Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Effects of Short-term Treatment of Daidzein, Puerarin, Genistein and Tumerone on Mucin Secretion from Cultured Airway Epithelial Cells

  • Heo, Ho-Jin (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Lee, Hyun-Jae (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Kim, Cheol-Su (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Choi, Jae-Sue (Faculty of Food Science and Biothechnology, Pukyong National University) ;
  • Lee, Jung-Joon (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Young-Sik (Department of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
  • Kang, Sam-Sik (Department of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
  • Kim, Yun-Hee (Department of Oriental Medicine, College of Oriental Medicine, Daejeon University) ;
  • Park, Yang-Chun (Department of Oriental Medicine, College of Oriental Medicine, Daejeon University) ;
  • Seok, Jeong-Ho (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Lee, Choong-Jae (Department of Pharmacology, College of Medicine, Chungnam National University)
  • Published : 2006.09.25
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Abstract

In this study, we investigated whether daidzein, puerarin, genistein and (+)-ar-tumerone affect mucin secretion from cultured airway epithelial cells and compared with the inhibitory action of poly-L-lysine (PLL) and the stimulatory action of adenosine triphosphate (ATP) on mucin secretion. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled using $^3H$-glucosamine for 24 h and chased for 30 min in the presence of varying concentrations of each agent to assess the effects on $^3H$-mucin secretion. The results were as follows: daidzein, puerarin, genistein and (+)-ar-tumerone did not affect mucin secretion at the highest concentrations $(10^{-3}M)$, during 30 min of treatment period. Basically, this finding suggests that daidzein, puerarin and genistein - 3 components derived from Puerariae Radix - and (+)-ar-tumerone derived from Curcumae Rhizoma might not function as a mucoregulator in various inflammatory respiratory diseases showing mucus hypersecretion, although further studies are needed.

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References

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