Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effect of Treatment of Lipopolysaccharides on Reproductive Performance in Lactating Dairy Cows

젖소에 있어서 Lipopolysaccharide의 처리가 번식 성적에 미치는 영향

  • Baek K.S. (National livestock Research institute, R. D. A.) ;
  • Park, S.B. (National livestock Research institute, R. D. A.) ;
  • Park, S.J. (National livestock Research institute, R. D. A.) ;
  • Kim, H.S. (National livestock Research institute, R. D. A.) ;
  • Lee, W.S. (National livestock Research institute, R. D. A.) ;
  • Jeong, G.Y. (National livestock Research institute, R. D. A.) ;
  • Lee, H.J. (National livestock Research institute, R. D. A.) ;
  • Hur, T.Y. (National livestock Research institute, R. D. A.) ;
  • Jeon B.S. (National livestock Research institute, R. D. A.) ;
  • Ahn B.S. (National livestock Research institute, R. D. A.) ;
  • Khan M. Aiumal (National livestock Research institute, R. D. A.)
  • Published : 2006.09.30
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Abstract

This study was carried out to investigate the effect of intra-uterine infusion of lipopolysaccharide (LPS) on reproductive performance in lactating dairy cows. Conception rate at first service was higher (56.0%) in dairy cows treated with LPS compared to non-treated cows (20.0 %). Cumulative conception rate by overall services (more than 2 services) after LPS treatment was also higher (64.0%) in dairy cows treated with LPS compared to non-treated cows (40.0%). Services per conception was significantly higher in control ($2.0{\pm}0.1$) compared with LPS ($1.2{\pm}0.4$) treated group (p<0.05).

본 연구는 젖소에 있어서 lipopolysaccharide의 처리가 번식 성적에 미치는 영향을 구명하기 위하여 2003년부터 2005년까지 3년간에 걸쳐 축산연구소 개방형 깔짚우사에서 사육중인 홀스타인 착유우 50두를 대상으로 처리구 및 대조구 각각 25두씩을 공시하였고 분만후 20일째에 1회에 한하여 Bacteroids helcogenes와 Fusobacterium varium으로부터 분리한 LPS $100{\mu}g$을 PBS 용액 35 ml에 희석하여 수정란 이식용 카테타로 자궁내에 주입한 후 발정 발현시 인공 수정을 실시하여 다음과 같은 결과를 얻었다. 1. LPS 처리후 첫 수정에 의한 수태율은 대조구 및 처리구가 각각 20.0% 및 56.0%로 처리구가 대조구에 비하여 높은 경향을 나타내었다. 2. LPS 처리후 2회 이상 수정에 의한 수태율은 대조구 및 처리구가 각각 40.0% 및 64.0%로 처리구가 대조구에 비하여 높은 경향을 나타내었다. 3. 수태된 개체들의 수태당 종부횟수는 대조구 및 처리구가 각각 $2.0{\pm}0.1$회 및 $1.2{\pm}0.4$회로 처리구가 대조구에 비하여 유의적으로 낮았다(p<0.05).

Keywords

References

  1. Ahlers D and Grunert E. 1993. Zur Problematik der Behandlung des infizierten Uterus beim Rind im Puerperium. Prakt. Tierarzt Sondert. Coll Vet. XXIV, pp. 57-62
  2. Ahmad R, Amin SM and Kazmi SE. 1985. Studies on the bacterial causes of delayed uterine involution in postpartum buffaloes. Pak. Vet. J., 5: 168-170
  3. Akhtar MH and Singh BK. 1979. Viability and fertility rate of spermatozoa in bovine cervical mucous under normal and disease conditions. Indian Vet. J., 56:112-117
  4. Correa MT, Erb H and Scarlett J. 1993. Path analysis for seven postpartum disorders of Holstein cows. J. Dairy Sci., 76:1305-1312 https://doi.org/10.3168/jds.S0022-0302(93)77461-5
  5. Dhaliwal GS, Murray RD and Woldehiwet Z. 15 September 2001. Some aspects of immunology of the bovine uterus related to treatments for endometritis. Anim. Reprod. Sci., 67: 135-152 https://doi.org/10.1016/S0378-4320(01)00124-5
  6. Dhaliwal GS, Singh J and Singh N. 1998. Treatment of repeat breeding in dairy cows-a comprehensive approach in: Proceedings of the 20th World Buiatrics Congress. Sydney. pp. 673-676
  7. Dohmen MJW, Huszenicza GY, Fodor M, Kulcsar M, Vamos M, Porkolab L, Szilagyi N and Lohuis JACM. 1996. Bacteriology and fertility in healthy postpartum cows and cows with acute endometritis. Proc. XIX world Buiatrics Congress. pp. 238-241
  8. Gupta RC, Simha AK and Krishnaswamy A. 1983. Studies on the efficacy of some post-service intrauterine infusions on the conception rate in repeat breeding cattle. Theriogenology, 20:559-564 https://doi.org/10.1016/0093-691X(83)90079-1
  9. Hill AW, Hibbit KG and Shears AL. 1976. Increased antibacterial activity against E. coli in serum after the induction of endotoxin tolerance. Infect. Immun., 14:257-265
  10. Husssain AM and Daniel RCW. 1992. Effects of intrauterine infusion of Escherichia coli endotoxin in normal cows and in cows with endometritis induced by experimental infection with Streptococcus agalactiae. Theriogenology, 37: 791-810 https://doi.org/10.1016/0093-691X(92)90042-P
  11. Jainudeen MR and Hafez ESE. 1993. Reproductive failure in females. In reproduction in farm animals. 6th ed. E.S.E. Hafez(ed). Philadelphia Lea & Febiger. pp. 261-286
  12. Kaneene JB and Miller R. 1995. Risk factors for metritis in Michigan dairy cattle using herd- and cow-based modeling approaches. Prev. Vet. Med., 23: 183-200 https://doi.org/10.1016/0167-5877(94)00438-O
  13. Khan N, Ahmad M, Ahmad R and Ahmad A. 1985. Post parturn uterine involution and causes of its delay. Proc 1st World Buffalo Congr. Cairo Egypt. p. 964
  14. Lafi SQ and Kaneene JB. 1988. Risk factors and associated economic effects of the repeat breeder syndrome in dairy cattle. Vet. Bull. (London), 58:891-903
  15. Lafi SQ and Kaneene JB. 1992. Epidemiological and economic study of the repeat breeder syndrome in Michigan dairy cattle. I. Epidemiological modeling. Prev. Vete. Med., 14:87-98 https://doi.org/10.1016/0167-5877(92)90087-V
  16. Laven RA and Peters AR. 1996. Bovine retained placenta: aetiology, pathogenesis and economic loss. Vet. Rec., 139:465-471 https://doi.org/10.1136/vr.139.19.465
  17. Mellado M and Reyes C. 1994. Associations between periparturient disorders and reproductive efficiency in Holstein cows in northern Mexico. Prev. Vet. Med., 19:203-212 https://doi.org/10.1016/0167-5877(94)90089-2
  18. Peter JH and Ball H. 1997. Late embryo and early fetal mortality in the cow. Animal Breeding Abstracts, 65:75-95
  19. Scanlan CM. 1988. Introduction to veterinary bacteriology. Department of veterinary microbiology and parasitology. Texas veterinary medical center, Texas A&M University, College station, Texas. p. 8
  20. Semambo DKN, Boyd IS, Taylor DJ, Ayliffe TR and Omran SN. 1992. Ultrasonographic study of early embryonic loss induced by Actinomyces pyogenes in cattle. Vet. Rec., 131:7-12 https://doi.org/10.1136/vr.131.1.7
  21. Singh J, Sidhu SS, Dhaliwal GS, Pangaonkar GR, Nanda AS and Grewal AS. 2000. Effectiveness of lipopolysaccharide as an intrauterine immunomodulator in curing bacterial endometritis in repeat breeding cross-bred cows. Anim. Reprod. Sci., 59: 159-166 https://doi.org/10.1016/S0378-4320(00)00144-5
  22. Targowski SP. 1984. Immunologically induced uterine inflammation. in: Proceedings of the 10th International Congress on Animal Reproduction and AI. Urbana, Champaign, VI. pp. 473-475
  23. Nakao T. 2006. 한국우병학회 제11차 학술대회 및 한. 일 합동 산업동물 심포지움. 소의 주요 생산성 저해질병에 대한 대책 : 고비유우의 번식장해의 특징과 그에 대한 대책. 한국우병학회지, 11:1-21
  24. 류병호, 박우열, 김희숙, 박종옥. 1991. Proteus vulgaris RH-90에서 추출하여 감마선 조사시킨 Lipopolysaccharide(LPS)의 항암 및 면역 활성에 미치는 영향. 한국생물공학회지, 6:45-54