Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Allopurinol on Vascular Endothelial Cells Damaged by Hydrogen Peroxide In Vitro

Hydrogen Proxide에 의해 손상된 배양 혈관내피세포에 대한 Allopurinol의 영향

  • Suk, Seung-Han (Department of Neurology, Wonkwang University Sanbon Medical Center)
  • 석승환 (원광대학교 의과대학 산본병원 신경과학교실)
  • Published : 2006.08.25
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Abstract

In order to examine the effect of oxygen free radicals on the vascular endothelial cells, cell viability was measured by XTT assay after bovine pulmonary vascular endothelial cell line(BPVEC) was treated only with hydrogen peroxide. In addition, the antioxidant effect of allopurinol on cells treated with hydrogen peroxide was examined by colormetric assay. in this study, the BPVEC treated with hydrogen peroxide showed the significantly decreased cell viability compared with control. Whereas, the viability of cells treated with hydrogen peroxide and allopurinol has significantly increased when compared with that of cells treated only with hydrogen peroxide. These results suggested that hydrogen peroxide, one of the oxygen free radicals showed cytotoxic effect and allopurinol has protective effect on oxygen free radical-induced cytotoxicity.

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References

  1. Libby, P., Deanfiled, J.E. A CME monograph : Atherosclerosis and vascular disease Academy for Health Education. pp 1-28, 2002
  2. Sellke, F.M., Amstrong, M.L., Harrison, D.G. Endothelium- dependent vascular relaxation is abnormal in the coronary microcirculation of atheosclerotic primates. Circ Res 81:1586-1593, 1990 https://doi.org/10.1161/01.CIR.81.5.1586
  3. Ballinger, S.W., Patterson, C., Yan, C.N., et al. Hydrogen peroxide- and peroxynitrite-induced mitochondrial DNA and dysfunction in vascular endothelial and smooth muscle cells Circ Res. 86:960-966, 2000 https://doi.org/10.1161/01.RES.86.9.960
  4. Bevilacqua, M.P. Endothelial-leukocyte adhesion molecules. Annu Rev Immunol 11:767-804, 1993 https://doi.org/10.1146/annurev.iy.11.040193.004003
  5. Toda, N., Okamura, T. Role of nitric oxide in neurally induced cerebroarterial relaxation. J Pharmacol Exp Ther 258:1027-1032, 1991
  6. Harrison, D.G. Endothelial dysfunction in atherosclerosis. Basic Res Cardiol 89(suppl):87-102, 1994
  7. Kaneko, M., Elmban, V., Dhalla, N.S. Mechanism for depression of heart sarcolemmal $Ca^{2+}$ pump by oxygen free radicals. Am J Physiol 261:4948-4955, 1989
  8. Toraason, M., Breitenstein, M. Intercellular calcium transients in cardiac myocytesexposed to carbon tetrachloride(Abstract). Toxicologyist 11:310, 1991
  9. Gibbons, G.H., Dzau, V.J. Molecular therapies for vascular diseases. Science 272:689-693, 1996 https://doi.org/10.1126/science.272.5262.689
  10. Johnstone, M.T., Creager, S.J., Scales, K.M. et al. Impaired endothelium-dependent vasodilation in patients with insulin-dependent diabetes mellitus. Circulation 88:2510-2516, 1993 https://doi.org/10.1161/01.CIR.88.6.2510
  11. Tesfamariam, B. Free radicals in diabetic endothelial cell dysfunction. Free Radic Biol Med 16:383-391, 1994 https://doi.org/10.1016/0891-5849(94)90040-X
  12. Gawler, D., Milligan, G., Spiegel, A.M., et al. Abolition of the expression of inhibitory guanine nucleotide regulatory protein Gi activity in diabetes. Nature 327:229-232, 1987 https://doi.org/10.1038/327229a0
  13. Greene, D.A., Lattimer, S.A., Sima, A.A.F. Sorbitol, phosphoinositides, and sodium-ATPase in the pathogenesis of diabetic complication. N Engl J Med 316:599-606, 1987 https://doi.org/10.1056/NEJM198703053161007
  14. Lee, T.S., MacGregor, L.C., Fluharty, S.L. et al. Differential regulation of protein C and (Na, K)-adenosine triphosphate activities by elevated glucose levels of retinal capillary endothelial cells. J Clin Invest 83:90-94, 1989 https://doi.org/10.1172/JCI113889
  15. Ganz, M.B., Saksa, B., saxena, R., Hawkins, K., Sedor, J.R. PDGF and IL-1 induce and activate specific protein kinase C isoforms in mesangial cells. Am J Physiol 217:F108-F113, 1996
  16. Yan, S.D., Schmidt, A.M., Anderson, G.M. Zhang, J., Brett, J., Zou, Y.S., Pinsky, D., Stern, D. Enhansed cellular oxidant stress by the interaction of advanced glycosylation end products with their receptors/binding proteins. J Biol Chem 269:9889-9897, 1994
  17. Dario, G., Antonio, C., Giuseppe, P. Oxidative stress and diabetic vascular complications. Diabetes Care 19:257-267, 1996 https://doi.org/10.2337/diacare.19.3.257
  18. Sawada, M., Kondo, N., Suzumura, A., Marunouchi, T. Production of tumor necrosis factor-alpha by microglia and astrocytes in culture. Brain Res 491:394-397, 1989 https://doi.org/10.1016/0006-8993(89)90078-4
  19. Goldstein, R.H., Poliks, C.F., Pilch, P.F., Smith, B.D. Fine A. Stimulation of collagen formation by insulin and insulin-like growth factor I in cultures of human lung fibroblasts. Endocrinology 124(2):964-970, 1989 https://doi.org/10.1210/endo-124-2-964
  20. Mosmann, T. Rapid colorimetric assay for cellular growth and survival : Application to proliferation and cytotoxic assays. J Immunol Methods 65:55-63, 1983 https://doi.org/10.1016/0022-1759(83)90303-4
  21. Borenfreund, E., Babichi, H., Matin-Alcuacil, N. Comparisons of two in vitro cytotoxicity assay. The neutral red(NR) and tetrazolium MTT tests. Toxicol In Vitro 2:1-8, 1988 https://doi.org/10.1016/0887-2333(88)90030-6
  22. Loft, S., Astrup, A., Buemann, B., Poulsen, H.E. Oxidative DNA damage correlates with oxygen consumption in humans. FASEB J 8:534-537, 1994 https://doi.org/10.1096/fasebj.8.8.8181672
  23. Elion, G.B., Kovensky, A., Hitchings, G.H., Metz, E., Rundles, R.W. Metabolic studies of allopurinol, an inhibitor of xanthine oxidase. Biochem Pharmacol pp 863-880, 1996
  24. Mayer, M.L., Westbrook, G.L. Permeation and block of N-methyl-D-aspartic receptor channels by divalent cations in mouse cultured central neurons. J Physiol pp 501-527, 1987
  25. Tesfamariam, B., Cohen, R.A. Free radicals mediate endothelial cell dysfunction caused by elevated glucose. Am J Physiol 263:H321-H326, 1992