Biomedical Science Letters (대한의생명과학회지)
- Volume 11 Issue 1
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- Pages.9-13
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- 2005
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- 1738-3226(pISSN)
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- 2288-7415(eISSN)
Genotypic Characterization of Salmonella enterica Serotype Enteritidis Isolated from Food-Poisoning Cases and Chickens by Pulsed Field Gel Electrophoresis
- Suh Dong Kyun (Research Institute of Health and Environment) ;
- Song Jae Chan (College of Veterinary Medicine, Kyungpook National University)
- Published : 2005.03.01
Abstract
A total of 22 Salmonella enterica serotype Enteritidis (S. Enteritidis) isolates from human and chicken sources were analyzed by pulsed field gel electrophoresis (PFGE) using XbaI restriction enzyme to assess the genetic relationships between strains from different sources. PFGE permitted the resolution of XbaI restriction fragments of the 22 S. Enteritidis into 6 distinct PFGE types (PFT), designated PFT1 to PFT6, and 2 subtypes within PFT2, and allowed to detect between 9 and 10 bands with fragments sizes in the range of