Bulletin of the Korean Chemical Society

  • 제27권3호
  • /
  • Pages.407-412
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  • 2006
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  • 0253-2964(pISSN)
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  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
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Bioluminescence Single-Site Immunometric Assay for Methamphetamine Using the Photoprotein Aequorin as a Label

  • 발행 : 2006.03.20
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초록

Bioluminescence single-site immunometric assay for methamphetamine (MA) using the native aequorin, a photoprotein, as a signal generator was developed for the first time. MA is a potent sympathomimetic amine with stimulant effects on the central nervous system. MA abuse induces hallucinations and, thus, may cause a serious social problem. The single-site immunometric MA assay was optimized and its dose-response behavior was examined. The dose-response curve shows that the detection limit is 1.1 ${\times}$ $10^{-10}$ M and a dynamic range is four orders of magnitude with 15 $\mu$g/mL BSA-MA conjugate and 1.0 ${\times}$ $10^{-8}$ M anti-MA antibody-biotin conjugate. In order to evaluate this assay, the structurally similar compounds, amphetamine, ephedrine, norephedrine, benzphetamine and N-4-(aminobutyl)methamphetamine were examined for their crossreactivity. None of these five compounds showed any cross-reactivity. Additionally, an artificial urine solution spiked with MA was analyzed by the MA assay, and the result of the analysis demonstrated the usefulness of the present assay for the determination of MA in urine.

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피인용 문헌

  1. Competitive enzyme-linked immunosorbent assay for a selective and sensitive determination of dopamine in the presence of ascorbic acid and uric acid. vol.31, pp.1, 2006, https://doi.org/10.1080/15321810903404988
  2. Development of direct competitive enzyme-linked aptamer assay for determination of dopamine in serum vol.685, pp.1, 2006, https://doi.org/10.1016/j.aca.2010.11.010