Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Characterization of an Apple Polygalacturonase-inhibiting Protein (PGIP) from Apple Fruits

사과 과실로부터 분리한 polygalacturonase-inhibiting protein(PGIP)의 생화학적 특성

  • Lee, Dong-Hoon (Citrus Research Division, National Institute of Subtropical Agriculture, RDA.) ;
  • Kang, Sang-Gu (School of Biotechnology, Institute of Biotechnology, Yeungnam Univ.) ;
  • Kang, In-Kyu (Dept. of Enviromental Horticulture, Sangju National Univ.) ;
  • Lee, Yoon-Kyeong (Dept. of Oriental Medicine Resources, Asia Univ.) ;
  • Choi, Cheol (Division of Plant Biosciences, Kyungpook National Univ.) ;
  • Byun, Jae-Kyun (School of Natural Resources, Yeungnam Univ.)
  • 이동훈 (농촌진흥청 난지농업연구소 감귤과) ;
  • 강상구 (영남대학교 생명공학부) ;
  • 강인규 (상주대학교 환경원예학과) ;
  • 이윤경 (아시아대학교 한약자원학과) ;
  • 최철 (경북대학교 식물생명과학부) ;
  • 변재균 (영남대학교 자연자원학부)
  • Published : 2006.07.31
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Abstract

An apple polygalacturonase-inhibiting protein (PGIP), that specifically inhibited endopolygalacturonase (PG, EC 3.2.1.15) from Botryosphaeria dothidea, was purified from B. dothidea infected apple (Malus domestica cv. Fuji) fruits. The apple PGIP was a mixed-type inhibitor of PG from B. dothidea. Optimal temperature for the maximum enzyme activity was $40^{\circ}C$, and optimum pH of the purified PGIP was pH 5.0. PGIP was stable up to temperature of $60^{\circ}C$ and was completely suppressed after heating at $70^{\circ}C$ for 10 min, PGIP was stable at pH between 4 and 8. Inhibition of PG by PGIP was reduced by $K^+$, $Cu^{2+}$, $Mg^{2+}$, $Ca^{2+}$ and $Zn^{2+}$ metal ion, sodium dodecyl sulfate (SDS) and 1,2-diaminocyclohexane tetra acetate (CDTA).

사과 겹무늬썩음병균이(Botryosphaeria dothidea) 생성하는 세포벽 분해효소인 endopolygalaturonase를 억제하는 polygalacturonase-inhibiting protein (PGIP)를 사과 과실로부터 분리하였다. 분리되어진 사과 PGIP는 사과 겹무늬썩음병균이 생성하는 PG에 대하여 혼합형의 저해를 나타내었다. PGIP의 반응 최적온도는 $40^{\circ}C$이며 최적 pH는 5.0이었다. 이 효소는 $60^{\circ}C$까지는 비교적 안정하였으나 $70^{\circ}C$에서는 효소의 활성이 완전히 억제되었으며 pH 4.0에서 8.0까지는 안정하였다. PGIP는 $K^+$, $Cu^{2+}$, $Mg^{2+}$, $Ca^{2+}$ 과 $Zn^{2+}$ 등의 금속이온과 SDS 그리고 CDTA에 의해 효소의 활성이 저해되었다.

Keywords

References

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