Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antimicrobial Activity of the Extract from Pyrola japonica against Bacillus subtilis

노루발풀(Pyrola japonica) 추출물의 Bacillus subtilis에 대한 항균활성

  • Park, Hae-Gun (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Cha, Mi-Ran (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Hwang, Ji-Hwan (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Kim, Ju-Young (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Mi-Suk (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Choi, Sun-Uk (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Hwang, Yong-Il (Department of Food Science and Biotechnology, Kyungnam University)
  • 박해건 (경남대학교 식품생명학과) ;
  • 차미란 (경남대학교 식품생명학과) ;
  • 황지환 (경남대학교 식품생명학과) ;
  • 김주영 (경남대학교 식품생명학과) ;
  • 박미숙 (경남대학교 식품생명학과) ;
  • 최선욱 (경남대학교 식품생명학과) ;
  • 박해룡 (경남대학교 식품생명학과) ;
  • 황용일 (경남대학교 식품생명학과)
  • Published : 2006.10.01
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Abstract

The antimicrobial substance from Pyrola japonica were extracted and isolated. Eighty percent ethanol extract of dried Pyrola japonica was fractionated to hexane, diethyl ether, ethyl acetate and aqueous layer. The hexane-soluble fraction showed the highest inhibitory activity against Bacillus subtilis. Moreover the hexane layer was fractionated into 5 groups by silica gel column chromatography. From the results, group No. 2 ($18{\sim}40$ fractions) showed the highest antimicrobial activity. The group was re-separated to 10 fractions by preparative thin layer chromatography and the peak I as active fraction was isolated by HPLC.

우리나라 전국 산지에서 쉽게 구할 수 있는 노루발풀을 건조시켜 80% ethanol 추출물과 여러 가지 용매 분획물의 미생물 증식 억제 효과에 대해서 검색하였다. 노루발풀의 80% ethanol 추출물은 그람 양성균 B. subtilis에서 농도 의존적으로 항균활성을 나타내는 반면 그람 음성균 E. coli에서는 활성이 나타나지 않았다. 노루발풀의 80% ethanol 추출물을 용매 분획하여 얻은 분획물 중 hexane층에서 항균활성이 나타나는 것을 확인하였다. 노루발풀의 항균활성 물질을 검색하기 위한 목적으로 hexane 분획물을 silica gel column chromatography, prep. TLC 및 HPLC를 실시하였으며 그 결과, peak I의 단일 물질이 노루발풀의 B. subtilis에 대한 항균활성을 나타내는 물질임을 확인할 수 있었다.

Keywords

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