Effect of Samultanggamibang of Apoptosis of Melanoma cell

사물탕(四物湯) 가미방(加味方)이 흑색중(黑色腫) 세포고사(細胞枯死)에 미치는 효과(效果)

  • Park, Eun-Jung (Department of Pediatrics, college of Oriental Medicine, Wonkwang University) ;
  • Lee, Hai-Ja (Department of Pediatrics, college of Oriental Medicine, Wonkwang University) ;
  • Chang, Sung-Jin (Department of Pediatrics, college of Oriental Medicine, Wonkwang University)
  • 박은정 (원광대학교 한의과대학 소아과학 교실) ;
  • 이해자 (원광대학교 한의과대학 소아과학 교실) ;
  • 장성진 (원광대학교 한의과대학 소아과학 교실)
  • Published : 2006.04.30

Abstract

Objective : In this study, the ability of Oriental medicine Samultanggamibang(SMTG) to induce apoptosis was investigated in B16F10 melanoma cells. Method : Tetrazolium-based colorimetric assay was performed for cytotoxicity test. Several new assays for the basis of biochemical events associated with apoptosis such as DNA fragmentation by a flow cytometry, caspase-3 activation and PARP cleavage by Western blotting should be carried out potentially useful for the basis of biochemical events associated with apoptosis such as a flow cytometry and caspase-3 activation. Results : (1) The number of B16F10 melanoma cells was less than 30 % after exposure to 1 mg/ml SMTG for 48 h. SMTG increased cytotoxicity of B16F10 melanoma cells in a dose- and time-dependent manner. (2) The percentage of apoptotic cells by flow cytometric analysis of the DNA-stained cells increased to 21 % at 24 h and 25 % at 48 h after treatment with 1 mg/ml SMTG. (3) SMTG-induced apoptosis was accompained by the activation of caspase-3 and the specific proteolytic cleavage of poly-ADP-ribose polymerase. (4) SMTG induces the activation of caspase-3 and the specific proteolytic cleavage of poly-ADP-ribose polymearse and eventually leads to apoptosis through c-Jun NH2-terminal protein kinase (JNK)-dependent manner in B16F10 melanoma cells. Conclusion : SMTG had a strong cytotoxic effect of B16F10 melanoma cells.

Keywords