Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제32권5호
  • /
  • Pages.426-429
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  • 2006
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  • 2234-7550(pISSN)
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  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
권호 표지

치주인대 기원의 섬유아 세포에 압축력을 가한 경우 Interleukin-6 및 Interleukin-8의 발현 변화에 관한 연구

THE CHANGE OF EXPRESSION OF INTERLEUKIN-6 AND -8 AFTER THE APPLICATION OF THE STATIC COMPRESSIVE PRESSURE ON THE FIBROBLAST ORIGINATED FROM THE PERIODONTAL LIGAMENTS

  • 이연희 (서울대학교 치과대학 치과교정과) ;
  • 김성곤 (한림대학교 성심병원 구강악안면외과) ;
  • 남동석 (서울대학교 치과대학 치과교정과)
  • Lee, Yeon-Hee (Dept. of Orthodontics, College of Dentistry, Seoul National University) ;
  • Kim, Seong-Gon (Dept. of Oral and Maxillofacial Surgery, College of Medicine, Hallym University) ;
  • Nahm, Dong-Seok (Dept. of Orthodontics, College of Dentistry, Seoul National University)
  • 발행 : 2006.10.30
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초록

The fibroblast in the periodontal ligaments received various stress. Among them, compression and tension are quite important and they are related to the remodeling of tooth and alveolar bone. We studied the change of expression of interleukin-6 (IL-6) and interleukin-8 (IL-8) in the fibroblasts of the periodontal ligaments by real-time RT-PCR and ELISA. In results, the relative activity of IL-6 mRNA in 2 hours after was 1.54${\pm}$0.08 and 1.00${\pm}$0.05 in control and test, respectively (P<0.05). Its 12 hours after was 1.23${\pm}$0.06 and 2.78${\pm}$0.14 in control and test, respectively (P<0.05). The relative activity of IL-8 mRNA in 2 hours after was 1.00${\pm}$0.05 and 0.24${\pm}$0.01 in control and test, respectively (P<0.05). Its 12 hours after was 1.23${\pm}$0.06 and 0.63${\pm}$0.03 in control and test, respectively (P<0.05). The concentration of IL-6 was 1.02${\pm}$0.16 ng/ml, 0.90${\pm}$0.14 ng/ml, and 1.32${\pm}$0.12 ng/ml (P<0.05) in control, 2, and 12 hours after, respectively. The concentration of IL-8 was 2.26${\pm}$0.17 ng/ml, 1.70${\pm}$0.26 ng/ml (P<0.05), and 0.84${\pm}$0.47 ng/ml (P<0.05) in control, 2, and 12 hours after, respectively. In conclusion, the expression of IL-6 was significantly increased after the application of the static compressive force, but IL-8 was significantly decreased. Considering their known function, their expression is quite important in tooth and bone resorption.

키워드

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