Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Effects of Static Magnetic Fields on Phagocytic Activity of Murine Peritoneal Macrophages

  • Eun, Jae-Soon (College of Pharmacy, Woosuk University) ;
  • Ko, Dae-Woong (College of Pharmacy, Woosuk University) ;
  • Jeon, Yong-Keun (College of Pharmacy, Woosuk University) ;
  • Lee, Kyung-A (College of Pharmacy, Woosuk University) ;
  • Park, Hoon (College of Pharmacy, Woosuk University) ;
  • Ma, Tian-Ze (Department of Pharmacology, Chonbuk National University Medical School) ;
  • Kim, Min-Gul (Department of Pharmacology, Chonbuk National University Medical School) ;
  • Kwak, Yong-Geun (Department of Pharmacology, Chonbuk National University Medical School)
  • Published : 2006.09.25
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Abstract

Electro-magnetic fields and static magnetic fields generated from diverse home/environmental sources have been reported that these could make harmful effects on the human health such as suppression of immunity and tumorigenesis. However, the mechanisms for the biologic effects of electro-magnetic fields or static magnetic fields are still remained unclear. In this study, we examined the in vitro effects of static magnetic fields (SMF) on murine peritoneal macrophages. The cells were exposed in vitro to SMF of $150{\sim}250$ or $350{\sim}450$ G in 5% $CO_2$-incubator. The phagocytic activity of murine peritoneal macrophages was inhibited under exposure to SMF. In order to provide a more complete picture of molecular mechanism for the biological effect of SMF, we compared the levels of total proteins from macrophages with or without exposure to SMF using quantitative proteomic analysis. Proteins which were differentially expressed in macrophages exposed to SMF compared with non-exposed macrophages, were identified. Among them, the levels of trypsinogen 16, lactose-binding lectin Mac-2, galactoside-binding lectin, actin-like (Put. ${\beta}-actin$, vimentin) and electron transferring flavoprotein beta polypeptide were enhanced under exposure to SMF. These results suggest that SMF can affect the phagocytic activity of macrophages via diverse mechanisms.

Keywords

References

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