Biomedical Science Letters (대한의생명과학회지)
- Volume 11 Issue 1
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- Pages.51-56
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- 2005
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- 1738-3226(pISSN)
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- 2288-7415(eISSN)
Human Papillomavirus Testing with Hybrid Capture II and DNA Chip
- Im Jee-Aee (Department of Laboratory Medicine, MizMedi Hospital) ;
- Shim Moon-Jung (Department of Clinical Pathology, Ansan College) ;
- Ryang Yong-Suk (Department of Biomedical Laboratory Science and Institute of Health Science, College of Health Science, Yonsei University) ;
- Lee Duk-Chul (Department of Family Medicine, Young-dong Severance Hospital)
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- 이덕철 (영동 세브란스 병원 가정의학과)
- Published : 2005.03.01
Abstract
The detection of high-risk human papilloma virus (HPV) allows us to predict the presence and future development of cervical intraepitheliallesion. In this study, we compared Hybrid Capture II and DNA chip methods for detection of HPV in cervical swab samples. And we evaluated the clinical efficacy and diagnostic performance of HPV DNA chip and Hybrid Capture II for detecting HPV in cervical neoplastic lesions. Seventy four patients were classified into three groups according to their histologic diagnosis: Group I (nonspecific chronic cervicitis), Group II (low-grade squamous intraepithelial lesion (SIL); koilocytosis, and mild dysplasia), and Group III (high-grade SIL;, moderate, severe dysplasia and in situ carcinoma). Cytologic diagnosis were based on the Bethesda System. Hybrid Capture II and DNA chip methods were performed to detect HPV. In 41 of the 74 cervical samples