Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Effect of Ginseng Radix Rubra Herbal-acupuncture Solution(GRR-HAS) on Gene Expression in SNU484 carcinomar cells

홍삼약침액(紅蔘藥鍼液)의 위암세포주(胃癌細胞柱) 유전자(遺傳子) 발현(發顯)에 미치는 영향(影響)

  • Won, Eun-Ju (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University) ;
  • Lee, Kyung-Min (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University) ;
  • Lee, Bong-Hyo (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University) ;
  • Lim, Seong-Chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University) ;
  • Jung, Tae-Young (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University) ;
  • Seo, Jung-Chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University)
  • 원은주 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이경민 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이봉효 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 임성철 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 정태영 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 서정철 (대구한의대학교 한의과대학 침구경혈학교실)
  • Published : 2006.06.30
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Abstract

Objective : It has long been known about the anticancer effect of GRR-HAS, however, it has not been systemically determined the differentially regulated genes by GRR-HAS in cancer cells. The purpose of this study is to screen the GRR-HAS mediated differentially expressed genes in cancer cells such as SNU484 gastric cancer cell lines. Oligonucleotide microarray approache was employed to screen the differential expression genes. Methods : GRR-HAS was prepared by boiling and stored at $-70^{\circ}C$ until use. Cells were treated with various concentrations of GRR-HAS(0.1, 0.5, 1.5, 10, 20mg/ml) for 24 h. Cell toxicity was tested by MTT assay. To screen the differentially expressed genes in cancer cells, cells were treated with 1.5mg/ml of GRR-HAS. For oligonucleotide microarray assay, total RNA was used for gene expression analysis using oligonucleotide Genechip (Human genome Ul33 Plus 2.0., Affimatrix Co.). Results : It has no cytotoxic effects on both HepG2 and SNU484 cells in all concentrations(0.1, 0.5, 1.5, 10, 20mg/ml). In oligonucleotide microarray assay, in SNU484 cells, the number of more than twofold up-regulated genes was 346. The number of more than twofold down-regulated genes was 9. Discussion : This study showed the comprehensive gene expression analysis using oligonucleotide microarray for the screening of GRR-HAS mediated differentially regulated genes. These results will provide a better application of GRR-HAS in cancer field and drug target development.

Keywords

References

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