생명과학회지 (Journal of Life Science)

  • 제16권5호
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  • Pages.716-722
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  • 2006
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
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대장균에서의 human SOD1과 mutant SOD1 (G93A) 단백질의 발현과 HtrA2의 기질 여부 확인에 관한 연구

Expression of Human SOD1 and Mutant SOD1 (G93A) in E. coli and Identification of SOD1 as a Substrate of HtrA2 Serine Protease

  • 김구영 (가톨릭대학교 생명의과학과,분자유전학연구소) ;
  • 김상수 (가톨릭대학교 생명의과학과,분자유전학연구소) ;
  • 박효진 (가톨릭대학교 분자유전학연구소,고려대학교 생명과학대학 생명과학부) ;
  • 임향숙 (가톨릭대학교 생명의과학과,분자유전학연구소)
  • Kim, Goo-Young (Department of Biomedical Sciences,Research Institute of Molecular Genetics, College of Medicine, the Catholic University of Korea) ;
  • Kim, Sang-Soo (Department of Biomedical Sciences,Research Institute of Molecular Genetics, College of Medicine, the Catholic University of Korea) ;
  • Park, Hyo-Jin (Research Institute of Molecular Genetics, College of Medicine, the Catholic University of Korea,School of Life Science and Biotechnology, Korea University) ;
  • Rhim, Hyang-Shuk (Department of Biomedical Sciences,Research Institute of Molecular Genetics, College of Medicine, the Catholic University of Korea)
  • 발행 : 2006.08.30
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초록

Superoxide dismutase (SOD) is physiologically important in regulating cellular homeostasis and apoptotic cell death, and its mutations are the cause of familial amyotrophic lateral sclerosis (FALS). Mitochondrial serine protease HtrA2 has a pro-apoptotic function and has known to be associated with neurodegenerative disorders. To investigate the relationship between genes associated with apoptotic cell death, such as HtrA2 and SOD1, we utilized the pGEX expression system to develop a simple and rapid method for purifying wild-type and ALS-associated mutant SOD1 proteins in a suitable form for biochemical studies. We purified SOD1 and SOD1 (G93A) proteins to approximately 90% purity with relatively high yields (3 mg per liter of culture). Consistent with the result in mammalian cells, SOD1 (G93A) was more insoluble than wild-type SOD1 in E. coli, indicating that research on the aggregate formation of SOD1 may be possible using this pGEX expression system in E. coli. We investigated the HtrA2 serine protease activity on SOD1 to assess the relationship between two proteins. Not only wild-type SOD1 but also ALS-associated mutant SOD1 (G93A) were cleaved by HtrA2, resulting in the production of the 19 kDa and 21 kDa fragments that were specific for anti-SOD1 antibody. Using protein gel electrophoresis and immunoblot assay, we compared the relative molecular masses of thrombin-cleaved GST-SOD1 and HtrA2-cleaved SOD1 fragments and can predict that the HtrA2-cleavage sites within SOD1 are the peptide bonds between leucine 9-lysine 10 (L9-K10) and glutamine 23-lysine 24 (Q23-K24). Our study indicates that SOD1 is one of the substrate for HtrA2, suggesting that both HtrA2 and SOD1 may be important for modulating the HtrA2-SOD1-mediated apopotic cell death that is associated with the pathogenesis of neurodegenerative disorder.

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피인용 문헌

  1. Purification of Human HtrA1 Expressed in E. coli and Characterization of Its Serine Protease Activity vol.16, pp.7, 2006, https://doi.org/10.5352/JLS.2006.16.7.1133