NPK fertilizer treatments were given to 5000 plants of established mulberry garden. Treatments were of four types viz., (i) T1 to T7: single and combination dose of CBB+50% NPK (ii) T8: combination dose of CBB + 25%NPK, (iii) T9: CBB only and (iv) T10: control-l00% NPK. Soil pH decreased and nutrients status increased in CBB (T1- T9) treated plots. Average of ten crops data on quantitative traits revealed that T7 (CBB [N. muscorum (1.0 g), A. variahilis (1.0) and S. millei (1.0 g)] + 50% NPK) was very effective in improving growth parameters. Leaf yield was also found high in treatment T7 (32.12 tons/ha/yr.) followed by T10 (31.17 tons/ha/yr.) and T8 (27.67 tons/ha/yr.). Leaf quality characters were found high in T7 and low in T9. Most of the quality traits in T7 are on par with control no. The results revealed that reduction in the dose of chemical fertilizers in T7 did not affect the leaf yield and leaf quality traits of mulberry. This clearly indicates that the efficiency of CBB (T7) provides nitrogen, increases essential nutrients available in soil, maintain soil pH and supply growth substances required for the improvement of leaf yield and leaf quality of mulberry. Bioassay study also revealed no significant difference in silkworm growth and cocoon characters between treatments T7 and T10. Economics calculated revealed that T7 is highly economical and beneficial over T10 by gaining an amount of Rs. 660/-/acre/crop. Thus, treatment T7 containing N. muscorum (1.0 g), A. variahilis (1.0 g) and S. millei (1.0 g) + 50% NPK fertilizers can be recommended to sericulturists mainly to reduce the use of NPK fertilizers, by saving 50% of its cost and to improve soil fertility conditions, which in turn improves leaf yield and quality of mulberry.

키워드

참고문헌

  1. Ambica, P. K. Das, R. S. Katiyar and P. C. Chaudhury (1994) The influence of VAM association on growth, yield and nutrient uptake in some mulberry genotypes (Morus spp.) Indian J. Seric. 33, 166-169
  2. Anonymous (1978) Algal Technology for Rice Research Bulle­tin NO.9 Indian Agricutural Research Institute, New Delhi, India
  3. Anonymous (1999) Approval norms on farm inputs and disin­fectants for Central Silk Board Units, Bangalore. pp.1-39
  4. Bergman, B., A. N. Rai, C. Johansson and E. Soderback (1992) Cyanobacterial plant symbiosis. Symbiosis. 14, 61-81
  5. Bongale, U. D. and Chaluvachari (1995) Evaluation of 8 mul­berry germplasm varieties by leaf biochemical and bioassay moulting studies. Sericologia. 35, 83-94
  6. Bose, P. C. and B. B. Bindroo (2001) A comparative biochem­ical study of seven promising mulberry (Morus alba L.) vari­eties under rainfed condition of subtropical region. Indian J Seric. 40, 171-173
  7. Bose, P. C. and S. K. Majumder (1999) Effect of Blue green algae on chemical and nutritional properties of salt-affected soil of mulberry garden, productivity of mulberry and its economics. Sericologia. 39, 459-465
  8. Das, P. K., P. C. Chaudhury, A. Ghosh, R. S. Katiyar, Y. Madhava Rao, V. B., Mathur and Mazumder (1994) Studies on the effect of Bacteria Biofertilizer in irrigated mulberry (Morus alba L.) Indian J. Seri. 33, 170-173
  9. Dubey, Y. P. (2000) Biofertilizers-A component of plant nutri­ent supply system. Farmer and Parliament. 37, 13-14
  10. Dubios, M. K., K. A. Giller, K. Hamilton, P. A. Reier and F. Smith (1956) Colorimetric methods for the determination of sugar and related substances. Ann. Chem. 28, 350-356 https://doi.org/10.1021/ac60111a017
  11. Ehiras, S. M., M. Ohmori and N. Sato (2003) Genome wide expression analysis of the responses to nitrogen deprivation in the heterocyst forming cyanobacteria Anabaena sp. Strain pcc 7120 DNA. Res. 10, 97-113
  12. Gangawar, S. K. and K. Thangavelu (1992) Evaluation ofbiof­ertilizers for establishment of mulberry (Morus alba L.) Sericologia. 32, 173-181
  13. Gantar, M., N. W. Kerby, P. Rowell and Z. Obreh, (1991) Col­onization of wheat (Triticum Vulgare L.) by $N_{2}$ fixing cyano­bacteria. I. A survey of soil cyanobacterial isolate forming association with roots. New Phytol. 118, 477-483 https://doi.org/10.1111/j.1469-8137.1991.tb00030.x
  14. Gantar, M., Peter Rowell, W. Nigel, Kerby and I. W. Suther­land (1995) Role of Extracellular Polysaccharide in the col­onization of wheat (Triticum Vulgare L.) roots by $N_{2}$-fixing cyanobacteria. Bio. Fertil Soil. 19, 41-48 https://doi.org/10.1007/BF00336345
  15. Hashem, M. A. (1997) Role of blue green algae in improving soil fertility and reclaiming salinity of soil Annual Report of BAURES, Financial Research Projects BAD: Mymensingh, India
  16. Hashem, M. A. (2001) Problems and prospects of cyanobacte­rial biofertilizers for rice cultivation. Aust. J. Plant Physiol. 28, 881-888
  17. Hemanth Kumar and B. D. Kaushik (1989) Bioameliorant blue green algae and salt affected soil. Proceeding of National Seminar on biofertilizer Technology Transfer Aurangabad, India. pp. 30
  18. Hiratsuka, E. (1971) Researches on the nutritional of the silk­worm. Shanghi Shikenjo. Hokoku Tech. Bull. 2, 353-412
  19. Hiscox, J. D. and H. F. Israelisiam (1979) A method for the extraction of chlorophyll from leaf tissue without macera­tion. Can. J. Bot. 57, 1332-1334 https://doi.org/10.1139/b79-163
  20. Horie, Y (1978) Quantitative requirements for growth of the silkworm, Bombyx mori L. J. A. R. Q. (Japan) 12, 211-217
  21. Horie, Y. (1980) Recent advances in Sericulture. Annu. Rev. Entomol. 25, 49-71 https://doi.org/10.1146/annurev.en.25.010180.000405
  22. Huang, G., Qing Fan, Sigal Lechnoyossef, Elizabeth wojcuich, C. Peter Wolk, Takakazu Kaneko and Satoshi Tabata (2005) Clustered genes required for the synthesis of Heterocyst envelope polysaccharide in Anabaena sp. Strain pcc 7120. Journal of Bacteriology. 187, 1114-1123 https://doi.org/10.1128/JB.187.3.1114-1123.2005
  23. Islam and M. A. Hashem (1995) Role of blue green algae in improving soil environment. Bangladesh Journal of Training and Development. 8, 112-118
  24. Jacobson, B. L., Young Kee Chae, L. John, Markley, Ivan Raymut and M. H. Holden (1993) Molecular structure ofthe oxidized recombinant Heterocyst [2Fe-2S] Ferredoxin from Anabaena 7120 determined to 1.7-A Resolution Biochemistry 32, 6788-6793 https://doi.org/10.1021/bi00077a033
  25. Jackson, M. L. (1973) Nitrogen determination for soil and plant tissues In: Soil chemical analysis Prentice-Hall ofIndia (Ltd.). New Delhi. pp.183-204
  26. Jayaram, S. and S. Shanmugasundaran (1993) Algal biofertil­izer choice species of Scytonema. Proc. Natt. Sem. On Cyanobacterial Research-Indian Science (ed.) G. Subrama­nian NMFC, BARD Tiruchirapalli pp.24-31
  27. Krishnaswamy, S. (1978) New technology of Silkworm rear­ing. Bulletin, CSRI 2, 1-24
  28. Lowry, O. H., N. J. Rosenbrough, A. L. Flair and R. C. Randal (1951) Protein measurement with folin-phenol reagent. J. Bio. Chem. 93, 265-275
  29. Machii, H. (1989) Varietal differences of nitrogen and amino acid contents in mulberry leaves. Acta Sericul. Entmol. 1, 51-61 I In Japanese
  30. Machii, H. and K. Katagiri (1991) Varietal differences in nutri­tive values of mulberry leaves for rearing silkworm J. A. R. Q. (Japan) 25, 202-208
  31. Mackerras, A. H., B. N. Youens, R. C. Weir, G. D. Smith, (1990) In cyanophycin involved in the integration of nitro­gen and carbon metabolism in the cyanobacteria Anabaena cylindrical and Gloecotheca grown on light/dark cycles. J. Gen. Microbiol. 136, 2049-2056 https://doi.org/10.1099/00221287-136-10-2049
  32. Marwaha, B. C. (1995) Biofertilizers-A supplementary source of plant nutrient. Fert. News. 40, 39-50
  33. Meeks, J. C. and J. Elahi (2002) Regulation of cellular differ­entiation in filamentous cyanobacteria in free living and plant associated symbiotic growth status. Microbiol Mol. BioI. Rev. 66, 94-121 https://doi.org/10.1128/MMBR.66.1.94-121.2002
  34. Paul, D. C., G. Subba Rao and D. C. Deb (1992) Impact of dietary moisture on nutritional indices and growth of Bom­byx mori and concomitant larval duration. J. Insect Physiol. 38, 229 https://doi.org/10.1016/0022-1910(92)90071-K
  35. Peters, G. A. (1990) Azolla and Plant cyanobacteria symbiosis: as pests of form and function In: Polsinelli M. Materassi R. Vincenzini M. (eds.) Nitrogen fixation, Proceedings of the 5th International symbiosis on nitrogen fixation with non­legumes. Kluwer Academic Dordrecht pp. 377-388
  36. Rai, A. N. (ed.) (1990) A Hand book of symbiotic cyanobacte­ria, CRC Press. Boca Raton-In Plant biochemistry and Molecular biology-Peter Lea and Richard C. Leegood. pp 129-153
  37. Rai, A. N., C. K. Derba and B. Bergman (2000) Cyanobacte­rium plant symbiosis REVIEW. New Phytol. 147, 449-481 https://doi.org/10.1046/j.1469-8137.2000.00720.x
  38. Reddy, M. P., D. M. Ram Rao, R. S. Verma, B. Srinath and R. S. Katiyar (2000) Effect ofVAM inoculated and addition of phosphorus on the growth of S-13 mulberry saplings. Indian J. Seric. 39, 12-15
  39. Rowell, P. and N. W. Kerby (1991) Cyanobacteria and their symbionts. In: Biology and Biochemistry of nitrogen fixation (M.J. Dilworth and A.R. Glenn eds) Elsevier. New York. 373-407
  40. Roy Chodhury, P. Kaushik, B. D. Krishnamurthy and G. S. Venkataraman (1979) Effect of Blue green algae and Azolla application on the aggregation status of the soil. Cur. Sci. 48, 454-455
  41. Snedecor, G. W. and W. G. Cochran (1967) Statistical methods. Oxford and IBU Publishing Co. New Delhi, India
  42. Spies, J. R. (1955) Colorimetric procedure for amino acid and phenol. In. Methods In Enzymology colonick, S. P. and N. P. O. Kaplan (eds), pp. 461-477
  43. Stewart, W. D., P. Rowell and A. N. Rai (1983) Cyanobacteria-­Eukaryotic Plant Symbiosis. Ann. Microbia (Paris) 134, 205­-228
  44. Subba Rao, N. S. (1988) Biofertilizer-Potentialities and prob­lem. In: S. P. Sen and P. Polit (Eds). Biofertilizer potentiali­ties and problems. Plant Physiology forum Nayaprakash, Calcutta, 7-16
  45. Sujathamma, P. and S. B. Dandin (2000) Leaf quality evalua­tion of mulberry (Morus spp.) through chemical analysis Indian J. Seric. 39, 117-121
  46. Venkataraman, G. S. (1972) In: Algal biofertilizer and rice cul­tivation Today and Tomorrow Printers and Publishers, New Delhi 1-75
  47. Venkataraman, G. S. and B. Rajyalakshmi (1972) Relative tol­erance of blue green algae to pesticides. Indian J. Agri. Sci. 42, 119
  48. Venkataraman, L. V. (1986) Blue Green Algae as Biofertilizer In: CRe. Hand Book of Microalgal Mass Culture (ed). A. Richmond CRC Press. Boca Roton, Florida, 455-471
  49. Vijayan, K., A. Tikader, K. K. Dass, B. N. Roy and T. Pawan Kumar (1996) Genotypic influence on leaf moisture content of moisture retention capacity in mulberry (Morus spp.) Bull. Seric. Res. 7, 95-98
  50. Vijayan, K., M. K. Raghunath, K. K. Das, A. Tikader, S. P. Chakraborthi, B. N. Roy and S. M. H. Qadri (1997) Studies on leaf moisture of mulberry germplasm varieties. Ind. J. Seric. 36, 155-157