Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제32권3호
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  • Pages.272-278
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  • 2006
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  • 2234-7550(pISSN)
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  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
권호 표지

인간 치주인대 유래 섬유모세포에 대한 흡수성 교원질 차폐막의 생체적합성

BIOCOMPATIBILITY OF ABSORBABLE COLLAGEN MEMBRANES IN HUMAN PDL-DERIVED FIBROBLASTS IN VITRO

  • 권용대 (경희대학교 치과대학 구강악안면외과) ;
  • 이백수 (경희대학교 치과대학 구강악안면외과) ;
  • 주성숙 (경희대학교 치과대학 구강해부학교실)
  • Kwon, Yong-Dae (Dept. of Oral & Maxillofacial Surgery, School of Dentistry, Kyung-Hee University) ;
  • Lee, Baek-Soo (Dept. of Oral & Maxillofacial Surgery, School of Dentistry, Kyung-Hee University) ;
  • Jue, Sung-Sook (Dept. of Oral Anatomy, School of Dentistry, Kyung-Hee University)
  • 발행 : 2006.06.30
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초록

Purpose: This study is designed to evaluate biocompatibility of three types of absorbable collagen GBR membrane in vitro. Material and Method: The human PDL fibroblasts culture was obtained through typical way and the cells used in the experiment was forth passage. The membranes examined were Experimental group A, B, C. All the 3-experimental groups were made of bovine pericardium and the membranes were excised into 5$\times$5mm respectively. The samples of the membranes were fixed on the 24-well plate with the double-sided adhesive tape. Then, 2ml of cell suspension which included $2{\times}10^4$cells was inoculated into the 24-well plate, and the cells were cultured for 1 week. Cellular viability and the alkaline phosphatase activity were measured with ELISA. The membranes in the culture were processed to examine with SEM. Results: The survival rate was highest in control and Experimental group A is the next, group B and group C in order of the value. The values are analyzed for statistical difference using Wilcoxon test. All the values of experimental groups are significantly lower than those of control, and the vaules among the experimental groups significantly differ from each other. Alkaline phosphatase level was identical order with the viable cell rate. SEM examination revealed that the PDL fibroblasts adherent on culture dish (control) and group A were spindle-shaped, but on group B and C, the cells were round-shaped without processes.

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