Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Water Extracts from Chaenomeles sinensis, Polygonum cuspidatum and Boswellia carterii on LPS-Induced Nitric Oxide Production in Raw 264.7 Cell

목과(木瓜), 호장근(虎杖根) 및 유향(乳香) 추출물이 Raw 264.7 cell에서 LPS로 유도된 nitric oxide 생성에 미치는 영향

  • Lee, Tae-Jin (Department of Immunology, School of Medicine, Keimyung University) ;
  • Woo, Kyung-Jin (Department of Immunology, School of Medicine, Keimyung University) ;
  • Shu, Seong-Il (Department of Microbiology, School of Medicine, Keimyung University) ;
  • Shin, Sang-Woo (College of Oriental Medicine, Daegu Hanny University) ;
  • Kim, Sang-Chan (College of Oriental Medicine, Daegu Hanny University) ;
  • Kwon, Young-Kyu (College of Oriental Medicine, Daegu Hanny University) ;
  • Park, Jong-Wook (Department of Immunology, School of Medicine, Keimyung University) ;
  • Kwon, Taeg-Kyu (Department of Immunology, School of Medicine, Keimyung University)
  • 이태진 (계명대학교 의과대학 면역학교실) ;
  • 우경진 (계명대학교 의과대학 면역학교실) ;
  • 서성일 (계명대학교 의과대학 미생물학교실) ;
  • 신상우 (대구한의대학교 한의과대학) ;
  • 김상찬 (대구한의대학교 한의과대학) ;
  • 권영규 (대구한의대학교 한의과대학) ;
  • 박종욱 (계명대학교 의과대학 면역학교실) ;
  • 권택규 (계명대학교 의과대학 면역학교실)
  • Published : 2006.06.25
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Abstract

In activated macrophage, large amounts of nitric oxide (NO) are generated by inducible nitric oxide synthase (iNOS), resulting in acute or chronic inflammatory disorders. In Raw 264.7 cells stimulated with lipopolysaccharide (LPS) to mimic inflammation, water extracts from the fruit of Chaenomeles sinensis, the root of Polygonum cuspidatum and Boswellia carterii inhibited the LPS-induced NO production in a parallel dose-dependent manner. To investigate the mechanism by which those extracts inhibits NO production, we examined the expression of iNOS and COX-2 in both mRNA and protein levels. We observed a significant change in the iNOS expression between LPS alone and LPS plus those extracts-treated cells. However, water extracts from Chaenomeles sinensis, Polygonum cuspidatum and Boswellia carterii did not inhibit COX-2 expression which was induced by LPS treatment in Raw 264.7 cells. These data suggest that water extracts from Chaenomeles sinensis, Polygonum cuspidatum and Boswellia carterii can modulate anti-inflammatory immune response, which may be in part associated with the regulation of NO synthesis through the regulation of iNOS expression in mouse macrophage cells.

Keywords

References

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