Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Comparison of 99mTc-Tin colloid colloid and 99mTc-DISIDA Hepatoscintigraphy in Miniature Pigs

미니돼지에서 99mTc-Tin colloid와 99mTc-DISIDA를 사용한 간신티그라피의 비교 연구

  • Shim, Kyung-Mi (College of Veterinary Medicine Bio-therapy Human Resources Center and Biotechnology Research Institute, Chonnam National University) ;
  • Kim, Se-Eun (College of Veterinary Medicine Bio-therapy Human Resources Center and Biotechnology Research Institute, Chonnam National University) ;
  • Lee, Won-Guk (Department of Nuclear Medicine, College of Medicine, Chungbuk National University) ;
  • Koong, Sung-Soo (Department of Nuclear Medicine, College of Medicine, Chungbuk National University) ;
  • Bae, Chun-Sik (College of Veterinary Medicine Bio-therapy Human Resources Center and Biotechnology Research Institute, Chonnam National University) ;
  • Lee, Jae-Yeong (College of Veterinary Medicine, Chungbuk National University) ;
  • Choi, Seok-Hwa (College of Veterinary Medicine, Chungbuk National University) ;
  • Han, Ho-Jae (College of Veterinary Medicine Bio-therapy Human Resources Center and Biotechnology Research Institute, Chonnam National University) ;
  • Kang, Seong-Soo (College of Veterinary Medicine Bio-therapy Human Resources Center and Biotechnology Research Institute, Chonnam National University) ;
  • Park, Soo-Hyun (College of Veterinary Medicine Bio-therapy Human Resources Center and Biotechnology Research Institute, Chonnam National University)
  • 심경미 (전남대학교 수의과대학 바이오치료 산업인력 양성팀 및 생물공학연구소) ;
  • 김세은 (전남대학교 수의과대학 바이오치료 산업인력 양성팀 및 생물공학연구소) ;
  • 이원국 (충북대학교 의과대학 핵의학교실) ;
  • 궁성수 (충북대학교 의과대학 핵의학교실) ;
  • 배춘식 (전남대학교 수의과대학 바이오치료 산업인력 양성팀 및 생물공학연구소) ;
  • 이재영 (충북대학교 수의과대학) ;
  • 최석화 (충북대학교 수의과대학) ;
  • 한호재 (전남대학교 수의과대학 바이오치료 산업인력 양성팀 및 생물공학연구소) ;
  • 강성수 (전남대학교 수의과대학 바이오치료 산업인력 양성팀 및 생물공학연구소) ;
  • 박수현 (전남대학교 수의과대학 바이오치료 산업인력 양성팀 및 생물공학연구소)
  • Published : 2006.10.01
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Abstract

Non-invasive evaluation of liver function in animal models remains a challenge. Hepatoscintigraphy provides information about changes in liver size and shape, and enables to understand general liver function. Futhermore it is readily used to diagnosis complications of liver transplantation like hepatitis, rejections and biliary complications. In this study, we investigated the usefulness of evaluating the liver function in miniature pigs with $^{99m}Tc-Tin$ colloid and $^{99m}Tc-DISIDA$ which are the most commonly used radiopharmaceuticals in human medicine. In result, $^{99m}Tc-Tin$ colloid was uptaked in lung, liver, gastric wall and kidney in miniature pigs. And $^{99m}Tc-DISIDA$ showed continuous uptake images of heart, lung, liver, gallbladder and duodenum, and it was similar to human's. Therefore we could conclude $^{99m}Tc-Tin$ colloid would not be suitable for evaluating hepatic function because of it's nonspecific affinity, however $^{99m}Tc-DISIDA$ scintigraphy would be an effective method for detecting hepatobiliary function in miniature pigs.

동물 모델에서의 간기능의 비침습적 평가는 하나의 과제로 남아있다. 간신티그라피는 간의 크기 및 모양의 변화에 대한 정보를 제공할 뿐 아니라 간의 전체적인 기능을 파악할 수 있게 해 주며 간이식의 주요 합병증인 간염, 거부반응 및 담도계 합병증의 진단에도 편리하게 사용될 수 있다. 이에 본 연구에서는 미니돼지의 간 기능을 평가하기 위해 사람에서 주로 사용되는 방사성의약품인 $^{99m}Tc-Tin$ colloid와 $^{99m}Tc-DISIDA$를 이용하여 간 및 간담도 신티그라피를 시행하였다. 그 결과, 사람의 간신티그라피와 달리 미니돼지에서는 $^{99m}Tc-Tin$ colloid가 폐, 간, 위벽 및 신장에 섭취되고 폐와 간의 영상이 겹쳐져 간의 관심영역을 설정할 수 없었다. 그러나 $^{99m}Tc-DISIDA$를 사용한 간담도 신티그라피에서는 심장, 폐, 간, 담낭 및 십이지장으로의 연속적 방사능섭취를 관찰할 수 있었다. 따라서 미니돼지에서의 $^{99m}Tc-Tin$ colloid의 사용은 비특이적 결합력으로 인해 간 기능 평가를 위한 영상에는 적합하지 않으나 $^{99m}Tc-DISIDA$는 간담도 기능을 파악하는데 있어 좋은 수단이 될 수 있을 것으로 생각된다.

Keywords

References

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