Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Composition Analysis and Antioxidative Activity from Different Organs of Cirsium setidens Nakai

부위별 고려엉겅퀴(Cirsium setidens Nakai)의 이화학적 성상 및 항산화 활성 효과

  • Published : 2006.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

In the present study, we investigated the compositions including protein, lipid, ash, carbohydrate, and minerals as well as antioxidant activity of Cirsium setidens Nakai in order to detect the biological activities and develop novel functional resources. Different organs commonly had the highest concentration of potassium among 7 minerals evaluated in this study. The leaf had K at the concentration of 5371.97 mg/100 g, while the flower, and stem, the root and the boiled leaf at the concentration of 1770.62 mg/100 g, 1983 mg/100 g, 6096.74 mg/100 g and 1604.2 mg/100 g, respectively. The monosaccharides were composed of the xylose, galactose and glucose. The xylose was only detected in the flower and stem and the galactose was only detected in the stem. DPPH scavenging activity was measured at the $88.22\;{\mu}g/mL$ and $111.19\;{\mu}g/mL$ in root and leaf at $IC_{50}$ value in ethanol extracts, while $53.27\;{\mu}g/mL$, $75.84\;{\mu}g/mL$ and $257.48\;{\mu}g/mL$ in flower, boiled leaf and stem at $IC_{50}$ value, respectively, in water extracts. These results suggest that extracts from Cirsium setidens Nakai can be potentially used as novel resources for antioxidant and biological active substances.

고려엉겅퀴(Cirsium setidens Nakai)의 생리활성물질을 탐색하기위하여 고려엉겅퀴의 일반성분 분석과 항산화 활성을 조사하였다. 각 부위별추출물 중 탄수화물의 함량이 가장 높았으며, 그 밖에 성분은 조단백질, 수분, 조회분, 조지방 순으로 높게 나타났으며, 부위별 무기성분은 모두 일곱 종이 검사되었고, 칼륨의 함량이 가장 높았다. 부위별 K의 함량은 잎 부위에서 5,371.97 mg/100 g, 삶은 및 부위에서 1,770.62 mg/100 g, 꽃 부위는 1,983 mg/100 g, 줄기 부위는 6,096.74 mg/100 g, 뿌리 부위는 1,604.2 mg/100 g로 나타났다. 구성당은 xylose, galactose, glucose 총 3종이 함유되었으며 xylose는 꽃과 줄기부위에서만 분리, 동정되었고, galactose는 줄기 부위에서만, glucose는 전체 부위에서 분리, 동정되었다. 지방산 중 linolenic acid(C18:3)와 linoleic acid(C18:2)가 가장 많이 존재했다. linolenic acid(C18:3)는 잎, 삶은 잎, 꽃 부위에서 각각 41.63%, 34.01%, 18.83%로 가장 많이 나타났으며, linoleic acid(C18:2)는 뿌리, 줄기, 삶은 일 부위에서 각각 49.59, 40.72, 39.24%로 가장 많이 나타났다. 각 용매 별 추출 수율은 잎 부위의 물 추출물에서 27.22%, 삶은 잎 부위의 75% 메탄올과 물 추출물에서 14.2%, 꽃 부위의 75% 메탄올 추출물에서 17.85%, 줄기의 물 추출물에서 14.8%, 뿌리 부위의 75% 메탄올 추출물에서 36.64%로 가장 높은 수율을 보였다. 고려엉겅퀴의 부위별 항산화 효과에서 꽃의 물 추출물에서 $53.47\;{\mu}g/mL$, 삶은 잎의 물 추출물에서 $75.84\;{\mu}g/mL$, 뿌리의 에탄올 추출물에서 $88.22\;{\mu}g/mL$, 잎의 에탄올 추출물에서 $111.19\;{\mu}g/mL$L, 줄기의 물 추출물에서 $257.48\;{\mu}g/mL$의 순으로 높게 나타났다.

Keywords

References

  1. Lee SJ. Korean Folk Medicine. Seoul National University Press, Seoul. pp. 145-146 (1966)
  2. Ishida H, Umino T, Tsuji K, Kosuge T. Studies on antihemmorrhagic substance in Herbs classified as hemostatics in Chinese medicine. VII. On the antihmorrhagic principle in Cirsium japonicum DC. Chem. Pharm. Bull. 35: 861 (1987) https://doi.org/10.1248/cpb.35.861
  3. Yun HS, Chang IM. Separation and identification of Cirsimarin from Cirsium Pendulum Fisch. Korean J. Pharmacogn. 9: 145-147(1978)
  4. Park JC, Yu YB, Im SS, Lee JH. Isolation of flavone glycoside from the herb of Cirsium japonicum var. ussuriense. Korean J. Pharmacogn. 25: 96-97 (1994)
  5. Lee YC, Park YH. Chemical Studies on Cirsium Species (V). Chemical constituents of the roots of Cirsium xanthocanthum. Korean J. Pharmacogn. 15: 74-77 (1984)
  6. Do JC, Jung KY, Son KH. Isolation of pextolinarin from the aerial parts of Cirsium nipponicum. Korean J. Pharmacogn. 25: 73-75 (1994)
  7. Morita N, Shimizu M, Arisawa M. Two new flavone glycosides from Cirsium lineare. Phytochemistry. 12: 421-423 (1973) https://doi.org/10.1016/0031-9422(73)80032-9
  8. Rauen HM, Schriewer H. The antihepatotoxic effect of silymarin on liver damage in rats induced by carbon tetrachloride, d-galactosamine and allyl alcohol. Arzneimittelforsch 21: 1194-1201 (1971)
  9. Mourelle M, Murrel P, Favari L, Franco T. Prevention of $CCI_4$-induced cirrhosis by silymarin. Fund. Clin. Pharmacol. 3: 183-191 (1989) https://doi.org/10.1111/j.1472-8206.1989.tb00449.x
  10. Ingelman-sundberg M, Johansson I, Penttil K, Glaumann H, Lindros KO. Centrilobular expression of ethanol inducible cytochrome P450(IIEI) in rat liver. Biochem. Biophys. Res. Commun. 157: 55-60 (1988) https://doi.org/10.1016/S0006-291X(88)80010-X
  11. Ferenci P, Dragosises B, Dittrich H, Frand H, Benda L, Lochs H, Meryn S, Base W, Schneider B. Randomized controlled trial of silymarin treatment in patients with cirrhosis of the liver. J. Hepatol. 9: 105-113 (1989) https://doi.org/10.1016/0168-8278(89)90083-4
  12. Lim SS, Lee JH, Park JC. Isolation of flavone glycoside from Circium japonicum var ussuriense and biological activity on the cardiovascular system. J. Korean Soc. Food Sci. Nutr. 26: 242-247(1997)
  13. Wiseman H. Dietary influences on membrane function; importment in protection against oxidative damage and disease. Nutr. Biochem. 7: 2-6 (1996) https://doi.org/10.1016/0955-2863(95)00152-2
  14. Shin DH. The study course and movement of natural antioxidants. Korean Food Sci. Technol. 30: 14-18 (1997)
  15. Frankel EN. Antioxidants in lipid foods and their on food quality. Food Chem. 57: 51-54 (1996) https://doi.org/10.1016/0308-8146(96)00067-2
  16. AOAC. Official Methods of Analysis, 4th ed. Association of Official Analytical Chemist, Washington D.C. pp. 129-133 (1980)
  17. Bligh EG, Dyer WJ. A rapid methods of total lipid extraction and purification. Can. J. Biochem. Physil. 37: 911-917 (1959) https://doi.org/10.1139/o59-099
  18. Folch J, Lee SM, Stanley GH. A simple methods for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226: 497-509 (1957)
  19. Metcalfe LD, Schmitz AA. The rapid preparation of fatty acid esters for gas chromatographic analysis. Anal. Chem. 33: 363-364 (1961) https://doi.org/10.1021/ac60171a016
  20. Blakeney AB, Harris PJ, Henry RJ, Stone BA. A simple and rapid preparation of auditor acetates for monosaccharide analysis. Carbohyd. Res. 113: 291-299 (1983) https://doi.org/10.1016/0008-6215(83)88244-5
  21. Kumaran A, Karunakaran RJ. Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chem. 97: 109-114 (2006) https://doi.org/10.1016/j.foodchem.2005.03.032
  22. Lee HK, Kim JS, Kim NY, Kim MJ, Park SU, Yu CY. Antioxidant, antimutagenicity and anticancer activities of extracts from Cirsium japonicum var. ussuriense Kitamura. Korean J. Med. Crop Sci. 11: 53-61 (2003)