생명과학회지 (Journal of Life Science)

  • 제16권4호
  • /
  • Pages.683-690
  • /
  • 2006
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

함초(Salicornia herbacea L.)의 채취 시기별 이화학적 특성 탐색

Detection of Chemical Characteristics in Hamcho (Salicornia herbacea L.) according to Harvest Periods

  • Cha, Jae-Young (Alcoholic Beverage Research Institute, Daesun Distilling Co., Ltd.) ;
  • Jeong, Jae-Jun (Alcoholic Beverage Research Institute, Daesun Distilling Co., Ltd.) ;
  • Kim, Yong-Taek (Alcoholic Beverage Research Institute, Daesun Distilling Co., Ltd.) ;
  • Seo, Won-Seok (Alcoholic Beverage Research Institute, Daesun Distilling Co., Ltd.) ;
  • Yang, Hyun-Ju (Alcoholic Beverage Research Institute, Daesun Distilling Co., Ltd.) ;
  • Kim, Jin-Sook (Alcoholic Beverage Research Institute, Daesun Distilling Co., Ltd.) ;
  • Lee, Yong-Soo (Alcoholic Beverage Research Institute, Daesun Distilling Co., Ltd.)
  • 발행 : 2006.07.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

함초의 생리활성 물질을 활용하여 기능성 식품을 개발하기 위한 기초 자료로 이용하기 위하여 함초의 채취 시기별 함초의 수용성 추출물의 폴리페놀 화합물 함량은 건물 중량 100 g당 6월 0.682%, 8월 1.005% 및 10월 0.754%였으며, 에탄올 추출물에서는 6월 1.015%, 8월 1.158% 및 10월에 0.953%로 8월 에탄올 추출물에서 가장 높았다. 함초 수용성 추출물의 betaine 성분을 HPLC를 이용하여 측정한 결과 6월, 8월, 10월에서 각각 0.248%, 0.269% 및 0.204%로 나타났다. 함초 성숙 단계별로 채취한 시료의 주요 구성 아미노산 함량(mg%)이 6월, 8월, 10월에서 각각 glutamic acid (582: 519: 664), proline (552: 471: 322), phenylalanine (480: 431: 424), aspartic acid (322: 297: 330), 및 arginine (282: 321: 483)이었으며, 주요 유리 아미노산 함량 (mg%)은 6월과 8월에 각각 proline (9.7: 3.4), asparagine (6.7: 1.4), hydroxyproline (6.4: 2.8), valine (3.9: 2.5) 및 arginine (1.7: 3.0) 이었다. 그러나 10월에 채취된 함초의 유리 아미노산은 유일하게 amonium chloride 0.27 mg%와 1-methyl-L-histidine 0.18 mg%만 함유되어 있었다. 채취 시기별 함초의 미네랄 성분 함량 (mg%)은 전체적으로 Na이 가장 많았고 그 다음으로 K, Mg, Ca, P 순이었다. 채취 시기별로 미네랄 함량이 각각 Na (5695: 7536: 5529), K (1640: 963: 931), Mg (359: 428: 348), Ca (221: 234: 251) 및 P (207: 189: 259) 순으로 함유되어 있었고, 6월에 K, 8월에 Na와 Mg, 10월에 Ca과 P가 각각 높게 함유되어 있었다. 전자공여능(EDA) 측정에서는 0.05% BHT> 8월> 6월> 10월 순으로 항산화 활성을 나타내었다. 이상의 결과로 함초의 이화학적 성분은 채취 시기별로 다르게 나타났으며, 폴리페놀 화합물 및 베타인의 생리활성 성분은 8월에 채취한 함초에서 가장 높았다.

The chemical compositions of amino acids, minerals, betaine, and polyphenolic compounds from Salicornia herbacea (Hamcho) according to harvest periods were analyzed. Changes of chemical characteristics in water and ethanol extracts from Hamcho were evaluated for titratable acidity, pH, soluble solid, and Hunter's color values. The antioxidative activity of water extract from Hamcho was also determined by DPPH $({\alpha},{\alpha}'-diphenyl-{\beta}-picrylhydrazyl)$ scavenging radical activity. Total polyphenolic compounds of Hamcho were shown the highest in August harvested by 201.6 ppm. The betaine of Hamcho water-extract was identified by high performance liquid chromatography (HPLC), which content was 0.248%, 0.269% and 0.204% in June, August, and October, respectively. Major compositional amino acids (mg%) were glutamic acid (582: 519: 664), proline (552: 471: 322), phenylalanine (480: 431: 424), aspartic acid (322: 297: 330), and arginine (282: 321: 483) in June, August, and October, respectively, and major free amino acids (mg%) were proline (9.7: 3.4), asparagine (6.7: 1.4), hydroxyproline (6.4: 2.8), valine (3.9: 2.5), arginine (1.7: 3.0) in June and August, respectively. Mineral contents (mg%) were Na (5,695: 7,536: 5,529), K (1,640: 963: 931), Mg (359: 428: 348), Ca (221: 234: 251), and P (207: 189: 259) in June, August, and October, respectively. Especially, K was high in June, Na and Mg were high in August, and Ca and P were high in October, respectively. DPPH scavenging radical activity was shown in the following order; 0.05% butylated hydroxytoluene (BHT)> August> June> October harvested Hamcho. The chemical components of polyphenolic compounds, betaine, amino acids and minerals were changed by harvest periods according to the growing season, and the highest concentrations of polyphenolic compounds and betaine of Hamcho were shown in August harvested.

키워드

참고문헌

  1. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1204
  2. Cha, J. Y. and Y. S. Cho. 1997. Effects of hesperidin, naringin and their aglycones on the in vitro assay phosphatidate phosphohydrolase, and on the proliferation in cultured human hepatocytes HepG2 cells. Agri. Chem. Biotech. 40, 577-582
  3. Cha, J. Y. and Y. S. Cho. 1999. Effect of potato polyphenolics on lipid peroxidation in rats. J. Korean Soc. Food Sci. Nutr. 28, 1131-1136
  4. Cha, J. Y., B. S. Jeon, J. W. Park, B. K. Kim, C. Y. Jeong, J. S. Ryu, C. K. Choi and Y. S. Cho. 2004. Hypocholesterolemic effect of yogurt supplemented Saliconia herbacea extract in cholesterol-fed rats. Korean J. Life Sci. 14, 747-751 https://doi.org/10.5352/JLS.2004.14.5.747
  5. Cho, K. J., Y. S. Lee and B. H. Ryu. 1990. Antitumor effect and immunology activity seaweeds toward sarcoma-180. J. Korean Fish Soc. 23, 345-352
  6. Choi, O. J. and K. H. Choi. 2003. The physiological properties of Korean wild teas (green tea, semi-fermented tea, and black tea) according to degree of fermentation. J. Korean Soc. Food Sci. Nutr. 32, 356-362 https://doi.org/10.3746/jkfn.2003.32.3.356
  7. Han, S. K. and M. S. Kim. 2003. Antioxidative effect of Salicornia herbacea L. grown in closed sea beach. J. Korean Soc. Food Sci. Nutr. 32, 207-210 https://doi.org/10.3746/jkfn.2003.32.2.207
  8. Han, S. K., M. S. Kim and B. S. Pyo. 2003. Antioxidative effect of glasswort (Salicornia herbacea L.) on the lipid oxidation of pork. Korean J. Food Sci. Ani. Resour. 23, 46-49
  9. Ihm, B. S. and J. S. Lee. 1986. The strategies of Salicornia herbacea and Suaeda japonica for coping with environmental fluctuation of salt marsh. Korean J. Environ. Biol. 4, 15-25
  10. Im, S. A., G. W. Kim and C. K. Lee. 2003. Immunomodulatory activity of Salicornia herbacea L. components, Nat. Prod. Sci. 9, 273-277
  11. Jo, Y. C., J. H. Ahn, S. M. Chon, K. S. Lee, T. J. Bea and D. S. Kang. 2002. Studies on pharmacological effects of glasswort (Salicornia herbacea L.). Korean J. Medicinal Corp Sci. 10, 93-99
  12. Kim, E. S., K. J. Im, H. Park and S. K. Chun. 1978. Studies on amino acid composition of Korean foods (I). Korean J. Food Sci. Technol. 10, 371-378
  13. Kim, C. S. and T. G. Song. 1983. Ecological studies on the halophyte communities at Western and Southern coasts in Korea, Korean J. Ecology 6, 167-176
  14. Kim, S. K., J. Y. Cha, S. J. Jeong, C. H. Chung, Y. L. Choi and Y. S. Cho. 2000. Properties of the chemical composition of safflower (Carthamus tinctorius L.) sprout. J. Life Sci. 10, 68-73
  15. Kim, D. S. and C. H. Kim. 2001. Effect of sea tangle, Laminaria japonicus, extract on activities of glucokinase and hexokinase in alloxan-induced diabetic mellitus mice. Korean J. Life Sci. 11, 467-482
  16. Kim, Y. S., M. R. Huh and J. C. Park. 2001. Effects of culture media and seawater on growth and mineral concentrations in glasswort (Salicornia herbacea). Korean J. Hort. Sci. Technol. 19, 342-347
  17. Lee, C. H., I. H. Kim, Y. E. Kim, S. W. Oh and H. J. Lee. 2004. Determination of betaine from Saliconia herbacea L. J. Korean Soc. Food Sci. Nutr. 33, 1584-1587 https://doi.org/10.3746/jkfn.2004.33.9.1584
  18. Lee, J. H. and S. R. Lee. 1994. Analysis of phenolic substances content on Korea plant foods. Korean J. Food Sci. Technol. 26, 310-316
  19. Lee, H. S., M. S. Choi, Y. K. Lee, S. H. Park and Y. J. Kim. 1996. A study on the development of high-fiber supplements for the diabetic patients (2)-Effect of seaweed supplementation on the lipid and glucose metabolism in streptozotocin-induced diabetic rats. Korean J. Nutr. 29, 296-306
  20. Lee, K. S., J. S. Seo and Y. S. Choi. 1998. Effect of sea tangle and hypoglycemic agent on lipid metabolism in diabetic rats. J. Korean Soc. Food Sci. Nutr. 27, 960-967
  21. Lee, J. J., C. S. Kim, S. H. Kim, C. S. Huh and Y. H. Baek. 1999. Changes of polyphenol contents in unripe apples according to heat treatments. Korean J. Food Sci. Technol. 31, 147-152
  22. Lee, E. J. and M. K. Sung. 2001. Effect of fiber-rich sea mustard feeding on AOM-induced colon aberrant crypt formation and colonic cell proliferation in Sprague Dawley rats. J. Korean Soc. Food Sci. Nutr. 30, 535-539
  23. Lee, J. T., Y. S. Jeong and B. J. An. 2002. Physiological activity of Salicornia herbacea and Its application for cosmetic materials. Kor. J. Hearology 17, 51-60
  24. Lee, Y. S., H. S. Lee, K. H. Shin, B. K. Kim and S. Lee. 2004. Constituents of the halophyte Salicornia herbacea. Arch. Pharm. Res. 27, 1034-1036 https://doi.org/10.1007/BF02975427
  25. Park, E. I. and T. A. Garrow. 1999. Interaction between dietary methionine and methyl donor intake on rat liver betaine- homocysteine methyltransferase gene expression and organization of the human gene. J. Biol. Chem. 274, 7816-7824 https://doi.org/10.1074/jbc.274.12.7816
  26. Park, J. H., K. S. Kim and H. K. Choi. 1997. Studies on the free amino acid, organic acid and fatty acid content of Korean tea plants. J. Korean Tea Soc. 3, 73-87
  27. Park, S. H. and K. S. Kim. 2004. Isolation and identification of antioxidant flavonoids from Salicornia herbacea. J. Korean Soc. Appl. Biol. Chem. 47, 120-123
  28. Swain, T., W. E. Hillis and M. Oritega. 1959. Phenolic constituents of Ptunus domestica. I. Quantitative analysis of phenolic constituents. J. Sci. Food Agric. 10, 83-88

피인용 문헌

  1. Changes in Physicochemical Properties of Spergularia marina Griseb by Blanching vol.19, pp.6, 2012, https://doi.org/10.11002/kjfp.2012.19.6.866
  2. Antioxidant Activities of Extracts from Fermented Mulberry (Cudrania tricuspidata) Fruit. and Inhibitory Actions on Elastase and Tyrosinase vol.18, pp.2, 2011, https://doi.org/10.11002/kjfp.2011.18.2.236
  3. Characteristic of Glasswort (Salicornia Herbacea L.) Mixture Fermentation Utilizing Aspergillus oryzae vol.39, pp.9, 2010, https://doi.org/10.3746/jkfn.2010.39.9.1384
  4. Biological Activities of Ethanol Extracts from Monascus-Fermented Chinese Yam vol.21, pp.8, 2011, https://doi.org/10.5352/JLS.2011.21.8.1142
  5. Evaluation and comparison of functional properties of freshwater-cultivated glasswort (Salicornia herbacea L.) with naturally-grown glasswort vol.24, pp.6, 2015, https://doi.org/10.1007/s10068-015-0299-1
  6. Antioxidant and Pro-oxidant Activities of Hamcho (Salicornia herbacea L.) vol.28, pp.1, 2015, https://doi.org/10.9799/ksfan.2015.28.1.040
  7. Antioxidant activities, production of reactive oxygen species, and cytotoxic properties of fractions from aerial parts of glasswort (Salicornia herbacea L.) vol.48, pp.6, 2016, https://doi.org/10.9721/KJFST.2016.48.6.574
  8. Antioxidant activities and physiological properties ofEuphorbia humifusa extracts prepared using different solvents vol.23, pp.2, 2016, https://doi.org/10.11002/kjfp.2016.23.2.252
  9. Antioxidant and Angiotensin Converting Enzyme I Inhibitory Activities of Extracts from Mulberry (Cudrania tricuspidata) Fruit subjected to Different Drying Methods vol.41, pp.10, 2012, https://doi.org/10.3746/jkfn.2012.41.10.1388
  10. Comparison of Antioxidant and Physiological Properties of Jerusalem Artichoke Leaves with Different Extraction Processes vol.42, pp.1, 2013, https://doi.org/10.3746/jkfn.2013.42.1.068
  11. Comparison of the antioxidant effects and physiological activities of grape fruit stem extracts derived with different drying methods vol.25, pp.3, 2018, https://doi.org/10.11002/kjfp.2018.25.3.382
  12. Changes in the physicochemical characteristics of low-salt Doenjang by addition of halophytes vol.25, pp.7, 2018, https://doi.org/10.11002/kjfp.2018.25.7.819