Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Relationship of Inhibitory Effects of Dichroa febrifuga and $IKK{\gamma}$ on the Activation of $NF-{\kappa}B$

상산의 $NF-{\kappa}B$ 활성억제작용과 $IKK{\gamma}$의 연관성 연구

  • Choi, Byung-Tae (Department of Anatomy, College of Oriental Medicine, Dongeui University) ;
  • Lee, Yong-Tae (Department of Physiology, College of Oriental Medicine, Dongeui University) ;
  • Hwang, Jang-Sun (Department of Microbiology, College of Natural Sciences, Changwon National University) ;
  • Moon, Hae-In (Department of Microbiology, College of Natural Sciences, Changwon National University) ;
  • Lee, Kyung-Soo (Department of Microbiology, College of Natural Sciences, Changwon National University) ;
  • An, Won-Gun (Department of Nanomedical engineering, College of Nanobiotechnology, Pusan National University) ;
  • Kim, Dong-Wan (Department of Microbiology, College of Natural Sciences, Changwon National University)
  • 최병태 (동의대학교 한의과대학 해부학교실) ;
  • 이용태 (동의대학교 한의과대학 생리학교실) ;
  • 황장선 (창원대학교 자연과학대학 미생물학과) ;
  • 문혜인 (창원대학교 자연과학대학 미생물학과) ;
  • 이경수 (창원대학교 자연과학대학 미생물학과) ;
  • 안원근 (부산대학교 나노과학기술대학 나노메디컬 공학과) ;
  • 김동완 (창원대학교 자연과학대학 미생물학과)
  • Published : 2006.06.25
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Abstract

Activation of $NF-{\kappa}B$ is known to be a trigger of various cellular disorders including inflammatory and autoimmune diseases such as rheumatoid arthritis. Numerous approaches are ongoing within laboratories to identify potential therapeutic agents which inhibit the $NF-{\kappa}B$ activation. In this study, we have tested the inhibitory effects of five traditional medicines on the activation of $NF-{\kappa}B$ by NIK. Among three medicines which exhibited inhibitory effect on the expression of $NF-{\kappa}B$ repoter plasmid, we investigated further the inhibitory mechanism of Dichroa febrifuga in connection with IKKY activity. Wild type $IKK{\gamma}$ inhibited the $NF-{\kappa}B$ activation by NIK but the C-terminal deletion mutant of IKKY did not show the inhibitory effect, indicating that the C-terminal leucine zipper domain of $NF-{\kappa}B$ is important for the inhibition of $NF-{\kappa}B$ activation. The water extract of Dichroa febrifuga(DFE) also strongly inhibited the $NF-{\kappa}B$ activation by NIK. The inhibitory activity of DFE appeared to be independent of the expression of $IKK{\gamma}$, suggesting that the pathways of inhibition by Dichroa febrifuga and $IKK{\gamma}$ are different. Our results suggest that Dichroa febrifuga can be used as a medicine for inhibition of the $NF-{\kappa}B$ activation in a wide range of cells without relation to the expression of $IKK{\gamma}$.

Keywords

References

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