Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Use of ISSR Marker for the Variant Identification in Cornus kousa Buerg.

산딸나무 변이개체 선발을 위한 ISSR marker 이용

  • Published : 2006.08.30
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Abstract

For the variant identification of Korean dogwood, Cornus kousa Buerg., we investigated useful genetic marker using the ISSR primer. The overall amplicons was 58 in six primers and the mean number of amplicons per primer was 9.67. The UPGMA dendrogram based on the genetic distance showed two major clusters composed of pink-bracted and white-bracted groups respectively. And white-bracted group was divided to two groups, island and inland groups. This result showed that ISSR analysis provides useful markers for the variant identification even in the seedling or young tree of Cornus kousa, especially pink-bracted individuals of Isl. Oenaro.

외나로도 식물상을 조사하던 중 분홍색 포를 가진 산딸나무 개체를 발견하였다. 흰색 포를 가지는 일반 산딸나무 개체와 식별가능한 유전자 marker를 탐색하기 위하여 ISSR primer를 사용하였다. 50개의 primer 중 6개 primer에서 총 58개의 증폭산물을 관찰할 수 있었으며, primer당 평균 9.67개가 증폭되었다. UPGMA 방법에 의한 유집분석을 수행한 결과, 산딸나무 개체들은 포가 분홍색인 개체들과 흰색인 개체들이 따로 유집되었으며, 흰색인 개체들은 도서지역 개체들과 내륙지역 개체들로 유집되었다. 산딸나무 화색의 변이에 따른 유전적 다양성조사 결과, 특정 ISSR primer는 꽃이 없는 상태에서 변이 개체를 구분할 수 있는 유용한 marker로 사용될 수 있는 것으로 사려되었다.

Keywords

References

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