The Journal of Internal Korean Medicine (대한한방내과학회지)
- Volume 27 Issue 2
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- Pages.394-406
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- 2006
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- 1226-9174(pISSN)
Interleukin-5 Inhibition Assay of the Oriental Materia Medica Treatment by High Through Screening on the Splenocyte of Mouse
High through screening(HTS)를 이용한 백서(白鼠)의 비장세포(脾臟細胞)에서의 IL-5 분비 조절 한약물(韓藥物) Screening
- Park, Dong-Hee (Division of Allergy & Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyunghee University) ;
- Rhee, Hyung-Koo (Division of Allergy & Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyunghee University) ;
- Jung, Sung-Ki (Division of Allergy & Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyunghee University) ;
- Jung, Hee-Jae (Division of Allergy & Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyunghee University)
- 박동희 (경희대학교 한의과대학 폐계내과학교실) ;
- 이형구 (경희대학교 한의과대학 폐계내과학교실) ;
- 정승기 (경희대학교 한의과대학 폐계내과학교실) ;
- 정희재 (경희대학교 한의과대학 폐계내과학교실)
- Published : 2006.06.30
Abstract
Backgrounds and Objectives: Asthma is considered to be an inflammatory disease characterized by airway hyperresponsiveness and Pulmonary eosinophilia. And it is known the structure and function of IL-5, its receptor and the mechnism IL-5 triggered eosinophil accumulation and inflammaion of the airways. At this point of view, we assume which oriental materia medics can the splenocyte inhibit from secreting the IL-S in vitro. Material and Methosds: We used the splenocyte of mouse 8 weeks after its birth, and then cnltivated those into the 2 experimental groups and control group for 48 hours. The culture medium of experimental groups were made of