Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Screening of Medicinal Plants for Development of Functional Food Ingredients with Anti-Obesity

항비만 기능성 식품소재 개발을 위한 생약 추출물의 탐색

  • Kim, Yeong-Jin (Department of Microbiology and Immunology, College of Medicine, Pusan National University) ;
  • Kim, Bo-Hye (Department of Microbiology and Immunology, College of Medicine, Pusan National University) ;
  • Lee, Sun-Yi (Department of Microbiology and Immunology, College of Medicine, Pusan National University) ;
  • Kim, Min-Soo (Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Chan-Sun (Korea Research Institute of Bioscience and Biotechnology) ;
  • Rhee, Moon-Soo (Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Kang-Hyun (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Dong-Seob (School of Applied and Life Science, College of Natural Resources and Life Science, Pusan National University)
  • 김영진 (부산대학교 의과대학 미생물학교실 및 면역학교실) ;
  • 김보혜 (부산대학교 의과대학 미생물학교실 및 면역학교실) ;
  • 이선이 (부산대학교 의과대학 미생물학교실 및 면역학교실) ;
  • 김민수 (한국생명공학연구원) ;
  • 박찬선 (한국생명공학연구원) ;
  • 이문수 (한국생명공학연구원) ;
  • 이강현 (한국생명공학연구원) ;
  • 김동섭 (부산대학교 생명자원과학대학 생명응용과학부)
  • Published : 2006.09.30
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Abstract

In order to search anti-obesity agents, the methanol extracts of 155 herbal medicines were screened using with pancreatic lipase, which is involved in conversion of triglycerol to fatty acid. Among the tested medicinal plants, methanol extracts of Amsonia elliptica, Arecae pericarpium, Biota orientalis, Cinnamomum cassia, Curcuma aromatia, Elsholtzia ciliate, Glycyrrhiza uralensis, Mucunae Caulis, Rhus javanica, and Rubus coreanus showed potent inhibition at final concentration of $200\;{\mu}g/ml$ on pancreatic lipase activity. All of them were extracted into chloroform fraction. The relative inhibitory activities against pancreatic lipase by orlistat, the chloroform fraction of Arecae pericarpium and Cinnamomum cassia were 89, 80 and 80%, respectively. These results demonstrated that the screened medicinal plants could be develop as the effective lipase inhibitors in preventing and ameliorating obesity of human beings.

효과적인 항비만효과를 가진 식품소재 개발을 위해 155종의 생약추출물에 대해 pancreatic lipasae에 대한 억제효과를 탐색하였다. 탐색 결과, 정향, 대복피, 백자인, 계피, 강황, 향유, 감초, 오배자 그리고 복분자의 메탄올 추출물들이 $200\;{\mu}g/ml$ 농도에서 췌장지방분해효소에 대한 억제효과를 나타내었다. 특히, 상기의 추출물에 대해 추가적인 클로로포름 추출과정을 통해 얻은 분획에 있어 대복피와 계피의 경우, 현재 비만치료제로 시판되고 있는 orlistat와 비교하여 pancreatic lipase에 대해 80%와 89% 정도로 유사한 억제효과를 나타내었다. 이러한 결과는 생약추출물이 비만을 예방하는데 있어 효과적인 지방분해효소억제제로서 개발될 수 있음을 증명하는 것이다.

Keywords

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