The Journal of Korean Medicine (대한한의학회지)
- Volume 26 Issue 1 Serial No. 61
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- Pages.46-58
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- 2005
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- 1010-0695(pISSN)
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- 2288-3339(eISSN)
Apoptotic Effects of Sipimikwanjung-tang of Sasang Constitutional Medicine in Human Hepatoblastoma Cells
- Song Seung-Yun (Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang university) ;
- Bae Young-Chun (Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang university) ;
- Lee Sang-Min (Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang university) ;
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Kim Kyung-Yo
(Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang university) ;
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Joo Jong-Cheon
(Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang university) ;
- Ko Ki-Duk (Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang university) ;
- Park Soo-Jeong (Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang university) ;
- Lee Kyung-Sung (Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang university) ;
- Choi Yong-Seok (Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang university) ;
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Kim Jong-Yeol
(Korea Institute of Oriental Medicine)
- Published : 2005.03.01
Abstract
Objective : This study on Sipimikwanjung-tang was undertaken to evaluate its antioxidant capacities and antiperoxidation activities in rat liver tissues. Sipimikwanjung-tang which has been one of the prescriptions in sasang constitutional medicine is usually applied for the therapy of various liver diseases. It is elucidated that Sipimikwanjung-tang has antioxidants on liver tissue of rat and the cytotoxic effects on human hepatoblastoma Hep G2 cells. Methods: Sipimikwanjung-tang extract in antioxidant effects of Hep G2 cells is evaluated by MTT assay, DAPI staining, DNA fragmentation assays and FACS can analysis. Results: Sipimikwanjung-tang induced apoptosis in Hep G2 cells, and induced G1 and G2M arrest of the cell cycle as well as a significant increase in PARP and caspase-3 activity. It induced an increase in
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