생명과학회지 (Journal of Life Science)

  • 제15권1호
  • /
  • Pages.141-146
  • /
  • 2005
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

NIH3T3 세포에서 UVB에 의한 세포고사에 미치는 옻 추출물과 fisetin의 효과

Effect of Rhus verniciflua Stokes Extract and Fisetin on UVB-induced Apoptosis in NIH3T3 Cells

  • Kim Don Young (Division of Biological Science, Wonkwang University) ;
  • Hwang Eun Hee (Diepartment of Food and Nutrition, Wonkwang University) ;
  • Park Jong Kun (Division of Biological Science, Wonkwang University)
  • 발행 : 2005.02.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

For a long time Rhus vemiciflua Stokes (RVS) has been traditionally used as a herbal plant in Asia. In this study, we have investigated the effect of acetone extract of Rhus verniciflua Stokes (RVSE) and fisetin, a component of RVSE, on DNA damage response in NIH3T3 cells. Exposure of cells to DVB light $(200 J/m^2)$ and postincubation in growth medium for 48 hr resulted in a decrease of cell viability to about $10-20\%$ of nontreated control. Addition of various concentrations of RVSE in the postincubation medium, however, significantly increased the cell viability as compared with the values expected. The genotoxicity-decreasing effect was also demonstrated in cells exposed to UVB light and incubated in medium containing fisetin. The genotoxicity-decreasing effect of RVSE and fisetin was further demonstrated by various analyses including cell morphology studies, trypan blue exclusion assay and DAPI staining. By Annexin V binding analysis, RVSE and fisetin were shown to decrease the early apoptosis induced by UVB exposure. These results suggest the RVSE contain components that either increase the DNA repair or decrease the apoptosis in UVB-exposed cells.

키워드

참고문헌

  1. Dashwood, R. and D. Guo. 1993. Antimutagenic potency of chlorophyIlin in the Salmonella assay and its correlation with binding constants of mutagen-inhibitors complex. Environ. Mol. Mutagenesis. 22, 164-171 https://doi.org/10.1002/em.2850220309
  2. Guengerich, F. P. 1989. Roles of cytochrome P-450 enzymes in chemical cacinogenesis and cancer chemotherapy. Cancer Res. 48, 2946-2954
  3. Harborne, J. B. and C. A. Williams. 2000. Advances in flavonoids research since 1992. Phytochemisty 55, 481-504 https://doi.org/10.1016/S0031-9422(00)00235-1
  4. Hayatsu, H., N. Inada, T. Kakutani, S. Arimoto, T. Negishi, K. Mori, T. Okuda and I. Sakata. 1992. Suppression of genotoxicity of carcinogens by (-)-epigallocatechin gallate. Prevent. Med. 21, 370-376 https://doi.org/10.1016/0091-7435(92)90044-I
  5. Hwang, S. J., J. Y. Cha, S. G. Park, G. J. Joe, H. M. Kim, H. B. Moon, S. J. Jeong, J. S. Lee, D. H. Shin, S. R. Ko and J. K. Park. 2002. Diol and triol type ginseng saponins potentiate the apoptosis of NIH3T3 cells exposed to MMS. Toxicol. Appl. Pharmacol. 181, 192-202 https://doi.org/10.1006/taap.2002.9413
  6. Hwang, S. J., J. S. Lee, B. H. Jeon, W. S. Kim, E. R. Woo and J. K. Park. 2002. Extracts of Caesalpina sappan L potentiate the apoptosis of NlH3T3 cells exposed to MMS. Kor. J. Life Sci. 12, 182-187 https://doi.org/10.5352/JLS.2002.12.2.182
  7. Iwashita, K., M. Kobori, K Yamaki and T. Tsushida. 2000. Flavonoids inhibit cell growth and induce apoptosis in B16 melanoma 4A5 cells. Biosci. Biotechnol. Biochem. 64, 1813-1820 https://doi.org/10.1271/bbb.64.1813
  8. Jung, N. C. 1998. Biological activity of urushiol and flavonoids from Lactree (Rhus verniciflua Stokes). Ph.D. thesis, Chonnam National University, Kwang-ju, South Korea
  9. Kadekaro, A. L., R. J. Kavanagh, K Wakamatsu, S. Ito, M. A. Pipitone and Z. A. Abdel-Malek. 2003. Cutaneous photobiology. The melanocyte vs. the sun: Who will win the final round? Pigment Cell Res. 16, 434-447 https://doi.org/10.1034/j.1600-0749.2003.00088.x
  10. Kawagishi, N., Y. Hashimoto, H. Takahashi, A. IshidaYamamoto and H. Iizuka. 1998. Epidermal cell kinetics of pig skin in vivo following UVB is enhanced in hyperproliferative skin condition. J. Dermatol. Sci. 18, 43-53 https://doi.org/10.1016/S0923-1811(98)00024-3
  11. Lee, J. C, H. Y. Jung and K. T. Lim. 1999. Effects of Rhus verniciflua stokes (RVS) on the plasma level of cholesterol and tumor growth in mouse. J. Taxicol. Pub. Health. 15, 169-175
  12. Lee, J. C. K. T. Lim, Y. S. Jang. 2002. Identification of Rhus verniciflua Stokes compounds that exhibit free radical scavenging and anti-apoptotic properties. Biochim. et Biophys. Acta 1570, 181-191 https://doi.org/10.1016/S0304-4165(02)00196-4
  13. Lee, J.C., J. Kim, J. K. Park, G. H. Chung, Y. S. Jang. 2003. The antioxidant, rather than prooxidant, activities of quercetin on normal cells: quercetin protects mouse thymocytes from glucose oxidase-mediated apoptosis. Exp. Cell Res. 291, 386-397 https://doi.org/10.1016/S0014-4827(03)00410-5
  14. Li. Y., M. Chopp, C. Powers and N. Jiang. 1997. Apoptosis and protein expression after focal cerebral ischemia in rat. Brain Res. 765, 301-312 https://doi.org/10.1016/S0006-8993(97)00524-6
  15. Mallikarjuna. G., S. Dhanalakshmi, R. P. Singh, C. Agarwal and R. Agarwal. 2004. Silibinin protects against photocarcinogenesis via modulation of cell cycle regulators, mitogenactivated protein kinases, and Akt signaling. Cancer Res. 64, 6349-6356 https://doi.org/10.1158/0008-5472.CAN-04-1632
  16. McGowan, C. H. and P. Russell. 2004. The DNA damage response: sensing and signaling. Curr. Opin. Cell Biol. 16, 629-633 https://doi.org/10.1016/j.ceb.2004.09.005
  17. Ichihashi, M. M. Ueda, A. Budiyanto, T. Bito, M. Oka, M. Fukunaga, K. Tsuru and T. Horikawa. 2003. UV-induced skin damage. Toxicology 189, 21-39 https://doi.org/10.1016/S0300-483X(03)00150-1
  18. Matsumura, Y. and H. N. Ananthaswamy. 2004. Toxic effects of ultraviolet radiation on the skin. Toxicol. Appl. Pharmacol. 195, 298-308 https://doi.org/10.1016/j.taap.2003.08.019
  19. Nishiwaki, Y., N. Kobayashi, K. Imoto, T. Iwamoto, A. Yamamoto, S. Katsumi, T. Shirai, S. Sugiura, Y. Nakamura, A. Sarasin, S. Miyagawa and T. Mori. 2004. Trichothiodystrophy fibroblasts are deficient in the repair of ultravioletinduced cyclobutane pyrimidine dimers and (6-4) photoproducts. J. Invest. Dermatol. 122, 526-532 https://doi.org/10.1046/j.0022-202X.2004.22226.x
  20. Otoshi, E., T. Yagi, T. Mori, T. Matsunaga, O. Nikaido, S. T. Kim, K. Hitomi, M. Ikenaga and T. Todo. 2000. Respective roles of cyclobutane pyrimidine dimers, (6-4) photoproducts, and minor photoproducts in ultraviolet mutagenesis of repair-deficient xeroderma pigmentosum A cells. Cancer Res. 60, 1729-1735
  21. Ouhtit, A., H. K. Muller, D. W. Davis, S. E. Ullrich, D. McConkey and H. N. Ananthaswamy. 2000. Temporal events in skin injury and the early adaptive responses in ultraviolet-irradiated mouse skin. Am. J. Pathol. 156, 201-207 https://doi.org/10.1016/S0002-9440(10)64720-7
  22. Read, M. A. 1995. Flavonoids: naturally occurring anti-inflammatory agent. Am. J. Pathol. 147, 235-237
  23. Renton, A., S. Llanos and X. Lu, 2003. Hypoxia induces p53 through a pathway distinct from most DNA-damaging and stress-inducing agents. Carcinogenesis 24, 1177-1182 https://doi.org/10.1093/carcin/bgg044
  24. Wu, C. H. and G. C. Yen. 2004. Antigentoxic properties of Cassia tea (Cassia tora L.): Mechanism of action and the influence of roasting process. Life Science 76, 85-101 https://doi.org/10.1016/j.lfs.2004.07.011
  25. Xu, J. and Morris, G. F. (1999). p53-mediated regulation of proliferating cell nuclear antigen expression in cells exposed to ionizing radiation. Mol. Cell. Biology. 19, 12-20
  26. Yen, G. C., D. Y. Chuang and C. H. Wu. 2002. Free radicals in foods: Chemistry, nutrition and health effects. In: Morello, M. J., F. Shahidi, and C. T. Ho (Eds), ACS Symposium Series No. 807, Washington DC, pp. 201 - 212
  27. Zhou, B. B. S. and S. J. Elledge. 2000. The DNA damage response: Putting checkpoints in perspective. Nature 408, 433-439 https://doi.org/10.1038/35044005
  28. 김태정. 1996. 한국의 자원식물(II). pp. 292-294. 서울대학교 출판부
  29. 최영전. 1992 한국민속식물. pp. 247-248. 아카데미

피인용 문헌

  1. Effect of fisetin on UVB-induced apoptosis and DNA single strand breaks in NIH3T3 cells vol.17, pp.1, 2007, https://doi.org/10.5352/JLS.2007.17.1.064
  2. Identification of catalytic acidic residues of levan fructotransferase from Microbacterium sp. AL-210 vol.17, pp.1, 2007, https://doi.org/10.5352/JLS.2007.17.1.006