International Journal of Industrial Entomology and Biomaterials

  • 제10권1호
  • /
  • Pages.73-77
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  • 2005
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  • 3022-7542(pISSN)
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  • 2586-4785(eISSN)
한국잠사학회 (Korean Society of Sericultural Science)
권호 표지

Cloning and Characterization of the Cu,Zn Superoxide Dismutase (SOD1) cDNA from the Spider, Araneus ventricosus

  • Choi Young Soo (College of Natural Resources and Life Science, Dong-A University) ;
  • Choo Young Moo (College of Natural Resources and Life Science, Dong-A University) ;
  • Li Jianhong (College of Natural Resources and Life Science, Dong-A University, Department of Plant Protection, College of Plant Science and Technology, Hyazhong Agricultural University) ;
  • Sohn Hung Dae (College of Natural Resources and Life Science, Dong-A University) ;
  • Jin Byung Rae (College of Natural Resources and Life Science, Dong-A University)
  • 발행 : 2005.03.01
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초록

A Cu,Zn superoxide dismutase (SOD1) cDNA was cloned from the spider, Araneus ventricosus. The A. ventricosus SOD1 (AvSOD1) cDNA contains an open reading frame of 495 bp encoding 165 amino acid polypeptide with a predicted molecular mass of 17,114 Da and pI of 6.55, and possesses the typical metal binding ligands of six histidines and one aspartic acid common to SOD1s. The deduced amino acid sequence of the AvSOD1 cDNA showed $51\%$ identity to Ceratitis capitata SOD1, and $50\%$ to SOD1 sequences of both Drosophila melanogaster and Chymomyza amoena. Northern blot analysis revealed the presence of AvSOD1 transcripts in all tissues examined.

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