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The Ecological Character and Sequence Similarity Analysis of 18S rDNA from the Two Species of Grateloupia (Grateloupia, Rhodophyta) in Korea

두 종의 한국산 홍조 지누아리류(Grateloupia filicina, Grateloupia divaricata)의 생태특성과 18S rDNA 염기서열 상동성 분석

  • Kim, Young-Dae (Aquaculture Division, East Sea Regional Fisheries Research Institute) ;
  • Jin, Long-Guo (Department of Biotechnology, Pukyong National University) ;
  • Song, Hong-In (Aquaculture Division, East Sea Regional Fisheries Research Institute) ;
  • Hong, Jung-Pyo (Aquaculture Division, East Sea Regional Fisheries Research Institute) ;
  • Lee, Chu (Aquaculture Division, East Sea Regional Fisheries Research Institute) ;
  • Jeon, Chang-Young (Aquaculture Division, East Sea Regional Fisheries Research Institute) ;
  • Kim, Su-Kyoung (Aquaculture Division, East Sea Regional Fisheries Research Institute) ;
  • Kim, Dong-Sam (Aquaculture Division, East Sea Regional Fisheries Research Institute) ;
  • Han, Hyoung-Kyun (Aquaculture Division, East Sea Regional Fisheries Research Institute) ;
  • Hong, Yong-Ki (Department of Biotechnology, Pukyong National University)
  • 김영대 (국립수산과학원동해수산연구소) ;
  • ;
  • 송홍인 (국립수산과학원동해수산연구소) ;
  • 홍정표 (국립수산과학원동해수산연구소) ;
  • 이주 (국립수산과학원동해수산연구소) ;
  • 전창영 (국립수산과학원동해수산연구소) ;
  • 김수경 (국립수산과학원동해수산연구소) ;
  • 김동삼 (국립수산과학원동해수산연구소) ;
  • 한형균 (국립수산과학원동해수산연구소) ;
  • 홍용기 (부경대학교 생물공학과)
  • Published : 2006.08.30

Abstract

The species of Grateloupia filicina, Grateloupia divaricata in East Sea were investigated taxonmically in order to clarify taxonomic position. The ecological character, external morphology, anatomy of vegetative structure. Blade length are $15{\sim}40\;cm$, erect from discoidal holdfast of $3{\sim}10\;mm$ in diameter. Stipe $1{\sim}2.5\;cm$ long, narrowly cylindrical below, compressed above Grateloupia filicina. Main axis are long and compressed, $3{\sim}7\;mm$ broad in broadest part. Colors are scarlet to light red. Blade length are $10{\sim}25\;cm$, erect from discoidal holdfast of $3{\sim}8\;cm$ in diameter Grateloupia divaricata. Stipe are single and simple $2{\sim}5\;mm$ broad. Thallus composed of cortex and medulla in section ; cortex composed of $9{\sim}10$ layers of anticlinally arranged cortical cell, divided into outer, middle and inner parts. Partial fragments of nuclear 18S rDNAs from the two species of Grateloupia (Rhodophyta) were amplified using the PCR reaction and sequenced to compare their similarity. The partial sequences showed 98.9% similarity each other. Grateloupia filicina has 371 bp sizes and Grateloupia divaricata has 372 bp size. The G+C contents of Grateloupia filicina is 54.3% and Grateloupia divaricata is 53.64%.

Keywords

References

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