Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Mitochondrial Inhibitor on Lactate Dehydrogenase of Mesocricetus auratus and Bos taurus coreanae

햄스터와 소의 젖산탈수소효소에 대한 미토콘드리아 inhibitor의 영향

  • Cho Sung Kyu (Department of Life Science, Cheongju University) ;
  • Lee Sang Hak (Department of Physical Education, Korea University) ;
  • Yum Jung Joo (Department of Life Science, Cheongju University)
  • 조성규 (청주대학교 생명유전통계학부 생명과학전공) ;
  • 이상학 (고려대학교 대학원 체육학과) ;
  • 염정주 (청주대학교 생명유전통계학부 생명과학전공)
  • Published : 2005.02.01
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Abstract

The lactate dehydrogenase (EC 1.1.1.27, LDH) inhibitors were isolated from the LDH-free crude mitochondrial fraction of skeletal muscle in Syrian hamster (Mesocricetus auratus) and Korean native cattle (Bos taurus coreanae). The LDH inhibitor in skeletal muscle of M. auratus was successfully isolated by the treatment with 175 mM NaCl and ultrasonic. The LDH inhibitor in skeletal muscle of B. taurus coreanae was highly stable to heat and LDH fu isozyme was largely inhibited by the LDH inhibitor. The molecular weight of inhibitor was 22 kDa. Inhibitor played an important role in the binding of LDH with the mitochondria in tissues of skeletal muscle, kidney and liver except heart.

젖산탈수소효소(EC 1.1.1.27, lactate dehydrogenase, LDH) inhibitor는 햄스터와 소 골격근 미토콘드리아에서 분리하였다. 햄스터 골격근조직의 LDH inhibitor는 175 mM NaCl과 초음파로 분리하였다. 소 골격근조직의 미토콘드리아에서 분리된 inhibitor는 열에 강한 특성을 보였고, $A_4$ 동위효소에 대한 저해정도가 높았으며, 분자량은 22,000 kDa으로 나타났다. Inhibitor는 심장조직을 제외한 골격근, 신장 및 간 조직의 미토콘드리아에서 LDH 결합 시 중요하게 관여하고 있었다.

Keywords

References

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