Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Characteristics of Insoluble Phosphates Solubilizing by Klebsiella sp. DA71-1/pLYJ

Klebsiella sp. DA71-1/pLYJ의 난용성 인산염 가용화 특성

  • Ryu, Ah-Reum (Department of Biotechnology, Faculty of Natural Resource and Life Science Dong-A University) ;
  • Lee, Jin-Woo (Department of Biotechnology, Faculty of Natural Resource and Life Science Dong-A University) ;
  • Lee, Yong-Seok (Department of Biotechnology, Faculty of Natural Resource and Life Science Dong-A University) ;
  • Lee, Sang-Cheol (Department of Biotechnology, Faculty of Natural Resource and Life Science Dong-A University) ;
  • Chung, Soo-Yeol (Department of Food Science, Dongju College) ;
  • Choi, Yong-Lark (Department of Biotechnology, Faculty of Natural Resource and Life Science Dong-A University)
  • 류아름 (동아대학교 응용생명공학부) ;
  • 이진우 (동아대학교 응용생명공학부) ;
  • 이용석 (동아대학교 응용생명공학부) ;
  • 이상철 (동아대학교 응용생명공학부) ;
  • 정수열 (동주대학 식품과학계열) ;
  • 최용락 (동아대학교 응용생명공학부)
  • Published : 2006.07.31
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Abstract

To develop high efficiency biofertilizer solubilizing insoluble phosphates, lactate dehydrogenase (ldh) gene was isolated from Staphylococcus sp. LJ2. Genetic constructions were carried out using the pGEM-T-easy vector and pHSG398. Recombinant DNA plasmids containing the ldh gene were transferred to Klebsiella sp. DA71-1 by electroporation. The selected transformant was named as a DA71-1/pLYJ. The insoluble phosphates solubilization activity of DA71-1/pLYJ was higher than that of DA71-1 at various culture conditions. Glucose was the best carbon source for insoluble phosphates solubilization among the used carbon sources. Maximal insoluble phosphates solubilizing was found in sucrose minimal (SM) medium containing 3% glucose. The solubilizing activity of DA71-1/pLYJ against three types of insoluble phosphates, such as tri-calcium phosphate, hydroxyapatite, aluminium phosphate, were quantitatively determined. The optimal temperature and initial pH to solubilize insoluble phosphates in the SM medium was $37^{\circ}C$ and pH 5.0, respectively.

친환경형 미생물제제의 난용성 인산염 가용화능의 향상을 위하여 Staphylococcus sp. LJ2로부터 분리된 ldh gene을 Klebsiella sp. DA71-1에 도입하였고, 이를 DA71-1/pLYJ라고 명명하였다. 배지에 glucose를 3% 첨가했을 때 다른 탄소원을 첨가했을 때보다 DA71-1/pLYJ 균주의 난용성 인산염 가용화능이 월등히 우수하였다. 각기 다른 난용성 인산염 tri-calcium phosphate, hydroxyapatite, 그리고 aluminium phosphate에 대하여 그 가용화능이 DA71-1 균주보다 적게는 1.2배, 많게는 2.3배 이상 향상된 결과를 보였다. 그리고 DA71-1/pLYJ 균주는 DA71-1 균주에 비해 배양 온도에 비교적 관계없이 높은 인산가용화능을 나타낸 것이 특징적이었고, 배양초기 pH가 5.0일 때 그 능력이 가장 뛰어났다. 이러한 모든 결과들을 종합해볼 때 DA71-1/pLYJ 균주는 여러면에서 DA71-1 균주보다 난용성 인산염 가용화능이 월등히 뛰어나며 따라서 효율적이고 친환경적인 미생물제제의 개발에 큰 역할을 할 수 있는 발판을 마련했다 할 수 있다.

Keywords

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