Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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High-Performance Liquid Chromatographic-Tandem Mass Spectrometric Determination of Itraconazole in Human Plasma for Bioavailability and Bioequivalence Studies

  • Choi, Young-Wook (Division of Pharmaceutical Sciences, College of Pharmacy, Chung-Ang University) ;
  • Nam, Dae-young (Division of Pharmaceutical Sciences, College of Pharmacy, Chung-Ang University) ;
  • Kang, Kyoung-Hoon (Division of Pharmaceutical Sciences, College of Pharmacy, Chung-Ang University) ;
  • Ha, Kyung-Wook (Division of Pharmaceutical Sciences, College of Pharmacy, Chung-Ang University) ;
  • Han, In-Hee (Division of Pharmaceutical Sciences, College of Pharmacy, Chung-Ang University) ;
  • Chang, Byung-Kon (Division of Pharmaceutical Sciences, College of Pharmacy, Chung-Ang University) ;
  • Yoon, Mi-kyeong (Division of Pharmaceutical Sciences, College of Pharmacy, Chung-Ang University) ;
  • Lee, Jae-hwi (Division of Pharmaceutical Sciences, College of Pharmacy, Chung-Ang University)
  • Published : 2006.02.20
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Abstract

A highly sensitive high-performance liquid chromatographic-tandem mass spectrometric method (HPLC-MSMS) has been developed to quantify itraconazole in human plasma for the purpose of pharmacokinetic studies. Sample preparation was carried out by liquid-liquid extraction using loratadine as an internal standard. Chromatographic separation used a YMC $C_{18}$ column, giving an extremely fast total run time of 3 min. The method was validated and used for the bioequivalence study of itraconazole tablets in healthy male volunteers (n = 31). The lower limit of detection proved to be 0.2 ng /mL for itraconazole.

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