The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of Quercus dentata and Quercus acutissima Extracts on the Activations of Macrophage RAW264.7 Primed with $IFN-{\gamma}$

곡피(?皮)와 상목피(橡木皮) 추출물이 대식세포 RAW264.7 활성화에 미치는 영향

  • Jo, Jin-Hee (Dept. of Herbology, College of Oriental Medicine, Kyungwon University) ;
  • Seong, Nak-Sull (National Crop Experiment Station, RDA) ;
  • Lee, Young-Jong (Dept. of Herbology, College of Oriental Medicine, Kyungwon University)
  • 조진희 (경원대학교 한의과대학 본초학교실) ;
  • 성낙술 (농촌진흥청 작물과학원) ;
  • 이영종 (경원대학교 한의과대학 본초학교실)
  • Published : 2006.03.30
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Abstract

Objectives : The effects of methanol extracts from the cortex of Quercus dentata Thunb. and Quercus acutissima Carruth, on the activation of macrophage were examined. Methods : Methanol extracts of Quercus dentata (QD) and Quercus acutissima (QA) were applied to cell line RAW264,7 (macrophage), and their effects were examined. Results : 1. Extracts from QD and QA had no specific influence on the cell growth. 2. Extracts from QD and QA did not activate macrophage independently, but the addition of $IFN-{\gamma}$ facilitated the generation of macrophage's nitric oxide(NO). 3. QD and QA extracts increased the manifestation of iNOS gene, when macrophage was activated by $IFN-{\gamma}$. 4. QD and QA extracts increased the manifestation of $TNF-{\alpha}$ in macrophage, which took 2 hours. 5. QD and QA extracts increased the generation of $PGE_2$ in macrophage. Conclusion : QD and QA activate macrophage in the presence of $IFN-{\gamma}$. After activation is primarily facilated by $IFN-{\gamma}$, it works on macrophage secondarily for the manifestation of iNOS gene and for the generation of $TNF-{\alpha}$.

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