동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제20권4호
  • /
  • Pages.936-941
  • /
  • 2006
  • /
  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

고장초의 부위별(뿌리, 줄기, 전초) Neuro2A 신경세포고사에 대한 억제 효과 비교 연구

Comparative Study on the Inhibition Effect on Apoptosis in Neuro2A Cell on the Region of Zizania Latifolia(Radix, Rhizoma, Herba)

  • 차윤엽 (상지대학교 한의과대학 한방재활의학과)
  • Cha, Yun-Yeop (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Sangji University)
  • 발행 : 2006.08.25
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

To prevent human body injury from oxidative stress, antioxidants are very important and many research about antioxidants are generally being conducted. Hydrogen peroxide$(H_20_2)$ that is one of vitality oxygen species has been seen that cause various diseases, DNA damage and gene change. We have already known that the inhibition effect of Zizania latifolia Radix, Rhizoma on apoptosis induced by $H_2O_2$ in Neuro2A cell. And the purpose of this study was that we made a comparative study on the inhibition effect of apoptosis in Neuro2A cell on the region of Zizania latifolia(Radix, Rhizoma, Herba). Neuro2A cells were cultivated in RPMI(GibcoBRL) with 5% FBS and treated with $H_2O_2$ and Zizania latifolia(Radix, Rhizoma, Herba). Separately we measured the cell viability and analyzed DNA fragmentation. Activity of PARP, Cytochrome C, caspase-9, caspase-3, p53, p21, Bax and Bcl-2 in the cell was examined by using western blot. The results obtained were as Follows: The cell viability in all of Zizania latifolia (Radix, Rhizoma, Herba) treatment (60ug/m1<) decreased significantly compared with that of none treatment(p<0.001). Zizania latifolia Radix increased cell viability was most effective of three regions. But we had no significant difference among three regions. All of Zizania latifolia (Radix, Rhizoma, Herba) increased cell viability about twice as much as that being injury by $H_2O_2$,(Zizania Latifolia (Radix, nhizoma, Herba) 20ug/m1, $H_2O_2$ 200uM, p<0.001). DNA fragmentation developed by $H_2O_2$, but was not developed in all of Zizania latifolia (Radix, Rhizoma, Herba) treatment. PARP, Cytochrome C, caspase-9 and caspase-3 activated all by $H_2O_2$ but were not activated in all of Zizania latifolia (Radix, Rhizoma, Herba) treatment. P53, P2l and Bax activated by $H_2O_2$, and Bcl-2 got into inactivation. But the opposite results appeared in all of Zizania latifolia (Radix, Rhizoma, Herba) treatment. In conclusion, these results suggest that all of Zizania latifolia (Radix, Rhizoma, Herba) inhibit the development of DNA fragmentation and apoptosis by $H_2O_2$and the antioridant action of all of Zizania latifolia (Radix, Rhizoma, Herba) is effective.

키워드

참고문헌

  1. 두호경 편저. 동의신계학. 서울, 동양의학연구원. pp 1327- 1329, 1993
  2. Cohen, G. The generation of hydroxyl radicals in biological system. Photobiol. 28, 669-675, 1978 https://doi.org/10.1111/j.1751-1097.1978.tb06993.x
  3. Halliwell, B., Gutteridge, J.M. The chemistry of oxygen radicals and other oxygen-derived species. In free radicals in biology and medicine. 2nd Ed. Oxford : Clarendon Press. pp 22-31, 1989
  4. Halliwell, B., Gutteridge, J.M. Protection against oxidants in biological systems : the superoxide theory of oxygen toxicity. In free radicals in biology and medicine. 2nd Ed. Oxford : Clarendon Press. pp 86-89, 1989
  5. Hertog, M.G.L., P.C.H. Hollman. Potential health effects of the dietary flavonid quercetin, Eur. J. Clin. Nutr. 50(2):63-66, 1996
  6. Benavente-Garcisa, O., J.C. Marrin, F.R. Ortuno, A. Rio. Uses and properties of citurs flavonoids, J. Agric. Food Chem. 45, 4505-4515, 1997 https://doi.org/10.1021/jf970373s
  7. 尹國炳, 張俊根. 몸에 좋은 山野草. 서울, 石悟出版社. p 410, 1989
  8. 中藥大辭典. 서울, 醫聖堂. p 2052, 1994
  9. 동식, 김근하, 김영호, 최승문. 고려치료경험 내과편. 의학과학출판사. p 71, 76, 114, 122, 132, 166, 345, 386, 1996
  10. 박원형. $H_2O_2$로 유발된 Neuro2A 신경세포고사에 대한 줄풀(Zizania latifolia)의 억제 효과. 상지대학교 석사학위논문. 2005
  11. 차윤엽. 줄풀 줄기의 Neuro2A 신경세포고사에 대한 억제 효과. 동의생리병리학회지 20(1):149-155, 2006
  12. 고경식. 가을에 꽃 피는 야생식물. 서울, 일진사. p 56, 2004
  13. 동식, 김근하, 김영호, 최승문. 고려치료경험 내과편. 의학과학출판사. p 71, 76, 114, 122, 132, 166, 345, 386, 1996
  14. http://www.allfull.co.kr. 2004년 6월 12일
  15. 최진규. 약이 되는 우리풀.꽃.나무2. 서울, 한문화. pp 141-147, 2003
  16. 李時珍. 本草綱目. 北京, 人民衛生出版社. p 1366, 1982
  17. 許浚. 對譯 東醫寶鑑. 서울, 법인문화사. p 1956, 1999
  18. 李梴. 編注 醫學入門. 서울, 醫聖堂. p 331, 1994
  19. 中國藥材公司編著. 中國中藥資源志要. 北京, 科學出版社. p 1460, 1994
  20. 寇宗奭. 本草衍義. 서울, 醫聖堂. p 74, 1994
  21. Su-Noh Ryu, Sun-Zik Park, Hong-Yeol Kim. Antioxidant Activity in Rice Cultivar, Wild Rice, and Barley. Korean J. Crop Sci. 47(1):54-61, 2002
  22. Li, P., Nijhawan, D., Budihardjo, I., Srinivasula. S.M, Ahmad, M., Alnemri, E.S., et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell. 91(4):479-489, 1977
  23. Soldani, C., Scovassi, A.I. Poly(ADP-ribose) polymerase-1 cleavage during apoptosis. an update. Apoptosis. 7(4):321-328, 2002 https://doi.org/10.1023/A:1016119328968
  24. Bert T, Kenneth WK. P53 function and dysfunction. Cell. 70, 523-526, 1995
  25. Lane, D.P. A death in the life of P53. Nature. 362(6423): 849-852, 1993 https://doi.org/10.1038/362849a0
  26. 안원근. 노화과정에 있어서 P21 유전자의 역할. 생화학분자생물학뉴스. 한국생화학분자생물학회. 23(2):80-81, 2003
  27. Yang, E., Korsmeyer, S.K. Molecular thanatosis: A discourse on the bcl-2 family and cell death. Blood. 88(2):386-401, 1996.
  28. Oltvai, Z., Milliman, C., Korsmeyer, S.J. bcl-2 heterodimerizes in vivo with a conserved homolog, Bax that accelerates programmed cell death. Cell. 74(4):609-619, 1993 https://doi.org/10.1016/0092-8674(93)90509-O