Journal of Acupuncture Research
- 제23권2호
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- Pages.91-102
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- 2006
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- 2586-288X(pISSN)
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- 2586-2898(eISSN)
Inhibitory Action of Ulmus Davidiana Planch Extract Solution to Osteoclast Cell Proliferation and Prostaglandin E2 Synthesis in Mice
- Park, Sang-Dong (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
- Kim, Kap-Sung (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
- Cho, Hyun-Seok (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
- Lee, Seung-Deok (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
- Kim, Kyung-Ho (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University)
- 발행 : 2006.04.20
초록
Objectives : Ulmus davidiana Planch (UD) has long been known to have anti-inflammatory and protective effects on damaged tissue, inflammation and bone among other functions. Methods : This study was undertaken to address whether the water extract of the bark of UD could modulate proliferation of mouse osteoclasts in vitro and to investigate its effect on cyclooxygenase-2 (COX-2), which converts arachidonic acid to prostaglandin E2 (PGE2) and is highly expressed in osteoclasts. Mouse osteoclasts were tested in vitro for growth inhibition, proliferation cell nuclear antigen expression, and COX-2 activity and expression after treatment with UD extract. Results : Its effects were compared with those of indomethacin (a nonselective COX inhibitor) and celecoxib (a selective COX-2 inhibitor) by Cell viability assay, Cell cycle analysis, Immunohistochemical analysis of PCNA expression, Western blot analysis and PGE2 Enzyme immunoassay (EIA). UD demonstrated a strong growth inhibitory action in both tested osteoclasts cells. The IC50s were