Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Study on Identification and Purification of Germanium-fortified Yeast

게르마늄강화효모의 게르마늄결합 단백질의 분리 및 확인에 관한 연구

  • 이성희 (게란티제약(주) 중앙연구소) ;
  • 이상광 (한국기초과학지원연구원) ;
  • 이현주 (한경대학교 영양조리학과) ;
  • 이용섭 (서울벤처정보대학원대학교 컴퓨터응용기술학과) ;
  • 박은우 (서울대학교 응용생명화학부)
  • Published : 2006.03.31
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Abstract

This study was designed to investigate the optimum manufacturing condition of germanium-fortified yeast, and the binding properties of germanium (Ge) in germanium-fortified yeast. The nutritional optimum conditions were glucose 3.0 (w/v) %, yeast extracts 0.3 (w/v) % and peptone 0.5 (w/v) %, and the amounts of yeast cells were 67.4 mg/ml. And, the standard germanium-fortified yeast was produced under the condition at the ratio of yeast cell and germanium solution was 1 : 0.5 (50%), pH 6.5 and $35-40^{\circ}C$ during fermentation. In results of the identification, binding of germanium-protein showed structural difference between the inorganic Ge $(GeO_2)$ added during fermentation process and germanium-fortified yeast. Therefore, germanium-fortified yeast made by biosynthetic technology formed structurally safe organic germanium during fermentation process. Germanium-fortified yeast can be applied as a new functional material far the improvement of health, the prevention and treatment of chronic degenerative disease like cancer, and the enforcement of immune system.

본 연구는 게르마늄 강화 효모의 제조 공정을 위한 최적의 조건을 확인하고 제조된 게르마늄 강화 호모 내의 게르마늄 결합 상태 확인을 목적으로 수행하였다. 그 결과 영양소별 최적조건은 글루코스 3.0%, 효모추출물 0.3%, 펩톤 0.5%이었으며, 이 때 생성된 균체량은 67.4 mg/ml이었다. 또한 균체와 게르마늄 용액 혼합 비율은 1 : 0.5(50%), pH는 6.5 및 온도는 $35^{\circ}C$로 배양하는 조건이 높은 함량의 게르마늄을 효모 균체 내로 흡수시켜 게르마늄 강화 효모를 생산하는 것을 가능하게 하였다. 배양 과정을 통해 생산된 게르마늄 강화 효모는 배양 과정동안의 구조적 변화에 의해 효모 내에 흡수된 무기 형태인 $GeO_2$ 게르마늄과는 다른 구조를 나타내었다. 게르마늄 강화 효모는 효모 배양 과정을 통해 인체에 안전한 형태인 천연 유기 게르마늄을 형성하였다. 이는 각종 암, 성인병의 예방과 치료, 인체 면역력의 증진 등 건강 증진을 위한 새로운 기능성 원료로의 활용이 기대되며, 이에 대한 안전성 등의 지속적인 연구가 필요할 것으로 사료된다.

Keywords

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