압출성형에 의해 제조된 구운 쇠고기 반응향의 특징적인 향기성분 동정

Identification of Characteristic Aroma-active Compounds from Burnt Beef Reaction Flavor Manufactured by Extrusion

  • 김기원 (단국대학교 식품공학과) ;
  • 서원호 (단국대학교 식품공학과) ;
  • 백형희 (단국대학교 식품공학과)
  • Kim, Ki-Won (Department of Food Engineering, Dankook University) ;
  • Seo, Won-Ho (Department of Food Engineering, Dankook University) ;
  • Baek, Hyung-Hee (Department of Food Engineering, Dankook University)
  • 발행 : 2006.10.01

초록

압출성형에 의해 제조된 구운 쇠고기 반응향의 향특성을 알아보기 위해 휘발성 성분 및 aroma-active화합물을 SDE-GC-MS-O법을 이용하여 분석하였다. HVP에 ribose, cysteine, furaneol, thiamin, methionine, 마늘 분말 및 인지질 등의 전구문질을 넣고 최적 압출성형 조건인 $160^{\circ}C$, 스크루 속도 45 rpm 및 윈료공급 속도 38 kg/hr에서 압출성형하였다. 압출성형에 의해 제조된 구운 쇠고기 반응향에서 모두 68개 의 휘발성 성분이 검출되었으며, 그 숫자는 furaneol을 빼고 압출성형 시킨 반응향과 HVP만 압출성형 시킨 시료에서 크게 줄어들었다. GC-O결과 구운 쇠고기 반응향에서 27개의 aroma-active 화합물이 검출되었으며, methional과 2-methyl-3-furanthiol이 가장 중요한 aroma-active화합물로 밝혀졌다. Furaneol이 구운 쇠고기 반응향 생성에 있어서 중요한 역할을 할 것으로 생각되어 진다.

To characterize aroma properties of burnt beef reaction flavor manufactured by extrusion, volatile flavor compounds and aroma-active compounds were analyzed by simultaneous steam distillation and solvent extraction (SDE)-gas chromatography-mass spectrometry-olfactometry (GC-MS-O). Hydrolyzed vegetable protein (HVP) was successfully extruded with precursors (glucose, cystine, furaneol, thiamin, methionine, garlic powder, and lecithin) at $160^{\circ}C$, screw speed of 45 rpm, and feed rate of 38 kg/hr. Sixty eight volatile flavor compounds were found in burnt beef reaction flavor. The number of volatile flavor compounds decreased significantly when HVP was extruded either with furaneol-free precursors or without precursors. Twenty seven aroma-active compounds were detected in burnt beef reaction flavor. Of these, methional and 2-methyl-3-furanthiol were the most intense aroma-active compounds. It was suggested that furaneol played an important role in the formation of burnt beef reaction flavor.

키워드

참고문헌

  1. Manley CH. Process flavor. 2nd ed, pp. 139-154. In: Source Book of Flavors. Reineccius GR (ed.) Chapman & Hall, New York. NY, USA (1994)
  2. Manley CH, Ahmedi S. The development of process flavors. Trends Food Sci. Technol. 6: 46-51 (1995) https://doi.org/10.1016/S0924-2244(00)88942-6
  3. Hurrell RF. Maillard reaction in flavor. pp. 399-423. In: Food Flavors, Part A. Introduction. Morton ID, MacLeod AJ (eds). Elsevier Scientific Publishing Compony. New York, USA (1982)
  4. Camire ME, Belbez EO, Flavor formation during extrusion cooking. Cereal Foods World 41:734-736 (1996)
  5. Izzo HV, Ho CT, Ammonia affects Maillard chemistry of an extruded autolyzed yeast extract: Pyrazine aroma generation and brown color formation. J. Food Sci. 57: 657-659 (1992) https://doi.org/10.1111/j.1365-2621.1992.tb08064.x
  6. Baek HH, Kim CJ, Ahn BH, Nam HS, Cadwallader KR. Aroma extract dilution analysis of a beeflike process flavor from extruded enzyme-hydrolyzed soybean protein. J. Agric. Food Chem. 49: 790-793 (2000) https://doi.org/10.1021/jf000609j
  7. Kim KW, Baek HH. Development of a burnt beef flavor by reaction flavor technology. Korean J. Food Sci. Technol. 35: 1045-1052 (2003)
  8. Umano K, Hagi Y, Nakahara K, Shyoji A, Shibamoto T. Volatile chemicals formed in the headspace of a heated D-glucose/L-cysteine Maillard model system. J. Agric. Food Chem. 43: 2212-2218 (1995) https://doi.org/10.1021/jf00056a046
  9. de Roos KB. Meat flavor generation from cysteine and sugars. pp. 139-154. In: Flavor Precursors: Thermal and Enzymatic Conversions. Teranishi R, Takeoka GR, Guntert M (eds). ACS Symposium Series 490, American Chemical Society, Washington DC, USA (1992)
  10. Mottram DS, Whitfield FB. Maillard-lipid interactions in nonaqueous systems: Volatiles from the reaction of' cysteine and ribose with phosphatidylcholine. J. Agric. Food Chem. 43: 1302-1306 (1995) https://doi.org/10.1021/jf00053a033
  11. Mottram DS, Whitfield FB. Volatile compounds from the reaction of cysteine, ribose, and phospholipid in low-moisture systems. J. Agric. Food Chem. 43: 984-988 (1995) https://doi.org/10.1021/jf00052a027
  12. Farmer LJ, Mottram DS, Whitfield FB. Volatile compounds produced in Maillard reactions involving cysteine, ribose and phospholipid. J. Sci. Food Agric. 49: 347-368 (1989) https://doi.org/10.1002/jsfa.2740490311
  13. Zhang Y, Ho CT. Formation of meatlike aroma compounds from thermal reaction of inosine 5-monophosphate with cysteine and glutathione. J. Agric. Food Chem. 39: 1145-1148 (1991) https://doi.org/10.1021/jf00006a031
  14. Parliment TH. Morello MJ, McGorrin RJ. Heterocyclic aroma compounds precursors. pp. 17-71. In: Chemistry of Heterocyclic Compounds in Flavors and Aromas. Vernin G (ed). Ellis Horwood Limited, West Sussex, England (1982)
  15. Whitfield FB, Mottram DS. Investigation of the reaction between 4-hydroxy-5-methyl-3(2H)-furanone and cysteine or hydrogen sulfide at pH 4.5. J. Agric. Food Chem. 25: 113-117 (1977) https://doi.org/10.1021/jf60209a049
  16. van den Ouweland GAM, Peer HG. Components contributing to beef flavor. Volatile compounds produced by the reaction of 4-hydroxy-5methyl-3(2H)-furanone and its thio analog with hydrogen sulfide. J. Agric. Food Chem. 23: 501-505 (1975) https://doi.org/10.1021/jf60199a045
  17. Bolton TA, Reineccius GA, Liardon R, Ba TH. Role of cysteine in the formation of 2-methyl-3-furanthiol in a thiamine-cysteine model system. pp. 17-71. In: Thermally Generated Flavors. Maillard, Microwave. and Extrusion Processes. Parliment TH. Morello MJ, McGorrin RJ (eds). ACS Symposium series 543, American Chemical Society. Washington DC, USA (1994)
  18. Guntert M. Bruning J, Emberger R, Hopper R, Kopel M. Surburg H, Werkhof P. Thermally degraded thiamin. A potent source of interesting flavor compounds. pp, 140-163. In: Flavor Precursors: Thermal and Enzymatic Conversions, Teranishi R, Takeoka GR, Guntert M (eds). ACS Symposium Series 490. American Chemical Society. Washington DC USA ( 1992)
  19. Whitfield FB. Mottram DS. Brock S, Puckey DJ, Salter LJ. Effect of phospholipid on the formation of volatile heterocyclic compounds in heated aqueous solutions of amino acids and ribose. J. Sci. Food Agric. 42: 261-272 (1988) https://doi.org/10.1002/jsfa.2740420309
  20. Shu CK, Mookherjee BD, Ho CT. Volatile components of the thermal degradation of 2,5-dimethyl-4-hydroxy-3(2H)-furanone. J. Agric. Food Chem. 33: 446-448 (1985) https://doi.org/10.1021/jf00063a030
  21. Hofmann T. Schieberle P. Evaluation of' the key odorants in a thermally treated solution of ribose and cysteine by aroma extract dilution techniques. J. Agric. Food Chem. 43: 2187-2194 (1995) https://doi.org/10.1021/jf00056a042
  22. van den Dool H, Kratz PD. A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. J. Chromatogr. 11 :463-471 (1963) https://doi.org/10.1016/S0021-9673(01)80947-X
  23. IOFI. Code of Practice. International Organization of Flavor Industries, Geneva, Switzerland (1990)
  24. Ames JM, MacLeod G. Volatile components of' a yeast extract composition. J. Food Sci. 50: 125-135 (1985) https://doi.org/10.1111/j.1365-2621.1985.tb13292.x
  25. Grosch W, Zeiler-Hilgart G. Formation of meat-like flavor compounds. pp. 183-192. In: Flavor Precursors: Thermal and Enzymatic Conversions. Teranishi R, Tekeoka GR, Guntert M (eds). ASC Symposium Series 490, American Chemical Society, Washington DC, USA (1992)
  26. Maga JA. Furans in food. CRC Crit. Rev. Food Sci. Nutr. 11: 355-400 (1979) https://doi.org/10.1080/10408397909527268
  27. Hirai C, Herz KO, Pokorny J, Chang SS, Isolation and identification of volatile flavor compounds in boiled beef J. Food Sci. 38: 393-397 (1973) https://doi.org/10.1111/j.1365-2621.1973.tb01438.x