Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Development of Soluble Glucan Production Process with Continuous Stirred Tank Fermentor

연속 발효조를 이용한 soluble glucan 생산 공정 개발

  • Moon, Chan-Jun (Department of Chemical Engineering, Chosun University) ;
  • Lee, Jung-Heon (Department of Chemical Engineering, Chosun University)
  • 문찬준 (조선대학교 공과대학 화학공학과) ;
  • 이중헌 (조선대학교 공과대학 화학공학과)
  • Published : 2006.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

Continuous fermentation process for the production of soluble glucan using mutant Agrobacterium sp. ATCC31750 has been developed in this research. When the concentration of soluble glucan was higher than 6 g/l, the viscosity of the fermented broth was too high and it needs complex separation process to separate from culture broth. Mathematical models which describe the cell growth and glucan production was developed and they kinetic parameters were estimated with experimental data. They are used for the optimization of continuous fermentation process and calculate optimal dilution rate for easy separation of glucan 4 g/l. With continuous fermentation, glucan production rate was increased 1.8 times more than that with batch fermentation.

Keywords

References

  1. Altintas, M. M., C. K. Eddy, M. Zhang, J. D. McMillan and D.S. Kompala. 2006. Kinetic modeling to optimize pentose fermentation in Zymomonas mobilis. Biotechnol. Bioeng. 94(2), 273-95 https://doi.org/10.1002/bit.20843
  2. Cheema, J. J., N. V. Sankpal, S. S. Tambe and B. D. Kulkarni. 2002. Genetic programming assisted stochastic optimization strategies for optimization of glucose to gluconic acid fermentation. Biotechnol. Prog. 18(6), 1356-65 https://doi.org/10.1021/bp015509s
  3. Chen, Q., W. E. Bentley and A. Weigand. 1995. Optimization for a recombinant E. coli fed-batch fermentation, Appl. Biochem. Biotechnol. 51, 449-454 https://doi.org/10.1007/BF02933447
  4. da Costa, A. C. and R. M. Filho. 2004. Evaluation of optimization techniques for an extractive alcoholic fermentation process. Appl. Biochem. Biotechnol. 113-116, 485-96
  5. Ganusov, V. V., A. V. Bril'kov and N. S. Pechurkin. 2000. Mathematical modeling of population dynamics of unstable plasmid-containing bacteria during continuous cultivation in a chemostat. Biofizika 45(5), 908-914
  6. Gron, S, K. V. Jochumsen, K. Biedermann and C. Emborg. 1996. Mathematical modeling of proteinase A overproduction by Saccharomyces cerevisiae. Ann. N. Y. Acad. Sci. 782, 350-362 https://doi.org/10.1111/j.1749-6632.1996.tb40574.x
  7. Miller, G. L. 1959, Use of dinitrosalicylic acid reagent for determination of reducing sugar, Anal. Chem. 31, 426-432 https://doi.org/10.1021/ac60147a030
  8. Millitzer, M, E. Wenzig and W. Peukert. 2005. Process modeling of in situ-adsorption of a bacterial lipase. Biotechnol. Bioeng. 92(6), 789-801 https://doi.org/10.1002/bit.20661
  9. Srienc, F., F. Arnold and J. E. Bailey. 1984. Characterization of intracellular accumulation of $poly-{\beta}-hydroxybutyrate$ (PHB) in individual cells of Alcaligenes eutrophus H16 by flow cytometry, Biotechnol. Bioeng. 26, 982-988 https://doi.org/10.1002/bit.260260824
  10. Yu, J and Y Si. 2001. A dynamic study and modeling of the formation of polyhydroxyalkanoates combined with treatment of high strength wastewater. Environ. Sci. Technol. 35(17), 3584-8 https://doi.org/10.1021/es001849i