한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)
- 제51권spc1호
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- Pages.269-273
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- 2006
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- 0252-9777(pISSN)
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- 2287-8432(eISSN)
대량의 쌀 시료 분석을 위한 DNA 추출법
High-Throughput DNA Extraction Method for Marker Analysis in Rice Grain
- 최영덕 (다카라코리아바이오메디칼(주) 연구개발센터) ;
- 이해광 (다카라코리아바이오메디칼(주) 연구개발센터) ;
- 이윤숙 (다카라코리아바이오메디칼(주) 연구개발센터) ;
- 윤정희 (다카라코리아바이오메디칼(주) 연구개발센터) ;
- 김수정 (다카라코리아바이오메디칼(주) 연구개발센터) ;
- 박성환 (다카라코리아바이오메디칼(주) 연구개발센터)
- Choi, Young-Deok (Division of R&D Center, TakaraKoreaBiomedical Inc.) ;
- Lee, Hae-Kwang (Division of R&D Center, TakaraKoreaBiomedical Inc.) ;
- Lee, Yun-Suk (Division of R&D Center, TakaraKoreaBiomedical Inc.) ;
- Yun, Jeong-Hee (Division of R&D Center, TakaraKoreaBiomedical Inc.) ;
- Kim, Su-Jeong (Division of R&D Center, TakaraKoreaBiomedical Inc.) ;
- Park, Seong-Whan (Division of R&D Center, TakaraKoreaBiomedical Inc.)
- 발행 : 2006.11.30
초록
The study of molecular markers to improve crops largely depends on the availability of rapid and of efficient DNA extraction methods. Here we developed a cheap and convenient method to isolate genomic DNA from rice grains suitable for large-scale microsatellite analysis. We confirmed that the isolated rice DNA is suitable for PCR analysis with STS marker and SNP marker, as well as microsatellite marker. Further, we established high-throughput DNA extraction system in a 96-well plate format which make it possible high-throughput analysis of microsatellite markers with rice grains. This implies that the new method could be a useful tool for other types of marker analysis in large scale.