Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제32권5호
  • /
  • Pages.436-446
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  • 2006
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  • 2234-7550(pISSN)
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  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
권호 표지

상악동염 병소 부위에서 세균의 분리 동정 및 항생제 감수성에 대한 연구

ANTIBIOTIC SUSCEPTIBILITY OF BACTERIA ISOLATED FROM MAXILLARY SINUSITIS LESION

  • 최영옥 (조선대학교 치과대학 구강악안면외과학교실) ;
  • 김수관 (조선대학교 치과대학 구강악안면외과학교실) ;
  • 김학균 (조선대학교 치과대학 구강악안면외과학교실) ;
  • 김영종 (조선대학교 치과대학 구강악안면외과학교실) ;
  • 최동국 (조선대학교 치과대학 구강악안면외과학교실) ;
  • 김미광 (조선대학교 치과대학 구강생화학교실) ;
  • 박순낭 (조선대학교 치과대학 구강생화학교실) ;
  • 김민정 (조선대학교 치과대학 구강생화학교실) ;
  • 국중기 (조선대학교 치과대학 구강생화학교실)
  • Choi, Young-Og (Dept. of Oral & Maxillofacial Surgery, Oral Biology Research Institute, Chosun University) ;
  • Kim, Su-Gwan (Dept. of Oral & Maxillofacial Surgery, Oral Biology Research Institute, Chosun University) ;
  • Kim, Hak-Kyun (Dept. of Oral & Maxillofacial Surgery, Oral Biology Research Institute, Chosun University) ;
  • Kim, Yong-Jong (Dept. of Oral & Maxillofacial Surgery, Oral Biology Research Institute, Chosun University) ;
  • Choi, Dong-Kook (Dept. of Oral & Maxillofacial Surgery, Oral Biology Research Institute, Chosun University) ;
  • Kim, Mi-Kwang (Dept. Oral Biochemistry, College of Dentistry, Oral Biology Research Institute, Chosun University) ;
  • Park, Soon-Nang (Dept. Oral Biochemistry, College of Dentistry, Oral Biology Research Institute, Chosun University) ;
  • Kim, Min-Jung (Dept. Oral Biochemistry, College of Dentistry, Oral Biology Research Institute, Chosun University) ;
  • Kook, Joong-Ki (Dept. Oral Biochemistry, College of Dentistry, Oral Biology Research Institute, Chosun University)
  • 발행 : 2006.10.30
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초록

The purpose of this study was to isolate and identify the bacteria in chronic maxillary sinusitis (CMS) lesions from 3 patients and to determine the antimicrobial susceptibility of them against 10 antibiotics. One of them was odontogenic origin and the others were non-odontogenic origin. Pus samples were collected by needle aspiration from the lesions and examined by culture method. Bacterial culture was performed in three culture systems (anaerobic, CO2, and aerobic incubator). Identification of the bacteria was performed by 16S rRNA gene (16S rDNA) nucleotide sequencing method. To test the sensitivity of the bacteria isolated from the maxillary sinusitis lesions against seven antibiotics, penicillin G, amoxicillin, tetracycline, ciprofloxacin, cefuroxime, erythromycin, clindamycin, and vancomycin, minimum inhibitory concentration (MIC) was performed using broth dilution assay. Our data showed that enterobacteria such as Enterobacter aerogenes (30%), Klebsiella pneumoniae (25%), and Serratia marcescens (15%) were predominately isolated from the lesion of non-odontogenic CMS of senile patient (70 year old). Streptococcus spp. (40.3%), Actinomyces spp. (27.4%), P. nigrescens, M. micros, and P. anaerobius strains were isolated in the lesion of odontogenic CMS. In the lesion of non-odontogenic CMS, Streptococcus spp. (68.4%), Rothia spp. (13.2%), and Actinomyces sp. (10.5%) were isolated. The susceptibility pattern of 10 antibiotics was determined according to the host of the bacteria strains ratter than the kinds of bacterial species. Even though the number of CMS was limited as three, these results indicate that antibiotic susceptibility test must be accompanied with treatment of CMS. The combined treatment of two or more antibiotics is better than single antibiotic treatment in the presence of multidrug-resistant bacteria in the CMS lesions.

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참고문헌

  1. Jiang RS, Liang KL, Jang JW, Hsu CY: Bacteriology of endoscopically normal maxillary sinuses. J Laryngol Otol 1999;113:825-828
  2. Paju S, Bernstein JM, Haase EM, Scannapieco FA: Molecular analysis of bacterial flora associated with chronically inflamed maxillary sinuses. J Med Microbiol 2003;52:591-597 https://doi.org/10.1099/jmm.0.05062-0
  3. Brook I: Microbiology of acute and chronic maxillary sinusitis associated with an odontogenic origin. Laryngoscope 2005;115:823-825 https://doi.org/10.1097/01.MLG.0000157332.17291.FC
  4. Yoshiura K, Ban S, Hijiya T, Yuasa K, Miwa K, Ariji E, et al: Analysis of maxillary sinusitis using computed tomography. Dentomaxillofac Radiol 1993;22:86-92 https://doi.org/10.1259/dmfr.22.2.8375560
  5. Abrahams JJ, Glassberg RM: Dental disease: a frequently unrecognized cause of maxillary sinus abnormalities? Am J Roentgenol 1996;166:1219-1223 https://doi.org/10.2214/ajr.166.5.8615273
  6. Mehra P, Murad H: Maxillary sinus disease of odontogenic origin. Otolaryngol Clin North Am 2004;37:347-364 https://doi.org/10.1016/S0030-6665(03)00171-3
  7. Krieg NR: Identification of Procaryotes. In G. Garrity (ed.), Bergey's Manual of Systematic Bacteriology, Vol. 1, 2th ed. Springer Verlag, New York, U.S.A. 2001;33-38
  8. Kook JK, Kim MK, Seong JH, Kim DK, Kim BO, Park JC, et al: A new method for rapid screening of bacterial species- or subspeciesspecific DNA probes. FEMS Microbiol Lett 2003;219:121-127 https://doi.org/10.1016/S0378-1097(03)00021-1
  9. Kook JK, Sakamoto T, Nishi K, Kim MK, Seong JH, Son YN, et al: Detection of Tannerella forsythia and/or Prevotella intermedia might be useful for microbial predictive markers for the outcome of initial periodontal treatment in Koreans. Microbiol Immunol 2005;49:9-16. Erratum in: Microbiol Immunol 2005;49:295 https://doi.org/10.1111/j.1348-0421.2005.tb03634.x
  10. Lane DJ, Pace B, Olsen GJ, Stahl DA, Sogin ML, Pace NR: Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci USA 1985;82:6955-6959
  11. Marchesi JR, Sato T, Weightman AJ, Martin TA, Fry JC, Hiom SJ, et al: Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 1998;64(2):795-799. Erratum in: Appl Environ Microbiol 1998;64:2333
  12. Kroes I, Lepp PW, Relman DA: Bacterial diversity within the human subgingival crevice. Proc Natl Acad Sci USA 1999;96:14547-14552
  13. van Winkelhoff AJ, Herrera D, Oteo A, Sanz M: Antimicrobial profiles of periodontal pathogens isolated from periodontitis patients in The Netherlands and Spain. J Clin Periodontol 2005;32:893-898 https://doi.org/10.1111/j.1600-051X.2005.00782.x
  14. Murray PR, Jorgensen JH: Quantitative susceptibility test methods in major united states medical center. Antimicrobial agent and chemotherapy 1981;20:66-70 https://doi.org/10.1128/AAC.20.1.66
  15. National Committee for Clinical Laboratory standards: Method for dilution antimicrobial susceptibility testing of bacteria that grow aerobically, 5th ed. Approved standard M7-A5. National Committee for Clinical Laboratory standards, Wayne, Pa. 2000
  16. National Committee for Clinical Laboratory standards: Methods for Methodfor antimicrobial susceptibility testing of anaerobic bacteria. 5th ed. Approved standard M11-A5. National Committee for Clinical Laboratory standards, Wayne, Pa. 2001
  17. Iikubo M, Sasano T, Shoji N, Sakamoto M: Nonsurgical treatment for odontogenic maxillary sinusitis using irrigation through the root canal: preliminary case report. Tohoku J Exp Med 2002;197:47-53 https://doi.org/10.1620/tjem.197.47
  18. Shin JH, Shim JD, Kim HR, Sinn JB, Kook J-K, Lee JN: Rothia dentocariosa septicemia without endocarditis in a neonatal infant with meconium aspiration syndrome. J Clin Microbiol 2004;42:4891-4892 https://doi.org/10.1128/JCM.42.10.4891-4892.2004
  19. Schiff MJ, Kaplan MH: Rothia dentocariosa pneumonia in an immunocompromised patient. Lung 1987;165:279-282 https://doi.org/10.1007/BF02714444
  20. Douglas CW, Heath J, Hampton KK, Preston FE: Identity of viridans streptococci isolated from cases of infective endocarditis. J Med Microbiol 1993;39:179-182 https://doi.org/10.1099/00222615-39-3-179
  21. MacKinnon MM, Amezaga MR, MacKinnon JR: A case of Rothia dentocariosa endophthalmitis. Eur J Clin Microbiol Infect Dis 2001;20:756-757 https://doi.org/10.1007/s100960100589
  22. Wallet F, Perez T, Roussel-Delvallez M, Wallaert B, Courcol R: Rothia dentocariosa: two new cases of pneumonia revealing lung cancer. Scand J Infect 1997;29:419-420 https://doi.org/10.3109/00365549709011841
  23. Helovuo H, Forssell K, Hakkarainen K: Oral mucosal soft tissue necrosis caused by superinfection. Report of three cases. Oral Surg Oral Med Oral Pathol 1991;71:543-548 https://doi.org/10.1016/0030-4220(91)90358-J
  24. Bernier S, Clermont S, Maranda G, Turcotte JY: Osteomyelitis of the jaws. J Can Dent Assoc 1995;61:441-442, 445-448
  25. Reiss I, Borkhardt A, Fussle R, Sziegoleit A, Gortner L: Disinfectant contaminated with Klebsiella oxytoca as a source of sepsis in babies. Lancet 2000;356:310
  26. Waxman DJ, Strominger JL: Penicillin-binding proteins and the mechanism of action of beta-lactam antibiotics. Annu Rev Biochem 1983;52:825-869 https://doi.org/10.1146/annurev.bi.52.070183.004141
  27. Kolokotronis A: Beta-lactamases producing anaerobic bacteria in dentoalveolar abscesses. J Oral Sci 1999;41:187-190 https://doi.org/10.2334/josnusd.41.187
  28. Hakenbeck R, Kaminski K, Konig A, van der Linden M, Paik J, Reichmann P, et al: Penicillin-binding proteins in beta-lactam-resistant streptococcus pneumoniae. Microb Drug Resist 1999;5:91-99 https://doi.org/10.1089/mdr.1999.5.91
  29. Wang X, Minasov G, Shoichet BK: The structural bases of antibiotic resistance in the clinically derived mutant beta-lactamases TEM-30, TEM-32, and TEM-34. J Biol Chem 2002;277:32149-32156 https://doi.org/10.1074/jbc.M204212200
  30. Belaaouaj A, Lapoumeroulie C, Canica MM, Vedel G, Nevot P, Krishnamoorthy R, et al: Nucleotide sequences of the genes coding for the TEM-like beta-lactamases IRT-1 and IRT-2 (formerly called TRI-1 and TRI-2). FEMS Microbiol Lett 1994;120:75-80
  31. Bascones Martinez A, Aguirre Urizar JM, Bermejo Fenoll A, Blanco Carrion A, Gay-Escoda C, Gonzalez-Moles MA, et al: Consensus statement on antimicrobial treatment of odontogenic bacterial infections. Med Oral Patol Oral Cir Bucal 2004;9:369-376
  32. Rodriguez-Avial I, Rodriguez-Avial C, Culebras E, Benitez A, Picazo JJ: Distribution of mef(A) and erm(B) genes in macrolide-resistant blood isolates of viridans group streptococci. J Antimicrob Chemother 2001;47:727-728 https://doi.org/10.1093/jac/47.5.727
  33. Jenssen WD, Thakker-Varia S, Dubin DT, Weinstein MP: Prevalence of macrolides-lincosamides-streptogramin B resistance and erm gene classes among clinical strains of staphylococci and streptococci. Antimicrob Agents Chemother 1987;31:883-888 https://doi.org/10.1128/AAC.31.6.883
  34. Grossman RF: The role of fluoroquinolones in respiratory tract infections. J Antimicrob Chemother 1997;40:59-62
  35. Cohen MA, Embil JM, Canosa T: Osteomyelitis of the maxilla caused by methicillin-resistant Staphylococcus aureus. J Oral Maxillofac Surg 2003; 61:387-390 https://doi.org/10.1053/joms.2003.50076
  36. Griffiths GK, VandenBurg MJ, Wight LJ, Gudgeon AC, Kelsey M: Efficacy and tolerability of cefuroxime axetil in patients with upper respiratory tract infections. Curr Med Res Opin 1987;10:555-561 https://doi.org/10.1185/03007998709108965
  37. Stoeckel K, Harell M, Dan M: Penetration of cefetamet pivoxil and cefuroxime axetil into the maxillary sinus mucosa at steady state. Antimicrob Agents Chemother 1996;40:780-783
  38. Choi JH, Seong JH, Kook JK, Kim DK: Comparison of cultural method and PCR method for the assay of tetracycline-resistant Fusobacterium nucleatum isolated in Korean. J Korean Acad Dent Health 2003;27:581-589