Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A Comparison of the Anti-inflammatory Activity of Surfactin A, B, C, and D from Bacillus subtilis

  • Kim, Sung-Dae (Laboratory of Veterinary Physiology & Signaling, College of Veterinary Medicine, Kyungpook National University) ;
  • Cho, Jae-Youl (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Park, Hwa-Jin (College of Biomedical Science and Engineering, and the Regional Research Center, Inje University) ;
  • Lim, Chang-Ryul (College of Biomedical Science and Engineering, and the Regional Research Center, Inje University) ;
  • Lim, Jong-Hwan (College of Veterinary Medicine, Chungnam National University) ;
  • Yun, Hyo-In (College of Veterinary Medicine, Chungnam National University) ;
  • Park, Seung-Chun (Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Sang-Keun (College of Veterinary Medicine, Chungnam National University) ;
  • Rhee, Man-Hee (Laboratory of Veterinary Physiology & Signaling, College of Veterinary Medicine, Kyungpook National University)
  • Published : 2006.10.31
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Abstract

Natural surfactins are a mixture of isoforms that differ slightly in their physiological properties. In previous research, we obtained surfactin A, B, C, and D from the Bacillus subtilis complex BC1212. We found that surfactin C inhibited nitric oxide (NO)-production and suppressed the expression of pro-inflammatory cytokine mRNA, which was stimulated by $1{\mu}g/ml$ of lipopolysaccharide (LPS) in murine RAW264.7 cells. In order to compare the anti-inflammatory effects of surf actin isoforms, we examined the inhibition of LPS-induced NO production and the pro-inflammatory cytokine expression level. Surfactin C inhibited the LPS-induced NO production in murine macrophage RAW264.7 cells the most. In addition, surf actin C was superior to other surfactin's subtypes regarding inhibiting the expression of inducible nitric oxide synthase (iNOS) and monocyte chemoattractant protein 1 (MCP-1). Finally, the anti-inflammatory activity of surf actin C is the most potent, compared with surfactin A, B, and D.

Keywords

References

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