Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Efficient Regeneration of Plants Independent of Exogeneous Growth Regulators Using Tissues in Pepper (Capsicum annuum L.)

고추의 조직을 이용한 생장조절제 처리가 식물체 재분화 효율에 미치는 영향

  • Lee, Moon-Jung (Gyeungbuk Agricultural Technology Administration) ;
  • Kwon, Tae-Roung (Gyeungbuk Agricultural Technology Administration) ;
  • Shin, Dong-Hyun (Department of Agronomy, College of Agriculture and Life Sciences, Kyunpook National University) ;
  • Harn, Jung-Sul (National Horticultural Research Institute, Rural Development Administration) ;
  • Kim, Kyung-Min (Department of Environment Horticulture, Sangju National University) ;
  • Kim, Chang-Kil (Department of Environment Horticulture, Sangju National University) ;
  • Oh, Jung-Youl (Department of Environment Horticulture, Sangju National University)
  • Published : 2006.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

The study was carried out to develop efficiency of transformantion in pepper plants. We used cotyledons of two native varieties, 'Subicho' and 'Kumtap' to establish some conditions of plant regeneration. Differentiation rate of shoot was higher in 2 to 4 mg/l zeatin and 0.05 mg/l NAA than in other treatments. Meanwhile, differential rate of roots was the highest in 0.5 mg/l NAA.

재래종 고추인 '수비초'와 ‘금탑’의 자엽을 이용한 식물체재분화 조건을 구명하기 위해 시험한 결과, shoot 분화율은 $2{\sim}4\;mg/l$ zeatin 에서 0.05 mg/l NAA 혼용에서 분화율이 높았고, 뿌리 분화율은 0.5 mg/l NAA 에서 높았다.

Keywords

References

  1. Agrawal, S., N. Chandra and S. L. Kothari. 1989. Plant generation in tissue cultures of pepper(Capsicum annuum L. ev. Mathania). Plant Cell Tiss. Org. Cult. 16, 47-55 https://doi.org/10.1007/BF00044071
  2. Arroyo, R. and M. Angeles Revilla. 1991. In vitro plant regeneration from cotyledon and hypocotyl segments in two bell pepper cultivars. Plant Cell Reports 10, 414-416
  3. Binzel, M. L., N. Sankhla, S. Joshi and D. Sannkhla. 1996. Induction of direct somatic embryogenesis and plant regeneration in pepper(Capsicum annuum L.). Plant Cell Reports 15, 536-540 https://doi.org/10.1007/BF00232989
  4. Christopher, T. and M. V. Rajam. 1996. Effect of genotype, explant and medium on in vitro regeneration of red pepper. Plant Cell Tiss. Org. Cult. 6, 45-250
  5. Dabauza, M. and L. Pena. 2001. High efficiency organogenesis in sweet pepper (Capsicum annuum L.) tissues from different seedling explants. Plant Growth Regulation 33, 221-229 https://doi.org/10.1023/A:1017585407870
  6. Diaz, I., R. Moreno and J. B. Power. 1988. Plant regeneration from protoplasts of Capsicum annuum. Plant Cell Reports 7, 210-212 https://doi.org/10.1007/BF00269326
  7. Dix, P. J. and H. E. Street. 1976. Selection of plant cell lines with enhanced chilling resistance. Ann. Bot. 40, 903-910 https://doi.org/10.1093/oxfordjournals.aob.a085222
  8. Ezura, H., N. Satoshi and M. Kasumi. 1993. Efficient regeneration of plants independent of exogenous growth regulators in bell pepper(Capsicum annuum L.). Plant Cell Reports 12, 676-680
  9. Franck-Duchenne, M., Y. Wang, S. F. Tahar and R. N. Beachy. 1998. In vitro stem elongation of sweet pepper in media containing 24-epi-brassinolide. Plant Cell Tiss. Org. Cult. 53, 79-84 https://doi.org/10.1023/A:1006071803855
  10. Gunay, A. L. and P. S. Rao. 1978. In vitro plant regeneration from hypocotyl and cotyledon explants of red pepper(Capsicum). Plant Sci. Lett. 11, 365-372 https://doi.org/10.1016/0304-4211(78)90024-X
  11. Husain, S., A. Jain and S. L. Kothari. 1999. Phenylacetic acid improves bud elongation and in vitro plant regeneration efficiency in C. annuum L. Plant Cell Reports 19, 64-68 https://doi.org/10.1007/s002990050711
  12. Kintzios, S., J. B. Drossopoulos, E. Shortsianitis and D. Peppes. 2000. Induction of somatic embryogenesis from young, fully expanded leaves of chilli pepper (Capsicum annuum L.): effect of leaf position, illumination and explant pretreatment with high cytokinin concentrations. Scientia Horticulturae 85, 137-144 https://doi.org/10.1016/S0304-4238(99)00135-1
  13. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant 5, 473-479
  14. Ochoa-Alejo, N. and L. Ireta-Moreno. 1990. Cultivar differences in shoot-forming capacity of hypocotyl tissues of chilli pepper (C. annuum) cultured in vitro. Scientia Horticulturae 42, 21-28 https://doi.org/10.1016/0304-4238(90)90144-4
  15. Phillips, G. C. and J. F. Hubstenberger. 1985. Organogenesis in pepper tissue cultures. Plant Cell Tiss. Org. Cult. 4, 261-269 https://doi.org/10.1007/BF00040200
  16. Pozueta-Romero, J., G. Houlne, L. Canas, R. Schantz and J. Chamarro. 2001. Enhanced regeneration of tomato and pepper seedling explants for Agrobacterium-mediated transformation. Plant Cell Tiss. Org. Cult. 67, 173-180 https://doi.org/10.1023/A:1011997926381
  17. Ramirez-Malagon, R. and N. Ochoa-Alejo. 1996. An improved and reliable chili pepper(C. annuum L.) plant regeneration method. Plant Cell Reports 16, 226-231 https://doi.org/10.1007/BF01890873
  18. Scripichitt, P., E. Nawata and S. Shigenaga. 1987. In vitro shoot-forming capacity of cotyledon explants in red pepper(C. annuum L. cv. Yatsufusa). Japan J. Breed. 37, 133-142 https://doi.org/10.1270/jsbbs1951.37.133
  19. Wang, Y., M. Yang, N. Pan and Z. Chen. 1991. Plant regeneration and transformation of sweet pepper(Capsicum frutescens). Acta. Bot. Sin. 33, 780-786