생명과학회지 (Journal of Life Science)

  • 제16권6호
  • /
  • Pages.978-983
  • /
  • 2006
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

알코올성 간 손상에 대한 상황버섯 배양균사체 추출물의 효능

Effects of Mycelial Extract of Phellinus linteus on Ethanol-Induced Liver Injury in Rats

  • 최병태 (동의대학교 한의과대학 해부학교실) ;
  • 최영현 (동의대학교 한의과대학 생화학교실) ;
  • 길영기 (고신대학교 의과대학 해부학교실)
  • Choi, Byung-Tae (Departments of Anatomy, College of Oriental Medicine, Dong-eui University) ;
  • Choi, Yung-Hyun (Departments of Biochemistry, College of Oriental Medicine, Dong-eui University) ;
  • Gil, Young-Gi (Department of Anatomy, Medical School, Kosin University)
  • 발행 : 2006.10.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

상황버섯 배양균사체 추출물 (MCPL)이 알코올성 간 손상에 미치는 영향을 살펴보았다. Sprague-Dawley계 흰 쥐에 40% 알코올 3 ml을 MCPL (5 mg 및 15 mg/Kg)과 함께 1일 1회 10일간 투여하였다. 간 기능의 표지가 되는 혈청내 AST와 ALT값이 에탄올에 의해 현저히 증가하였으나 MCPL 투여에 의해 저하되며 특히 ALT 값이 유의성 있게 낮아졌다. 병리조직학적으로 살펴보면 알코올에 의해 염증세포의 침윤, 쿠퍼세포 반응 및 국소적 염증이 유발되나 MCPL투여에 의해 그 정도가 다소 완화되었다. 간소엽내 글리코겐 분포도 알코올에 의해 감소하나 MCPL투여에 의해 중심정맥주변 부위의 분포가 일부 회복되었다. 염증관련 단백질에 대한 Western blot 및 면역조직화학적 반응을 보면 에탄올 투여에 의해 COX-2, iNOS 및 $TNF-{\alpha}$ 면역반응이 증가하나 MCPL투여에 의해 발현의 감소를 볼 수 있었다. 이상의 결과로 보아 MCPL은 알코올에 의한 간 손상에 대해 보호 기능을 가짐을 알 수 있다.

We investigated the anti-inflammatory effects of mycelial culture extract from Phellinus linteus (MCPL) for suppression in the process of ethanol-induced inflammation in rat liver. Levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were significantly increased in the serum of ethanol-treated rats compared to normal. However, the level of ALT was arrested markedly in ethanol-treated rats with MCPL compared to ethanol alone treated control ones. Severe histopathological changes of liver such as cloudy swelling, inflammatory cells infiltration, Kupffer cell reaction and focal necrosis were demonstrated in the rats challenged with ethanol compared with normal. Fewer scores of these changes were observed in MCPL-treated rat with recovered glycogen in centrolobular region of hepatic lobule. The Western analysis showed that the expression of inflammatory proteins such as cyclooxygenase (COX)-1, COX-2, inducible nitric oxide synthase (iNOS), tumor necrosis factor $(TNF)-{\alpha}$ were increased in the ethanol-treated rat. But decline of COX-2 and iNOS expression were observed in MCPL-treated rat. Immunohistochemical analysis showed that the expression of COX-2 and $TNF-{\alpha}$ tended to increase in ethanol-treated rat, but decrease of these reactions were induced by MCPL treatment. These results suggest that MCPL may act as a protective agent for alcohol-induced liver injury through a regulating inflammation-related proteins.

키워드

참고문헌

  1. Ahn, S. M., J. H. Lee, Y. H. Choi, Y. T. Lee, K. T. Chung, Y. K. Jeong, U. N. Jo and B. T. Choi. 2006. Effects of fermented liquor by using mycelium of Phellinus linteus on the expression of inflammation-related proteins in human hepatoma cells and rat liver. Kor. J. Life Sci. 16, 113-119 https://doi.org/10.5352/JLS.2006.16.1.101
  2. Chen, G. and D. V. Goeddel. 2002. TNF-R1 signaling: a beautiful pathway. Science 296, 1634-1635 https://doi.org/10.1126/science.1071924
  3. Cheng, J., H. Imanishi, H. Iijima, S. Shimomura, T. Yamamoto, Y. Amuro, A. Kubota and T. Hada. 2002. Expression of cyclooxygenase 2 and cytosolic phospholipase A(2) in the liver tissue of patients with chronic hepatitis and liver cirrhosis. Hepatol. Res. 23, 185-195 https://doi.org/10.1016/S1386-6346(01)00177-2
  4. Choi, Y. H., M. K. Huh, C. H. Ryu, B. T. Choi and Y. K. Jeong. 2004. Induction of apoptotic cell death by mycelium extracts of Phellinus linteus in human neuroblastoma cells. Int. J. Mol. Med. 14, 227-232
  5. Dai, L. L., J. P. Gong, G. Q. Zuo, C. X. Wu, Y. J. Shi, X. H. Li, Y. Peng, W. Deng, S. W. Li and C. A. Liu. 2003. Synthesis of endotoxin receptor CD14 protein in Kupffer cells and its role in alcohol-induced liver disease. World J. Gastroenterol. 9, 622-626 https://doi.org/10.3748/wjg.v9.i3.622
  6. Han, S. B., C. W. Lee, Y. J. Jeon, N. D. Hong, I. D. Yoo, K. H. Yang and H. M. Kim. 1999. The inhibitory effect of polysaccharides isolated from Phellinus linteus on tumor growth and metastasis. Immunopharmacol. 41, 157-164 https://doi.org/10.1016/S0162-3109(98)00063-0
  7. Kim, H. M., S. B. Han, G. T. Oh, Y. H. Kim, D. H. Hong, N. D. Hong and I. D. Yoo. 1996. Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus. Int. J. Immunopharmacol. 18, 295-303 https://doi.org/10.1016/0192-0561(96)00028-8
  8. Kim, S. H., H. S. Lee, S. Lee, J. Cho, K. Ze, J. Sung and Y. C. Kim. 2004. Mycelial culture of Phellinus linteus protects primary cultured rat hepatocytes against hepatotoxins. J. Ethnopharm. 95, 367-372 https://doi.org/10.1016/j.jep.2004.08.007
  9. Kim, S., C. Y. Hwang, N. K. Kim, M. C. Park and J. Kim. 2002. Effects of cordyceps militaris on $CCl_4-induced$ liver damage and cancer cell $(HepG_2\;cell)$ growth. Kor. J. Oriental. Physol. Pathol. 16, 684-692
  10. Kramer, A. A., K. F. Salhab, A. E. Shafii, J. Norman, L. C. Carey and C. Mendez. 1999. Induction of tolerance to hemorrhagic or endotoxic shock involves activation of $NF-{\kappaB}$. J. Surg. Res. 83, 89-94 https://doi.org/10.1006/jsre.1999.5571
  11. Mann, R. E., R. G. Smart and R. Govoni. 2003. The epidemiology of alcoholic liver disease. Alcohol Res. Health. 27, 209-219
  12. Nagy, L. E. 2004. Molecular aspects of alcohol metabolism: transcription factors involved in early ethanol-induced liver injury. Annu. Rev. Nutr. 24, 55-78 https://doi.org/10.1146/annurev.nutr.24.012003.132258
  13. Nakamura, T., S. Matsugo, Y. Uzuka, S. Matsuo and H. Kawagishi. 2004. Fractionation and anti-tumor activity of the mycelia of liquid-cultured Phellinus linteus. Biosci. Biotech. Biochem. 68, 868-872 https://doi.org/10.1271/bbb.68.868
  14. Nanji, A. A., K. Jokelainen, G. L. Tipoe, A. Rahemtulla, P. Thomas and A. J. Dannenberg. 2003. Curcumin prevents alcohol-induced liver disease in rats by inhibiting the expression of NF-kappa B-dependent genes. Am. J. Physiol. Gastrointest. Liver Physiol. 284, G321-327 https://doi.org/10.1152/ajpgi.00230.2002
  15. Qiu, D. K., X. Ma, Y. S. Peng and X. Y. Chen. 2002. Significance of cyclooxygenase-2 expression in human primary hepatocellular carcinoma. World J. Gastroenterol. 8, 815-817 https://doi.org/10.3748/wjg.v8.i5.815
  16. Takeda, Y., A. Ichihara, H. Tanioka and H. Inove. 1964. The biochemistry of enzyme from dispersed rat liver cell. J. Biol. Chem. 239, 3590-3596
  17. Vos, T. A., A. S. H. Gouw, P. A. Klok, R. Havinga, H. V. Goor, S. Huitema, H. Roelofsen, F. Kuipers, P. L. M. Jansen and H. Moshage. 1997. Differential effects of nitric oxide synthase inhibitors on endotoxin-induced liver damage in rats. Gastroenterology 113, 1323-1333 https://doi.org/10.1053/gast.1997.v113.pm9322528

피인용 문헌

  1. Traditional uses, fermentation, phytochemistry and pharmacology of Phellinus linteus : A review vol.113, 2016, https://doi.org/10.1016/j.fitote.2016.06.009