Bioinformatics and Biosystems
- Volume 1 Issue 1
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- Pages.67-72
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- 2006
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- 1738-9798(pISSN)
Effect of missing values in detecting differentially expressed genes in a cDNA microarray experiment
- Kim, Byung-Soo (Dept of Applied Statistics, Yonsei University) ;
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Rha, Sun-Young
(Cancer Metastasis Research Center, College of Medicine, Yonsei University, Brain Korea 21 Project for Medical Science, College of Medicine, Yonsei University)
- Published : 2006.02.28
Abstract
The aim of this paper is to discuss the effect of missing values in detecting differentially expressed genes in a cDNA microarray experiment in the context of a one sample problem. We conducted a cDNA micro array experiment to detect differentially expressed genes for the metastasis of colorectal cancer based on twenty patients who underwent liver resection due to liver metastasis from colorectal cancer. Total RNAs from metastatic liver tumor and adjacent normal liver tissue from a single patient were labeled with cy5 and cy3, respectively, and competitively hybridized to a cDNA microarray with 7775 human genes. We used
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