Computational Analysis of Apolipophorin-III in Hyphantria cunea

  • Chandrasekar R. (Department of Biotechnology, Bharathidasan University, Division of Applied Life Science, Gyeongsang National University) ;
  • Dhanalakshmi R. (Department of Biotechnology, Bharathidasan University) ;
  • Krishnan M. (Department of Biotechnology, Bharathidasan University) ;
  • Kim H. J. (Division of Applied Life Science, Gyeongsang National University) ;
  • Jeong H. C. (Hampyeong County Insect Institute) ;
  • Seo S. J. (Division of Applied Life Science, Gyeongsang National University)
  • Published : 2005.03.01

Abstract

Recently a cDNA clone of apoLp-III from Hyphantria cunea was isolated and subjected to computational analysis to compare with other available sequences. Multiple sequence alignments were carried out using the amino acid sequences of apoLp-III from six insects. It was found that the H. cunea apoLp-III has relatively high sequence identities to Spodoptera litura ($69.5\%$), Manduca sexta ($66.8\%$), Galleria mellonella ($65.1\%$), Bombyx mori N4 ($54.3\%$) but less identity to Locusta migratoria ($18.3\%$). The amino acid composition was compared with other insects using EXPASY tools; it shows that alanine (Ala), glutamine (Gln), leucine (Leu) and lysine (Lys) are the major amino acid components of apoLp-III in H. cunea as well as other lepidopterans. Homology modeling performed using PSI-BLAST (PDB template M. sexta) reveals that the apoLp-III molecules consist of five, long amphipathic alpha helical bundles with short loops connecting the helices and shows homology with other insects. Phylogenetic analysis shows that the orthopteran apoLp-III represented by locust was most distantly related to the lepidopteran insects.

Keywords

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