Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Molecular Typing of Salmonella enterica serovar Typhi Strains Isolated in Busan by Pulsed-Field Gel Electrophoresis

부산지역에서 분리된 Salmonella enterica serovar Typhi균에 대한 PFGE를 이용한 Molecular typing

  • Min, Sang-Kee (Division of Microbiology, Busan Institute Of Health and Environment) ;
  • Lee, Ju-Hyun (Division of Microbiology, Busan Institute Of Health and Environment) ;
  • Park, Eun-Hee (Division of Microbiology, Busan Institute Of Health and Environment) ;
  • Kim, Jung-Ah (Division of Microbiology, Busan Institute Of Health and Environment) ;
  • Kim, Kyu-Won (College of Pharmacy, Seoul National University)
  • 민상기 (부산광역시보건환경연구원) ;
  • 이주현 (부산광역시보건환경연구원) ;
  • 박은희 (부산광역시보건환경연구원) ;
  • 김정아 (부산광역시보건환경연구원) ;
  • 김규원 (서울대학교 약학대학)
  • Published : 2006.07.31
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Abstract

We obtained 424 Salmonella enterica serovar Typhi isolates from sporadic cases of infection in Busan during 1996 to 2005. We investigated the trend of antimicrobial resistance and molecular typing by pulsed-field gel electrophoresis (PFGE). Of the total 424 isolates, 6 strains (1.4%) were multidrug-resistant (MDR) S. enterica serovar Typhi isolates, 2 strains (0.5%) were resistant to only nalidixic acid, and the remaining 416 strains (98.1%) were fully susceptible to the 18 antimicrobial agent. PFGE of XbaI-digested chromosomal DNA was performed on 50 sporadic S. enterica serovar Typhi isolates with the objective of investigating the extent of genetic diversity of these isolates in our region. We could find that these isolates were much more heterogeneous and at least 32 different PFGE patterns were generated according by dice coefficient, between 0.69 and 1.0. Restriction fragment patterns consisted of 13 to 18 fragments ranged in size from 20 to 630 kb. The results confirmed that PFGE would be an useful tool for investigating surveillance of sporadic or outbreak case and assessing clonality for S. enterica serovar Typhi in Busan area. Our finding will be valuable in developing rational strategies to control this pathogen and setting the basis of an effective PulseNet system in Korea.

1996년부터 2005년까지 부산지역에서 분리된 Salmonella enterica serovar Typhi 균주에 대한 항균제 내성 변화양상 및 Pulsed-field gel electrophoresis(PFGE)를 이용한 분리주의 분자역학적 형별을 분석하였다. 전체 424주에 대한 항균제 감수성 시험결과 multidrug-resistant (MDR) 6주(1.4%)와 nalidixic acid에만 내성을 보이는 2주를 제외한 나머지 416주(98.1%)가 시험 항균제 18종 모두에 감수성을 보였다. 부산지역 분리 장티푸스균의 유전적 이질성을 확인하고자 실시한 산발 분리 50주의 PFGE/XbaI 시험결과, 최소 32종의 다양한 패턴이 나타났다. 각 패턴별로 제한효소 절편 수는 13개에서 18개까지였고, 절편크기는 약 20 kb에서 630 kb 범위였다. 본 시험결과 부산지역의 장티푸스의 산발 또는 집단 발생시 PFGE는 유용한 역학적 지표로 사용가능함을 알 수 있었으며 또한 전국적 PulseNet 구축의 기초 자료로서 활용도가 높을 것으로 사료된다.

Keywords

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