Anti-allergic Activity of Oriental Medicinal Herbs

  • 발행 : 2006.10.01

초록

The effects of extracts from various Oriental medicinal herbs on mast-cell-mediated allergic reactions were investigated in this study. Out of these extracts, the medicinal herb Atractylodis rhizoma alba exhibited the most potent activity in the cells, with $IC_50$ values of $20.5\;{\mu}g/ml$, by DNP-BSA and $33.04\;{\mu}g/ml$, by compound 48/80. Similar to the in vitro activity, Atractylodis rhizoma alba inhibited compound-48/80-induced systemic anaphylaxis by 31.3% at 300 mg/kg in mice. It also suppressed the secretion of $TNF-{\alpha}$, a major pro-inflammatory cytokine in the cells. These results may suggest that Atractylodis rhizoma alba shows anti-allergic activity in compound 48/80-induced anaphylactic mice through the inhibition of mast cells.

키워드

참고문헌

  1. Alfonso A, Cabado AG, Vieytes MR, Botana LM (2000) Functional compartments in rat mast cells for camp and calcium on histamine release. Cell. Signal. 12:343-350 https://doi.org/10.1016/S0898-6568(00)00070-X
  2. Ali H, Cunha-Melo JR, Saul WF, Beaven MA (1990) Activation of phospholipase C via adenosine receptors provides synergistic signals for secretion in antigen-stimulated RBL-2H3 cells. Evidence for a novel adenosine receptor. J. BioI. Chem. 265:745-753
  3. Baumgartner RA, Beaven MA (1996) In Handbook of Experimental Immunology, 5th Ed. (Herzenbergm L.A., Herzenberg, L., Weir, D.M., and Blackwell, C., eds) Vol. 4, pp. 213.1-213.8, Blackwell Science, Inc., Cambridge, MA
  4. Chahdi A, Fraundorffer PF, Beaven MA (2000) Compound 48/80 activates mast cells phospholipase D via heterotrimeric GTP-binding proteins. J. Pharmacol. Exp. Ther. 292:122-130
  5. Galli SJ (1993) New concepts about the mast cells. N. Eng. J. Med. 328:257-265 https://doi.org/10.1056/NEJM199301283280408
  6. Gordon JR, Burd PR, Galli SJ (1990) Mast cells as a source of multifunctional cytokines. Immunol. Today 11 :458-464 https://doi.org/10.1016/0167-5699(90)90176-A
  7. Hikino H, Hikino Y, Yosioka I (1964) Studies on the constituents of Atractylodes. IX. Structure and autoxidation of atractylon. Chem. Pharm. Bull. (Tokyo). 12:755-760 https://doi.org/10.1248/cpb.12.755
  8. Hong S, Jeong H, Kim H (2003) Inhibitory effects of Xanthii fructus extract on mast cell-mediated allergic reaction in murine model. J. Ethnopharmacol. 88:229-234 https://doi.org/10.1016/S0378-8741(03)00232-0
  9. Kay AB (2001a) Allergy and allergic diseases. New Engl. J. Med. 344:30-37 https://doi.org/10.1056/NEJM200101043440106
  10. Kay AB (2001b) Allergy and allergic diseases. New Engl. J. Med. 344:109-113 https://doi.org/10.1056/NEJM200101113440206
  11. Kim CD, Lee W, No K, Park S, Lee M, Lim S, Roh S (2003) Anti-allergic action of buckwheat (Fagopyrum esculentum Moench) grain extract. Int. Immunopharmacol. 3:129-136 https://doi.org/10.1016/S1567-5769(02)00261-8
  12. Kim DK (2001) Pharmacognosy. Dongmyung-Sa, Seoul, p. 116-119
  13. Laemmli U K (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage t4. Nature 227:680-685 https://doi.org/10.1038/227680a0
  14. Lee JH, Chang SH, Park YS, Her E, Lee BY, Park JW, Han JW, Kim YM, Choi WS (2004a) In-vitro and in-vivo antiallergic actions of Arecae semen J. Pharm. Pharmacol. 56:927-933 https://doi.org/10.1211/0022357023808
  15. Lee JH, Seo JY, Ko NY, Chang SH, Her E, Park T, Lee BY, Han JW, Kim YM, Choi WS (2004b) Inhibitory activity of Chrysanthemi sibirici herba extract on RBL-2H3 cells mast cells and compound 48/80-induced anaphylaxis. J. Ethnopharmacol. 95:425-430 https://doi.org/10.1016/j.jep.2004.08.023
  16. Makino T, Furuta Y, Wakushima H, Fujii H, Saito K, Kano Y (2003) Anti-allergic effect of Perilla frutescens and its active constituents. Phytother. Res. 17:240-243 https://doi.org/10.1002/ptr.1115
  17. Mousli M, Bueb J-L, Brooner C, Rouot B, Landry Y (1990) G protein activation: A receptor-independent mode of action for cationic amphiphilic neuropeptides and venom peptides. Trends in Pharmacological Science 11:358-362 https://doi.org/10.1016/0165-6147(90)90179-C
  18. Nagai T, Arai Y, Emori M, Nunome S, Yabe T, Takeda T, Yamada H (2004) Anti-allergic activity of a Kampo (Japanese herbal) medicine 'Sho-seryu-to (Xiao-Qing-Long-Tang)' on airway inflammation in a mouse model. Int. Immunopharmacol. 4:1353-1365 https://doi.org/10.1016/j.intimp.2004.05.021
  19. Ogasawara T, Murakami M, Suzuki-Nishimura T, Uchida MK, Kudo I (1997) Mouse bone marrow-derived mast cells undergo exocytosis, prostanoid generation, and cytokine expression in response to G protein-activating polybasic compounds after coculture with fibroblasts in the presence of c-kit ligand. J. Immunol. 158:393-404
  20. Ozawa K, Szallasi Z, Kazanietz MG, Blumberg PM, Mischak H, Mushinski JF, Beaven MA (1993) Ca2+-Dependent and Ca2+-independent isozymes of protein kinase C mediate exocytosis in antigen-stimulated rat basophilic RBL-2H3 cells: Reconstitution of secretory responses with Ca2+ and purified isozymes in washed permeabilized cells. J. BioI. Chem. 268:1749-1756
  21. Rivera J (2002) Molecular adapters in FceRl signaling and the allergic response. Curr. Opin. Immunol. 14:688-693 https://doi.org/10.1016/S0952-7915(02)00396-5
  22. Senyshyn J, Baumgartner RA, Beaven MA (1998) Quercetin sensitizes RBL-2H3 cells to polybasic mast cell secretagogues through increased expression of Gi GTP-binding proteins linked to a phospholipase C signaling pathway. J. Immunol. 160:5136-5144
  23. Swieter M, Midura RJ, Nishikata H, Oliver C, Berenstein EH, Mergenhagen SE, Hascall VC, Siraganian RP (1993) Mouse 3T3 fibroblasts induce rat basophilic leukemia (RBL-2H3) cells to acquire responsiveness to compound 48/80. J. Immunol. 150:617-624
  24. Yi J, Hong S, Kim J, Kim H, Song H, Kim H (2002) Effect of Acanthopanax senticosus stem on mast cell-dependent anaphylaxis. J. Ethnopharmacol. 79:347-352 https://doi.org/10.1016/S0378-8741(01)00403-2