Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Immunomodulatory Effects of Aqueous-extracted Zingiberis rhizoma on Methorexate Induced Immune Suppression in Mouse Spleen Cell

건강 열수추출액이 Methotrexate에 의해 유도된 마우스 면역억제 조절에 미치는 영향

  • Lee, Young-Sun (Department of Traditional Health Resource Development, College of Sung Duk) ;
  • Lee, Geum-Hong (Department of Pathology, College of Oriental Medicine, Daegu Haany University) ;
  • Kim, Sang-Chan (Department of Prescription, College of Oriental Medicine, Daegu Haany University) ;
  • Kwon, Young-Kyu (Department of Physiology, College of Oriental Medicine, Daegu Haany University) ;
  • Shin, Sang-Woo (Department of Pathology, College of Oriental Medicine, Daegu Haany University)
  • 이영선 (성덕대학 전통건강자원개발과) ;
  • 이금홍 (대구한의대학교 한의과대학 병리학교실) ;
  • 김상찬 (대구한의대학교 한의과대학 방제학교실) ;
  • 권영규 (대구한의대학교 한의과대학 생리학교실) ;
  • 신상우 (대구한의대학교 한의과대학 병리학교실)
  • Published : 2006.08.25
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Abstract

Zingiberis rhizoma(ZB) has been used to treat a various condition and disease in traditional oriental medicine. The present study was conducted to evaluate the immunomodulatory effect of aqueous-extracted ZB(ZBE) on methotrexate (MTX)-induced immune suppression in mouse spleen cells. In spleen cell proliferation assay, ZBE enhanced mitogenic activity in mouse spleen cells. In RT-PCR, ZBE induced IL-2, IFNr and IL-6 cytokine gene expression in mouse spleen cells. In spite of MTX treatment, IL-2, IFNr and IL-6 gene expressions sustained in MTX treated spleen cells. CD45R/B220, pan B marker was slightly increased in ZBE treated mouse spleen cells. IL-6, B cell tropical cytokine, production was induced by ZBE-treated mouse spleen cells and IL-6 production was sustained on MTX-ZBE co-cultured cells. ZBE administration enhanced suNival of S-180 bearing mouse. These data indicate that ZBE has a protective effect of immune suppression caused by MTX, and ZBE may be enhance cellular and humoral function by regulate cytokine gene expression as well as the mitogenic effect on spleen cells.

Keywords

References

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